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Title:
【発明の名称】線虫類の培養方法
Document Type and Number:
Japanese Patent JP2842580
Kind Code:
B2
Abstract:
PCT No. PCT/JP94/01204 Sec. 371 Date Jan. 29, 1996 Sec. 102(e) Date Jan. 29, 1996 PCT Filed Jul. 21, 1994 PCT Pub. No. WO95/02958 PCT Pub. Date Feb. 2, 1995The present invention relates to a method of cultivating, in medium, Steinernema kushidai (hereinafter simply called kushidai) which is one type of Nematoda. Kushidai has a strong insecticidal ability to kill larvae of coliopterous insects, and cultivation of large quantities of kushidai having the strong insecticidal ability requires conditions different from the techniques of cultivating other nematodes parasitic on insects. Kushidai is cultivated in a medium including asteroid. This increases the insecticidal activity with respect to insects harmful to plants such as crops. The medium including asteroid may be adsorbed to a plurality of supports, and then the supports may be stacked with gaps thereamong to form a layer. This enables kushidai to be cultivated efficiently. Further, a liquid medium including asteroid may be prepared, with cultivation carried out while agitating the medium and supplying oxygen thereto. This obtains kushidai having a high multiplication characteristic and high insecticidal activity. The kushidai is used as a biological agrochemical to reduce damage done to crops such as sweet potato by harmful insects.

Inventors:
TACHIBANA MINEO
UECHI TOSHIHITO
SUZUKI NOBUKAZU
KAWASUGI TADAAKI
Application Number:
JP18230793A
Publication Date:
January 06, 1999
Filing Date:
July 23, 1993
Export Citation:
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Assignee:
KUBOTA KK
International Classes:
A01K67/033; (IPC1-7): A01K67/033; A23K1/165
Domestic Patent References:
JP6463326A
JP61503001A
Other References:
Nematologica,「39」 2,(1993),p.266−273
Attorney, Agent or Firm:
Shuichiro Kitamura