Title:
【発明の名称】組換えDNA誘導型梅毒トレポネ-マ抗原
Document Type and Number:
Japanese Patent JPS59502131
Kind Code:
A
Abstract:
DNA fragments from Treponema pallidum are packaged into suitable bacteriophage vectors for propagation within E. Coli. The resulting plaques are screened for antigens reactive with antibodies to Treponema pallidum. Clones from positive plaques are further propagated and purified to provide antigens useful in the serodiagnosis and study of syphilis and as vaccines against syphilis infection. Plasmid subclones of treponemal antigen producing phage clones are propagated in E. coli to provide an alternate source of treponemal antigens. In addition to bacteriophage clones which encode for polypeptide treponemal antigens, a clone is also disclosed which encodes for a protease resistent heat labile treponemal antigen.
Inventors:
Robert Michael A.
Application Number:
JP50017484A
Publication Date:
December 27, 1984
Filing Date:
November 04, 1983
Export Citation:
Assignee:
The Re-The Enz of University of California
International Classes:
C12N15/00; A61K39/02; C07K14/00; C07K14/195; C07K14/20; C07K14/41; C12N1/20; C12N7/00; C12N15/09; C12P21/00; C12Q1/00; G01N33/571; A61K39/00; (IPC1-7): C12P21/00; A61K39/02; C07G7/00; C12N1/20; C12N7/00; C12N15/00; G01N33/54; @@@@@@@/@@@
Attorney, Agent or Firm:
Soichi Takezawa