Title:
【発明の名称】脱水リポソ-ム
Document Type and Number:
Japanese Patent JPH0818973
Kind Code:
B2
Abstract:
Dehydrated liposomes are prepared by drying liposome preparations under reduced pressure in the presence of one or more protective sugars, e.g., the disaccharides trehalose and sucrose. Preferably, the protective sugars are present at both the inside and outside surfaces of the liposome membranes. Freezing of the liposome preparation prior to dehydration is optional. Alternatively, the protective sugar can be omitted if : (1) the liposomes are of the type which have multiple lipid layers ; (2) the dehydration is done without prior freezing ; (3) the dehydration is performed to an end point which results in sufficient water being left in the preparation (e.g., at least 12 moles water/mole liped) so that the integrity of a substantial portion of the multiple lipid layers is retained upon rehydration. Concentration gradients capable of generating transmembrane potentials can be created across the liposome membranes either before or after dehydration, and the transmembrane potentials resulting from these gradients can be used to load charged materials, e.g., drugs, into the liposomes.
Inventors:
Yanotsuf, Andrew, S
Cucumber, Peter, Earl
Barry, marcel, bee
Fountain, michael, dubril
Ginsberg, Lichyard, Es
Hope, Michael, Jei
Matsuden, Thomas, Day
Shiren, Hiyu, Pee
Yabronski, Regina, El
Cucumber, Peter, Earl
Barry, marcel, bee
Fountain, michael, dubril
Ginsberg, Lichyard, Es
Hope, Michael, Jei
Matsuden, Thomas, Day
Shiren, Hiyu, Pee
Yabronski, Regina, El
Application Number:
JP50363085A
Publication Date:
February 28, 1996
Filing Date:
August 07, 1985
Export Citation:
Assignee:
The Liposome, Kampani, Inco-Polle Ted
International Classes:
A61K9/00; A61K9/10; A61K9/127; A61K31/435; C07D475/08; A61K31/505; A61K31/519; A61K31/70; A61K31/7028; A61K31/7034; A61K31/704; A61K31/7042; A61K31/7052; A61K31/7064; A61K31/7068; A61K31/715; A61K45/00; A61K45/08; A61K47/26; A61K47/36; A61K47/48; A61P35/00; B01J13/02; B01J13/04; C07D519/00; C07H15/252; C07H19/09; (IPC1-7): A61K9/127; A61K47/26; A61K47/36; B01J13/02
Domestic Patent References:
| JPS5449317A | 1979-04-18 | |||
| JPS567714A | 1981-01-27 | |||
| JPS574913A | 1982-01-11 | |||
| JPS5976021A | 1984-04-28 | |||
| JPS6143110A | 1986-03-01 |
Attorney, Agent or Firm:
Akitoshi Otawa (1 outside)
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