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Title:
METHOD FOR SEPARATING AND PURIFYING HUMAN LACTOFERRIN FROM HUMAN MILK
Document Type and Number:
Japanese Patent JPS60166619
Kind Code:
A
Abstract:

PURPOSE: To separate and purify human lactoferrin in high yield and purity, from human milk, by using a specific affinity column.

CONSTITUTION: A monoclonal anti-human lactoferrin antibody produced by a hydridoma is immobilized to an insoluble carrier [e.g. Affigel-10(Biolad Corp.), etc.], and is used as an affinity column to separate and purify human lactoferrin from human milk. The above antibody can be prepared by fusing the spleen cell of mouse immunized with human lactoferrin and a myeloma cell, administering the resultant hybridoma to mouse, and recovering and purifying the ascites of the mouse.

EFFECT: The objective compound can be separated easily from the other milk components only by passing through a column once, without using pretreatment.

USE: Preventive and remedy for the infective diseases of infants caused by iron- demanding pathogen.


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Inventors:
KAWAKAMI HIROSHI
NIIMOTO YOUJI
DOUSAKO SHIYUNICHI
AHIKO KENKICHI
Application Number:
JP2241384A
Publication Date:
August 29, 1985
Filing Date:
February 09, 1984
Export Citation:
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Assignee:
SNOW BRAND MILK PRODUCTS CO LTD
International Classes:
A61K39/395; A61K35/20; A61K38/16; C07K1/16; C07K1/22; C07K14/435; C07K16/00; C12P21/08; C12R1/91; (IPC1-7): A61K35/20; A61K37/14; A61K39/395
Other References:
TRANSACTIONS,ILL.STATE ACAD.SCI.=1978
THE JOURNAL OF BIOLOGICAL CHEMISTRY=1981
THROMB HAEMOSTAS=1983
Attorney, Agent or Firm:
Kiyoya Fujino (1 outside)



 
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