Title:
【発明の名称】微生物の抗菌感受性試験のための装置および方法
Document Type and Number:
Japanese Patent JPH04502707
Kind Code:
A
Abstract:
Method and apparatus for testing the qualitative susceptibility of a microorganism to growth inhibition by an antimicrobial product utilizing a test panel (100-1). Test modules (107, 108, 109) are carried in a negative growth control well (101), a positive growth control well (102), and a test well (103). The test modules comprise a dry solid volume of a subset of the constituents of a set of test chemicals comprising resazurin and growth medium. The test module (109) in the test well further comprises a volume of antimicrobial product. The test module (107) in the negative growth control well is rehydrated by a volume of sterile liquid (110) containing all of the other test chemicals not present in the test module. The test modules (108, 109) in the positive growth control well and in the test well are rehydrated by a volume of inoculum liquid (112, 113) containing all those other constituents together with a concentration of microorganism. The test panel (100-1) is incubated and then read using a visible light reading protocol or a fluorescence excitation reading protocol to determine growth or no growth in the wells. The visible light reading protocol determines organism growth by presence of a color shift from blue toward red. Test modules are preferably paper disks with test constituents absorbed therein and the concentration of antimicrobial product printed thereon.
Inventors:
Lancaster, Michael Buoy
Fields, Rebecca Day
Fields, Rebecca Day
Application Number:
JP50307690A
Publication Date:
May 21, 1992
Filing Date:
January 16, 1990
Export Citation:
Assignee:
Accumed International, Inc.
International Classes:
B01L3/00; C12M1/34; G01N33/15; C12Q1/18; (IPC1-7): C12M1/34; C12Q1/18
Attorney, Agent or Firm:
Yoshiyuki Inaba (2 outside)