PURPOSE: To obtain the title medium of culturing yeast belonging to the genus Lipomyces while maintaining spore and sporsac forming ability, containing a specific compound such as aspartic acid as a nitrogen source.

CONSTITUTION: The objective medium containing at least one of a compound selected from aspartic acid, glutamic acid, urea, guanine, valine, tyrosine, lysine, ammonium carbonate, glycine, threonine, glutamine, histidine, ammonium chloride, alanine, tryptophan, asparagine, arginine, proline and xanthine. A hexose and an oligosaccharide and a polysaccharide thereof and glycerol are preferable as a carbon source.