PURPOSE: To clone the heterologous gene factor to various other microbial strains at the same time, using a vector comprising the plasmid of a novel Bacillus stearothermophilus having a plasmid containing a neomycin- and kanamycin-resistance gene, having a molecular weight of about 3 M-Dalton and represented by a specific restriction enzyme map.
CONSTITUTION: Soil is cultured in a TYS medium under shaking, and subjected to the plate culture on a TYS agar medium containing neomycin and kanamycin to obtain the colopy of a novel Bacillus stearothermophilus N1 strain (FERM-P No.6660). The strain contains a plasmid having the neomycin- and kanamycin- resistance gene, and a molecular weight of about 3 M-Dalton, and represented by the restriction enzyme map of the figure. A heterologous gene can be cloned to Bacillus subtilis and a thermophilic strain at the same time by using the plasmid as a vector.
TOMIZUKA NOBORU
IKEDA TAKAYUKI
NARISHIMA HIROYUKI
