Title:
【発明の名称】Qβレプリカーゼの精製法
Document Type and Number:
Japanese Patent JP3134934
Kind Code:
B2
Abstract:
A process of producing highly pure, Q Beta replicase having a high level of activity is described. The present process allows isolation of Q Beta replicase from recombinant bacteria containing a clone of a phage DNA encoding the 65,000 weight subunit of Q Beta replicase. The present process provides an efficient method for producing pure Q Beta replicase which can readily be scaled up to commercial production levels.
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| WO/1999/007837 | ISOLATION AND IDENTIFICATION OF NOVEL POLYMERASES |
| WO/1999/037753 | ENZYME CATALYZED THERAPEUTIC AGENTS |
Inventors:
De Francesco, Robert A.
Application Number:
JP50089091A
Publication Date:
February 13, 2001
Filing Date:
September 19, 1989
Export Citation:
Assignee:
Gene-Trak Systems
International Classes:
C12N9/12; C12Q1/68; (IPC1-7): C12N9/22
Domestic Patent References:
| JP6222724A | ||||
| JP6044915B2 |
Other References:
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ，ＵＳＡ ７２（１）ｐ．１６２−１６６（１９７５）
日本生化学会「生化学データブック▲ＩＩ▼」（東京化学同人）（１９８２．１０．１）ｐ．１１９０−１１９３
阿南功一他編「基礎生化学実験法２抽出・分離・精製」（丸善株式会社）（昭４９．９．３０）ｐ．３４８．３５３−３５４
日本生化学会「生化学データブック▲ＩＩ▼」（東京化学同人）（１９８２．１０．１）ｐ．１１９０−１１９３
阿南功一他編「基礎生化学実験法２抽出・分離・精製」（丸善株式会社）（昭４９．９．３０）ｐ．３４８．３５３−３５４
Attorney, Agent or Firm:
Heikichi Odashima