Title:
【発明の名称】組換えセコイソラリシレシノールデヒドロゲナーゼ、および使用方法
Document Type and Number:
Japanese Patent JP2002512790
Kind Code:
A
Abstract:
A secoisolanciresinol dehydrogenase protein has been isolated from Forsythia intermedia, together with cDNAs encoding secoisolariciresinol dehydrogenase from this species. Accordingly, isolated DNA sequences are provided which code for the expression of secoisolariciresinol dehydrogenase. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a secoisolariciresinol dehydrogenase protein, or to a base sequence sufficiently complementary to at least a portion of a secoisolariciresinol dehydrogenase DNA or RNA to enable hybridization therewith. Thus, systems and methods are provided for the recombinant expression of secoisolariciresinol dehydrogenases that may be used to facilitate the production, isolation and purification of significant quantities of recombinant secoisolariciresinol dehydrogenase for subsequent use, to obtain expression or enhanced expression of secoisolariciresinol dehydrogenase in plants in order to enhance, or otherwise alter, lignan biosynthesis, or may be otherwise employed for the regulation or expression of secoisolariciresinol dehydrogenase.
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Inventors:
Zaire, Zuhi-Kian
Costa, Michael A.
Dubin, Lawrence Bee.
Lewis, Norman Gee.
Costa, Michael A.
Dubin, Lawrence Bee.
Lewis, Norman Gee.
Application Number:
JP2000545990A
Publication Date:
May 08, 2002
Filing Date:
April 23, 1999
Export Citation:
Assignee:
Washington State University Research Foundation
International Classes:
C12N15/09; C07H21/04; C12N1/15; C12N1/19; C12N1/20; C12N1/21; C12N5/00; C12N5/10; C12N9/04; C12N15/00; C12P21/02; C12Q1/68; (IPC1-7): C12N9/04; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N5/00; C12N15/09; C12P21/02
Attorney, Agent or Firm:
Shusaku Yamamoto