【発明の名称】タンパク質精製のための方法

Title:

【発明の名称】タンパク質精製のための方法

Document Type and Number:

Japanese Patent JPH04504720

Kind Code:

A

Abstract:

A process for the purification of proteins from solutions containing contaminants of similar net charge and molecular weight is provided, comprising contacting a solution containing the desired protein with an immobilized metal affinity chromatography resin in a buffer containing a low concentration of a weak ligand for the chelant of the resin. The adsorbed protein is then eluted using a buffer having a high concentration of the same weak ligand, e.g., Tris. Particularly preferred features employ agarose-iminodiacetic acid resins having copper cations and are especially useful in obtaining preparations of homogeneous, stable rsT4 proteins.

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Inventors:

Staples, Mark A.
Purglis, Christopher A.

Application Number:

JP50678890A

Publication Date:

August 20, 1992

Filing Date:

April 12, 1990

Export Citation:

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Assignee:

Biogen, Inc.

International Classes:

C07K14/00; B01D15/38; C07K1/00; C07K1/20; C07K1/22; C07K14/42; C07K14/52; C07K14/555; C07K14/575; C07K14/58; C07K14/61; C07K14/65; C07K14/705; C07K14/73; C07K14/76; C07K16/00; (IPC1-7): C07K3/00; C07K13/00; C07K15/14

Attorney, Agent or Firm:

Shusaku Yamamoto

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