Title:
【発明の名称】ジオキセタン標識プローブ及び同プローブを使用する検出検定
Document Type and Number:
Japanese Patent JP2002508654
Kind Code:
A
Abstract:
Probes labeled with 1,2-dioxetane precursors can be employed in a variety of assays. The probes may be nucleic acid, peptide nucleic acid, proteins including enzyme, antibody or antigen, steroid, carbohydrate, drug or non-drug hapten. The probe is provided with a 1,2-dioxetane precursor bound thereto, generally either covalently, or a strong ligand bond. The dioxetane precursor moiety is converted to a bound 1,2-dioxetane by exposure to singlet oxygen. These dioxetane (labels) either spontaneously decompose, or are induced to decompose by an appropriate trigger to release light. The trigger may be a change in pH temperature, or an agent which removes a protective group. Assay formats in which these 1,2-dioxetane labeled probes and referents may be used to include hybridization assays, immuno assays, gel-based assays and Capillary Zone Electrophoresis.
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Inventors:
Bronstein, Irena
Edwards, brooks
Martin, christopher
John Voita
Edwards, brooks
Martin, christopher
John Voita
Application Number:
JP52775698A
Publication Date:
March 19, 2002
Filing Date:
December 11, 1997
Export Citation:
Assignee:
TROPIX,INC.
International Classes:
G01N33/532; C07D321/00; C07H21/00; C07H21/02; C07H21/04; C12N15/09; C12Q1/68; (IPC1-7): C12Q1/68; C07D321/00; C07H21/02; C07H21/04; C12N15/09; G01N33/532
Attorney, Agent or Firm:
Hajime Tsukuni (1 person outside)