NEW MATERIAL:Plasminogen activator KYM produced by the mutant KYM-A of human rhabdomyosarcoma cell. Appearance, white powder; molecular weight, two bands between 56,000 and 62,000 (nonreduced type) and between 32,000 and 36,000 (reduced type) by SDS-polyacrylamide gel electrophoresis; solubility, 50μg/ ml in water and salt solution, insoluble in ethanol and ether.
USE: Thrombolytic agent. It is a weakly basic glycoprotein having stronger fibrin- bonding capability than urokinase. It is prepared preferably in the form administrable by intravenous injection.
PREPARATION: The cell KYM-I separated from human rhabdomyosarcoma (presented by Dr. Morimasa Sekiguchi, Medical Science Laboratory, Tokyo University) is cultured, and the mutant KYM-A prepared by the repeated separation, selection and culture of the cell is subjected to the suspension culture under agitation to obtain the titled substance on an industrial scale.
YOSHIYAMA HIROSHI
ODA MUNEHIRO
| JPS5728009A | 1982-02-15 |