Title:
SYSTEMS AND METHODS FOR IN VIVO DUAL RECOMBINASE-MEDIATED CASSETTE EXCHANGE (dRMCE) AND DISEASE MODELS THEREOF
Document Type and Number:
Japanese Patent JP2022091801
Kind Code:
A
Abstract:
To provide donor vectors and systems for producing consistent and correct animal models of transgene expression.SOLUTION: Provided is a system comprising: a promoter-less donor vector comprising a polyadenylation signal or transcription stop element upstream of a transgene or RNA, the transgene or RNA, and paired recombinase recognition sites; and one expression vector comprising two genes encoding recombinases specific to the paired recombinase recognition sites, or two expression vectors, the first expression vector comprising one gene encoding a first recombinase that is specific to one of the paired recombinase recognition sites, and the second expression vector comprising one gene encoding a second recombinase that is specific to the other of the paired recombinase recognition sites.SELECTED DRAWING: Figure 12
Inventors:
JOSHUA BREUNIG
MOISE DANIELPOUR
KIM GI BUM
MOISE DANIELPOUR
KIM GI BUM
Application Number:
JP2022036725A
Publication Date:
June 21, 2022
Filing Date:
March 10, 2022
Export Citation:
Assignee:
CEDARS SINAI MEDICAL CENTER
International Classes:
C12N15/85; A01K67/027; A61K35/12; A61K35/545; A61K48/00; A61P43/00; C12N5/074; C12N5/10; C12N15/113
Foreign References:
| WO2011001247A2 | 2011-01-06 | |||
| WO2014204724A1 | 2014-12-24 |
Other References:
BIOTECHNIQUES (2013) VOL.55, NO.5, PP.233-242, JPN6020046795, ISSN: 0005018638
PATHOGENETICS (2008) VOL.1, NO.3, PP.1-10, JPN6020046793, ISSN: 0005018639
NATURE METHODS (2010) VOL.7, NO.11, PP.893-895, ONLINE METHODS, JPN6020046794, ISSN: 0005018640
PATHOGENETICS (2008) VOL.1, NO.3, PP.1-10, JPN6020046793, ISSN: 0005018639
NATURE METHODS (2010) VOL.7, NO.11, PP.893-895, ONLINE METHODS, JPN6020046794, ISSN: 0005018640
Attorney, Agent or Firm:
Shimizu Hatsushi
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto