Title:
TARGETED REPLACEMENT OF ENDOGENOUS T CELL RECEPTORS
Document Type and Number:
Japanese Patent JP2022137112
Kind Code:
A
Abstract:
To provide methods of editing the genome of a human T cell.SOLUTION: The present disclosure provides a method of editing the genome of a human T cell, the method comprising inserting a nucleic acid sequence into a target region in exon 1 of a T cell receptor (TCR)-subunit constant gene in the human T cell, a nucleic acid sequence encoding from a N-terminus to a C-terminus, (i) a first self-cleaving peptide sequence; (ii) a first heterologous TCR subunit chain comprising the variable region and the constant region of the TCR subunit; (iii) a second self-cleaving peptide sequence; (iv) a variable region of a second heterologous TCR subunit chain; and (v) a portion of the N-terminus of the endogenous TCR subunit, wherein, if the endogenous TCR subunit is TCR-α, the first subunit is TCR-β, and the second subunit is TCR-α, and wherein if the endogenous TCR subunit is TCR-β, the first is TCR-α, and the second is TCR-β.SELECTED DRAWING: Figure 1a
Inventors:
Los Theodore Lee
Sifult Eric
Marson Alexander
Puig South Christina
Rivas Antoni
Sifult Eric
Marson Alexander
Puig South Christina
Rivas Antoni
Application Number:
JP2022103225A
Publication Date:
September 21, 2022
Filing Date:
June 28, 2022
Export Citation:
Assignee:
The Regents of the University of California
International Classes:
C12N15/09; A61K35/17; A61K48/00; A61P35/00; C12N5/10
Foreign References:
WO2017106528A2 | 2017-06-22 | |||
WO2014153470A2 | 2014-09-25 |
Other References:
PNAS, vol. 112, JPN6021048863, 2015, pages 10437 - 10442, ISSN: 0005100589
NATURE, vol. 543, JPN6021048865, 2 March 2021 (2021-03-02), pages 113 - 117, ISSN: 0005100590
NATURE, vol. 543, JPN6021048865, 2 March 2021 (2021-03-02), pages 113 - 117, ISSN: 0005100590
Attorney, Agent or Firm:
Shimizu Hatsushi
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto