Title:
【発明の名称】真菌のβ-チューブリン遺伝子
Document Type and Number:
Japanese Patent JP2002541782
Kind Code:
A
Abstract:
The anticancer drug taxol binds to beta-tubulin in assembled microtubules (MT) and causes cell cycle arrest in animal cells; in contrast, the effect of taxol varies in fungi. For instance, the taxol-producer Pestalotiopsis microspora Ne32, an ascomycete, is resistant to taxol (1C50>11.7 M), whereas Pythium ultimum and Phytophthora cinnamomi, two oomycetes, are sensitive to taxol (IC50 0.1 mu M). cDNAs encoding beta-tubulin from P.microspora, P. ultimum, and P. cinnamomi were isolated. The deduced amino acid sequences of beta-tubulin from P. microspora, P.ultimum, and P. cinnamomi can be used in (1) binding assays for the detection of taxol and taxol-like substances; (2) diagnostic assays for the pharmacologic efficacy of taxol against a tumor sample; (3) designing drugs with taxol-like activity via application of information regarding the effect of specific residues on taxol binding; and (4) detection of taxol and taxol-like activity via use of taxol-sensitive and taxol-resistant isogenic strains of P.ultimum constructed by substitution of residues necessary for taxol binding.
Inventors:
Sidu, La Jinda, Es
Baron, Eartha, Pee
Mu, Jing Hong
Baron, Eartha, Pee
Mu, Jing Hong
Application Number:
JP2000606753A
Publication Date:
December 10, 2002
Filing Date:
March 23, 2000
Export Citation:
Assignee:
Exagenics, ink
International Classes:
G01N33/50; A01N43/20; A01N63/00; A01N63/04; A61K45/00; A61P31/10; A61P35/00; C07K14/37; C12N1/13; C12N15/09; C12N15/31; C12Q1/02; G01N33/15; G01N33/53; G01N33/566; G01N33/569; (IPC1-7): C12N15/09; A01N43/20; A01N63/00; A01N63/04; A61K45/00; A61P31/10; A61P35/00; C07K14/37; C12N1/13; C12Q1/02; G01N33/15; G01N33/50; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Sanada Yuzo (2 outside)