Title:
【発明の名称】化学発光性アクリジニウム化合物を使用するヒドリドの測定
Document Type and Number:
Japanese Patent JP2002530678
Kind Code:
A
Abstract:
The present invention discloses a method for the measurement of hydride using a chemiluminescent compound, such as an acridinium compound. The source of hydride for the reduction of acridinium compound may be of chemical or biochemical origin, or the result of enzymatic catalysis. The chemical source of hydride, might be NaBH4. A biochemical source of hydride might be that derived from NADH, or NADPH, while an enzymatic source would be the class of oxidoreductases termed dehydrogenases which convert NADH or NADPH from NAD or NADP. Among applications for acridinium compounds as chemiluminescent indicators of hydride are diagnostic assays using dehydrogenases as reagents, indicators, diagnostic markers or as labels. Ethanol, for example, might be detected with acridinium ester chemiluminescence through the reaction of alcohol dehydrogenase on ethanol, said reaction producing NADH. As a label, dehydrogenase might be used in an ELISA for the detection of a specific analyte with acridinium ester providing the signaling response.
Inventors:
Sharp, david
Natrajan, Anand
Gian, Quinnpin
Parsons, George
Law, Say John
Natrajan, Anand
Gian, Quinnpin
Parsons, George
Law, Say John
Application Number:
JP2000584306A
Publication Date:
September 17, 2002
Filing Date:
November 24, 1999
Export Citation:
Assignee:
Bayer Corporation
International Classes:
G01N31/00; C07D219/04; C12Q1/00; G01N21/78; G01N33/58; (IPC1-7): G01N31/00; C07D219/04; G01N21/78
Domestic Patent References:
| JPH0296567A | 1990-04-09 | |||
| JPS63112564A | 1988-05-17 |
Attorney, Agent or Firm:
Shusaku Yamamoto