Title:
【発明の名称】ウイルス仲介DNA導入の増強
Document Type and Number:
Japanese Patent JP3411039
Kind Code:
B2
Abstract:
A method to increase the efficiency of transduction of hematopoietic and other cells by retroviruses includes infecting the cells in the presence of fibronectin or fibronectin fragments. As can be seen in the figure the fibronectin and fibronectin fragments significantly enhance retroviral-mediated gene transfer into the cells, particularly hematopoietic cells including committed progenitors and primitive hematopoietic stem cells. The invention also provides improved methods for somatic gene therapy capitalizing on enhanced gene transfer, hematopoietic cellular populations, and novel constructs for enhancing retroviral-mediated DNA transfer into cells and their use.
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Inventors:
William A, David A
Patel, Vikram Pee
Patel, Vikram Pee
Application Number:
JP52525795A
Publication Date:
May 26, 2003
Filing Date:
March 27, 1995
Export Citation:
Assignee:
Advanced Research and Technology Institute
Northwestern University
Northwestern University
International Classes:
C12N15/09; A01K67/027; A61K35/28; A61K35/76; A61K38/00; A61K38/04; A61K38/16; A61K38/22; A61K38/27; A61K38/46; A61K39/395; A61K47/48; A61K48/00; A61P7/00; A61P35/00; A61P43/00; C07H21/04; C07J1/00; C07K14/435; C07K14/47; C07K14/78; C07K17/14; C07K19/00; C12M3/00; C12N5/10; C12N9/78; C12N15/48; C12N15/63; C12N15/867; (IPC1-7): C12N15/09; A61K35/76; A61K47/48; A61K48/00; C07K14/78
Domestic Patent References:
| JP2311498A | ||||
| JP297397A |
Other References:
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Attorney, Agent or Firm:
Kazuo Shamoto (5 outside)