PURPOSE: To obtain the titled factor, by cultivating cells derived from human T lymphocytes in a culture medium containing a specific stimulating agent.
CONSTITUTION: Cells derived from human T lymphocytes, e.g. T lymphocytes derived from human peripheral blood, T lymphomatous cells, T leukemic cells or T cellular hybridized strains, are cultivated in a culture medium containing a polysaccharide, e.g. having (1→3) glucoside bonds in the main chain, or a bacterial cellular component, e.g. a bacterial cell of Streptococcus pyogenes, picibanil or cell wall skeletonic component of a bacterium of the genus Nocardia, having the immunological activating action as a stimulating agent to the aimed more selective colony stimulating factor (CFS) with little contamination by other lymphokines. The resultant CFS is useful as medicines for treating human hypogranulocytosis, etc. The above-mentioned CFS has 15,000W45,000 daltons molecular weight and is inactivated by treatment with trypsin, etc. without inactivation by treatment at 37°C for 2hr with deoxyribonucleases (DNAases) or ribonucleases (RNAases).
AKIYAMA YUKIO
KASHIMA SHINICHI
YOSHIMOTO RIYOUTA
HAMURO JIYUNJI
