Title:
【発明の名称】酵母による外因性ポリペプチドの分泌
Document Type and Number:
Japanese Patent JPS60501140
Kind Code:
A
Abstract:
Disclosed are recombinant methods and materials for use in securing production of exogenous (e.g., mammalian) polypeptides in yeast cells wherein hybrid precursor peptides susceptible to intracellular processing are formed and such processing results in secretion of desired polypeptides. In a presently preferred form, the invention provides transformation vectors with DNA sequences coding for yeast synthesis of hybrid precursor polypeptides comprising both an endogenous yeast polypeptide sequence (e.g., that of a precursor polypeptide associated with yeast-secreted mating factor alpha and an exogenous polypeptide sequence (e.g., human beta -endorphin). Transformation of yeast cells with such DNA vectors results in secretion of desired exogenous polypeptide (e.g., substances displaying one or more of the biological properties of beta -endorphin).
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Inventors:
Bitter, Grant A.
Application Number:
JP50181784A
Publication Date:
July 25, 1985
Filing Date:
April 18, 1984
Export Citation:
Assignee:
Am Jien
International Classes:
C07K14/675; C12N15/09; C12N15/62; C12N15/00; C12N15/81; C12P21/00; C12P21/02; C12R1/865; (IPC1-7): C12N15/00; C12P21/02; C12R1/865
Domestic Patent References:
| JPS5519092A | 1980-02-09 | |||
| JPS56164200A | 1981-12-17 | |||
| JPS59132892A | 1984-07-31 |
Attorney, Agent or Firm:
Yoshihiro Tsunoda