Title:
【発明の名称】チモーゲン活性化系
Document Type and Number:
Japanese Patent JP2003508045
Kind Code:
A
Abstract:
We describe the DNA sequences encoding an expression vector system that will permit, through limited proteolysis, the activation of expressed zymogen precursor of (S1) serine proteases in a highly controlled and reproducible fashion. The processed expressed protein, once activated, is rendered in a form amenable to measuring the catalytic activity. This catalytic activity of the activated form, is often a more accurate representation of the mature S1 protease gene product relative to the unprocessed zymogen precursor. Thus, this series of zymogen activation constructs represents a significant system for the analysis and characterization of serine protease gene products.
Inventors:
Darrow, Andrew
Chi, chien sen
Andrei-Gordon, Patricia
Chi, chien sen
Andrei-Gordon, Patricia
Application Number:
JP2001520837A
Publication Date:
March 04, 2003
Filing Date:
August 14, 2000
Export Citation:
Assignee:
Ortho-McNeill Pharmacy Incorporated
International Classes:
A61K8/00; A61K8/66; A61K38/48; A61K47/48; A61P15/00; A61P17/00; A61P25/00; A61P29/00; A61P43/00; A61Q5/02; C11D3/386; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/50; C12N9/64; C12N15/57; C12N15/09; C12N15/62; C12P21/06; C12Q1/37; G01N33/15; G01N33/50; G01N33/58; A61K38/00; (IPC1-7): C12N15/09; A61K7/075; A61K38/48; A61P15/00; A61P17/00; A61P25/00; A61P29/00; A61P43/00; C11D3/386; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/50; C12Q1/37; G01N33/15; G01N33/50; G01N33/58
Attorney, Agent or Firm:
Yoshio Kawaguchi (4 outside)