Title:
重合反応のための合成核酸
Document Type and Number:
Japanese Patent JP6572268
Kind Code:
B2
Abstract:
Compositions and methods are provided for inhibiting a polymerase from replicating non target DNA at a temperature below the amplification reaction temperature. The inhibitor is a synthetic nucleic acid which is single stranded but folds to form at least one double stranded region designed to melt at a temperature which is lower than the amplification reaction temperature, and at least one single stranded region where the single stranded region at the 5' end contains at least one uracil or inosine and optionally a sequence at the 3' end contains one or more derivative nucleotide or linkages.
Inventors:
Jennifer on
Donald Johnson
Thomas Sea Evans
Lucia Greenough
Donald Johnson
Thomas Sea Evans
Lucia Greenough
Application Number:
JP2017155557A
Publication Date:
September 04, 2019
Filing Date:
August 10, 2017
Export Citation:
Assignee:
New England Bio-Rave's Incorporated
International Classes:
C12N15/09; C12Q1/6848; C12Q1/686
Domestic Patent References:
| JP2003504018A | ||||
| JP2009509565A | ||||
| JP2008516613A | ||||
| JP2000508538A | ||||
| JP2005523016A | ||||
| JP9220087A | ||||
| JP2015512651A |
Other References:
BMC Molecular Biology,2011年,Vol.12,p.1-12(Article No.6)
Journal of Inorganic Biochemistry,2008年,Vol.102,p.1104-1111
Journal of Molecular Biology,2006年,Vol.363,p.244-261
Journal of Inorganic Biochemistry,2008年,Vol.102,p.1104-1111
Journal of Molecular Biology,2006年,Vol.363,p.244-261
Attorney, Agent or Firm:
Kawaguchi International Patent Office