Title:
【発明の名称】平衡型構成誘導性転写系
Document Type and Number:
Japanese Patent JP2769541
Kind Code:
B2
Abstract:
A balanced constitutive inducible transcriptional control system is provided to facilitate the secretion of polypeptides incompatible with recombinant host cells. Use of this system has, for the first time, made it possible to obtain mature, unglycosylated human tissue plasminogen activator from prokaryotes which is water soluble and fully enzymatically active. In accordance with this invention, t-PA is produced by recombinant bacterial host cell culture in commercially significant amounts without in vitro renaturation and processing. This system is optimally used with a microbial-mammalian cell secretion shuttle vector in a novel process for screening preprotein gene variants. Also provided is a novel t-PA variant DNA having a silent mutation in the 5' end of the coding sequence which results in elevated levels of microbial secretion.
Inventors:
Mirror, harvey eye
Application Number:
JP50640688A
Publication Date:
June 25, 1998
Filing Date:
June 23, 1988
Export Citation:
Assignee:
Genentech, Inc.
International Classes:
C07K14/61; C12N1/21; C12N5/10; C12N9/72; C12N15/09; C12N15/11; C12N15/67; C12N15/70; C12N15/85; C12N9/64; C12P21/00; C12R1/19; C12R1/91; (IPC1-7): C12P21/00; C12N1/21; C12N5/10; C12N9/64; C12N15/09
Domestic Patent References:
| JPS61100197A | 1986-05-19 | |||
| JPS5942321A | 1984-03-08 |
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)