【発明の名称】遺伝子操作によるイソアミルアルコールの多生成法並びに遺伝子操作による清酒の製造法及び清酒中のイソアミルアルコールの含有量調整法

Title:

Document Type and Number:

Japanese Patent JP2926190

Kind Code:

B2

Abstract:

PURPOSE:To form a large amount of isoamyl alcohol by transforming Saccharomyces cerevisiae with an architectural plasmid having an inserted gene encoding an amino acid sequence of enzyme alpha-isopropyl malate synthase. CONSTITUTION:A gene (LEU4) encoding an amino acid sequence of enzyme alpha-isopropyl malate synthase is separated from Saccharomyces cerevisiae with an architectural plasmid having an inserted gene encoding AH22 strain by using multi-copy type plasmid pY11, the gene is inserted into a multi-copy type or single copy type plasmid, Saccharomyces cerevisiae is transformed with an architectural plasmid thereof and a large amount of isoamyl alcohol is produced by using the transformed Saccharomyces cerevisiae or the amount of isoamyl alcohol produced is controlled.

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Inventors:

HIRATA MASARU
HIROI TADAO
SUZUKI TSUNEO
TSUKIOKA MOTO

Application Number:

JP12567290A

Publication Date:

July 28, 1999

Filing Date:

May 16, 1990

Export Citation:

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Assignee:

NIIGATAKEN

International Classes:

C12P7/04; C12G3/02; C12N1/19; C12N15/09; C12R1/865; (IPC1-7): C12N15/09; C12G3/02; C12P7/04

Other References:

日本農芸化学会誌、第６４巻、第３号、第４１６頁「３Ｖｏｌ．２」（平成２−３−１５発行）

Attorney, Agent or Firm:

Tsuyoshi Yoshii (1 person outside)

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