Title:
液体サンプル内の磁気応答性粒子を操作し、サンプルからDNA又はRNAを収集するシステム及び方法
Document Type and Number:
Japanese Patent JP4792192
Kind Code:
B2
Abstract:
A system and method for manipulating magnetic particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and paramagnetic particles. The device includes heating and cooling devices to facilitate a lysing step to release the nucleic acid molecules from the cells in the cell solution. The device further includes moveable magnets which can be moved proximate to and away from the tube to hold the paramagnetic particles to which the nucleic acid molecules become bound, so that the molecule-bound particles can be separated from the remainder of the solution, and washed as appropriate. The system also employs an electromagnet which is capable of demagnetizing the particles to allow the particles to freely mix with solution, such as elution solutions which are used to unbind the molecules from the particles.
Inventors:
Timothy Hansen
Bradley Thomas
John Bianco
Matthew Corris
Bradley Thomas
John Bianco
Matthew Corris
Application Number:
JP2001585934A
Publication Date:
October 12, 2011
Filing Date:
May 17, 2001
Export Citation:
Assignee:
BECTON, DICKINSON AND COMPANY
International Classes:
B03C1/00; C12N15/09; B01D43/00; B01L7/00; B03C1/28; C12M1/00; C12M1/38; C12N15/10; G01N35/00; G01N35/02; G01N35/10
Domestic Patent References:
| JP2000070755A | ||||
| JP53136764A | ||||
| JP11156231A | ||||
| JP11326338A |
Attorney, Agent or Firm:
Yoshikazu Tani
Kazuo Abe
Kazuo Abe