Title:
【発明の名称】組換えポリペプチドの製品およびその製造、単離および精製方法
Document Type and Number:
Japanese Patent JPH02500876
Kind Code:
A
Abstract:
Disclosed is a method for the isolation and purification of polypeptides expressed in host cells by recombinant DNA techniques. A fused polypeptide is produced containing a desired polypeptide fused to additional amino acids. The additional amino acids define a leader sequence having properties exploitable in purification, a hinge region, and a cleavage site. The hinge region is cysteine-free and has a secondary structure which serves to expose the cleavage site to a selected endopeptidase. The method of the invention involves the production of a fused polypeptide which may be efficiently isolated by exploiting the properties of the leader sequence, and then efficiently cleaved at the cleavage site in an appropriate aqueous environment by virtue inter alia of the influence of the hinge on the cleavage agent/cleavage site reaction.
Inventors:
James S. Haston
Charles M, Cohen
Keck, Peter Sea
Ruger, David Sea
Charet, mark f
Claire, Robert
Opperman, Harman
Ridge, Richard Jay
Charles M, Cohen
Keck, Peter Sea
Ruger, David Sea
Charet, mark f
Claire, Robert
Opperman, Harman
Ridge, Richard Jay
Application Number:
JP50316188A
Publication Date:
March 29, 1990
Filing Date:
March 04, 1988
Export Citation:
Assignee:
Creative Bio Marines Incorporated
International Classes:
A61K38/00; C07K14/00; C07K14/485; C07K14/495; C07K14/52; C07K14/555; C07K14/575; C07K14/585; C07K14/65; C12N15/09; C07K14/81; C07K19/00; C12N15/62; C12P21/00; C12P21/02; C12R1/19; (IPC1-7): A61K37/02; C07K7/10; C07K7/36; C07K13/00; C07K15/12; C07K99/46; C12N15/62; C12P21/00; C12P21/02
Attorney, Agent or Firm:
Kyozo Yuasa (4 outside)