PURPOSE: To reduce a level of noise due to pulsatory flow and to permit detection of adrenalin of small amount by a construction wherein fluorescence sensitive reaction solutions are supplied into a flow tube for the column effluent of the chromatograph using a reciprocative plural-pump so that those solutions are applied to the flow tube at plural positions.
CONSTITUTION: An effluent from a separation column 2 of the liquid chromatograph for measuring catecholamine is sent to a flow tube in a fluorescence sensitive portion 3. Potassium ferricyanide aqueous solutionI, ascorbic acid aqueous solution II and NaOH aqueous solution III are respectively supplied through line tubes 14, 15 and 16 to plural positions of the flow tube 4 using a reciprocative plural-pump 5, thus avoiding occurrnce of pulsatory flow. In this way, a level of noise produced in the fluorescence detecting portion due to the pulsatory flow is reduced so much to permit detection of catecholamine, particularly adrenalin of very small amount (4 pico gram).
YAMAZAKI KAZUTOSHI
KAMINOYAMA TOMOHITO
| JPS524894A | 1977-01-14 | |||
| JPS55122149A | 1980-09-19 |