PURPOSE: To make it possible to decide the presence and quantity of analyzing molecule by measuring the concentration of the molecule from the increase (decrease) in the relative fluorescence (fluorescence strength) of fluorescent dye by using a polymer anion-exchange layer.
CONSTITUTION: The biosensor comprises an arbitrary transparent carrier, monomolecular or multilayer disposed thereon, fluorescent dye F1 as donator dye chemically bonded to the uppermost layer, and antibody as acceptor ion bonded to the uppermost layer, wherein the antibody can be bonded as analyzing molecule labeled by fluorescent dye F2. Fester energy dislocation between the dye F1 and the dyes F1, F2 is utilized to detect the analyzing molecule in liquid phase capable of labeling by the dye F2. In this case, one or more exchange layer of polymer anion or polymer cation is used as monomolecular or multilayer, and the concentration of the bonded molecule is measured as the function of the increase (decrease) in the relative fluorescence strength (fluorescence strength) of the dye F2 (F1) or the change of the ratio between the two strengths. Thus, the presence and quantity of' the molecule can be decided.
RUTOGAA HAIRIGAA
BOUDEBIIN FUAN RENTO
ARUNO BETSUKAA
