Title:
【発明の名称】細胞分離材及び分離器
Document Type and Number:
Japanese Patent JP2817934
Kind Code:
B2
Abstract:
PURPOSE:To enable simplified and rapid operation of cell separation at a low cost by filling the filter with a separation sheet which is made of a polyvinyl acetal resin containing open cells of 10 to 1,000mum average pore size. CONSTITUTION:A polyvinyl acetal resin including open cells of 10 to 1,000mum average pore size and having 50 to 100% acetalization such as polyvinyl formal or polyvinyl butylal resin is processed into a sheet of a sponge structure as a cell body separator. For example, this sheet is punched into a disk of an appropriate diameter corresponding to the inner diameter of the column to prepare the separator which is set in the column. The cell suspension is poured from the inlet 3 into the separator 1 so that the separator 2 is sufficiently soaked. Then, the rinsing solution is poured to rinse off the unadsorbed cells from outlet 4 and they are recovered. Thus, the separator of very high stability as well as high separative power is obtained and the cells can be separated without change in their population.
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Inventors:
Hiraide Tsuneo
Akihiko Yokoo
Kenji Ichizuka
Akihiko Yokoo
Kenji Ichizuka
Application Number:
JP4800089A
Publication Date:
October 30, 1998
Filing Date:
February 28, 1989
Export Citation:
Assignee:
Asahi Optical Co., Ltd.
International Classes:
C12N5/00; A61K35/14; A61K35/17; C12N5/04; C12N5/07; C12N5/071; (IPC1-7): C12N5/06
Domestic Patent References:
| JP62201939A | ||||
| JP6475014A |
Attorney, Agent or Firm:
Kunio Miura