Title:
TRANSCRIPTION-TRANSLATION CONJUGATE CELL-FREE PROTEIN SYNTHESIS
Document Type and Number:
Japanese Patent JP2017131171
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a cell-free protein synthesis technique with high protein expression efficiency.SOLUTION: The present invention provides use of ABPV(Acute bee paralysis virus) IGR(intergenic region)-IRES(internal ribosome entry sites) nucleic acid sequences for increasing protein expression levels in a transcription-translation conjugate cell-free protein synthesis system.SELECTED DRAWING: Figure 1
Inventors:
NAGAOKA JUNJI
Application Number:
JP2016015070A
Publication Date:
August 03, 2017
Filing Date:
January 29, 2016
Export Citation:
Assignee:
KYOTO INST TECH
International Classes:
C12N15/09; C12P21/02
Domestic Patent References:
| JP2003245094A | 2003-09-02 |
Other References:
BIOTECHNOL. BIOENG. (2014) VOL.111, NO.1, P.25-36, JPN6019041463, ISSN: 0004453030
NUCLEIC ACIDS RES. (2011) VOL.39, NO.16, P.7276-7288, JPN6019041464, ISSN: 0004453031
NUCLEIC ACIDS RES. (2011) VOL.39, NO.16, P.7276-7288, JPN6019041464, ISSN: 0004453031
Attorney, Agent or Firm:
Patent Business Corporation Saegusa International Patent Office