Title:
多剤排出蛋白質欠損株由来の形質転換株およびそれらを用いた微生物変換方法
Document Type and Number:
Japanese Patent JP5646168
Kind Code:
B2
Abstract:
Disclosed is a means for improving the poor conversion efficiency in a conventional bioconversion system using a transformant which is given by introducing a gene originated from xerogenic organisms. A transformant is prepared by using a host which is defective in a gene encoding a multidrug efflux protein and introducing a gene originated from xerogenic organisms. Use of the transformant results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound. In case, an Escherichia coli is used as the host, the gene encoding a multidrug efflux protein to be defective may be tolC, acrA, acrB and the like.
Inventors:
藤井 匡
落合 淳
伊藤 将士
株本 浩樹
藤井 良和
町田 和弘
落合 淳
伊藤 将士
株本 浩樹
藤井 良和
町田 和弘
Application Number:
JP2009501308A
Publication Date:
December 24, 2014
Filing Date:
February 28, 2008
Export Citation:
Assignee:
日本マイクロバイオファーマ株式会社
International Classes:
C12N1/21; C12P7/02; C12P33/06
Domestic Patent References:
| JP2005504530A | 2005-02-17 |
Foreign References:
| WO2007138894A1 | 2007-12-06 | |||
| WO2008096695A1 | 2008-08-14 |
Other References:
JPN6012055074; ＮＡＫＡＭＵＲＡ， Ｋ． ｅｔ ａｌ．: Ａｎｔｉｍｉｃｒｏｂ． Ａｇｅｎｔｓ Ｃｈｅｍｏｔｈｅｒ． Ｖｏｌ．４５， Ｎｏ．１２, 2001, ｐｐ．３３６６-３３７４
JPN6012055076; 新見昌一: Ｊｐｎ． Ｊ． Ｍｅｄ． Ｍｙｃｏｌ． Ｖｏｌ．４５, 2004, ｐｐ．６３-６９
JPN6012055072; ＳＲＩＫＵＭＡＲ， Ｒ． ｅｔ ａｌ．: Ａｎｔｉｍｉｃｒｏｂ． Ａｇｅｎｔｓ Ｃｈｅｍｏｔｈｅｒ． Ｖｏｌ．４２， Ｎｏ．１, 1998, ｐｐ．６５-７１
JPN6012055079; ＢＥＲＮＨＡＲＤＴ， Ｒ．: Ｊ． Ｂｉｏｔｅｃｈｎｏｌ． Ｖｏｌ．１２４, 2006, ｐｐ．１２８-１４５
JPN6012055082; ＳＡＷＡＤＡ， Ｎ． ｅｔ ａｌ．: Ｂｉｏｃｈｅｍ． Ｂｉｏｐｈｙｓ． Ｒｅｓ． Ｃｏｍｍｕｎ． Ｖｏｌ．３２０, 2004, ｐｐ．１５６-１６４
JPN6012055084; ＵＣＨＩＤＡ， Ｅ． ｅｔ ａｌ．: Ｂｉｏｃｈｅｍ． Ｂｉｏｐｈｙｓ． Ｒｅｓ． Ｃｏｍｍｕｎ． Ｖｏｌ．３２３, 2004, ｐｐ．５０５-５１１
JPN6014017287; 今井嘉郎， 鎌滝哲也: 蛋白質・核酸・酵素 Ｖｏｌ．４３， Ｎｏ．３, 1998, ｐｐ．２０３-２１５
JPN6014017288; ＡＧＥＭＡＴＳＵ， Ｈ． ｅｔ ａｌ．: Ｂｉｏｓｃｉ． Ｂｉｏｔｅｃｈｎｏｌ． Ｂｉｏｃｈｅｍ． Ｖｏｌ．７０， Ｎｏ．１, 2006, ｐｐ．３０７-３１１
JPN6014017290; ＦＵＪＩＩ， Ｔ．: 'Ｓｔｒｅｐｔｏｍｙｃｅｓ ｓｐ． ＴＭ-７ ｂｏｘＡ， ｂｏｘＢ ｇｅｎｅ ｆｏｒ ｃｙｔｏｃｈｒｏｍｅ Ｐ４５０ ｔｙｐｅ ｃｏｍｐａｃｔｉｎ ６ｂｅｔａ-ｈｙｄｒｏｘｙｌａｓｅ， ｆｅｒｒｅｄｏｘ' ＧｅｎＢａｎｋ Ａｃｃｅｓｓｉｏｎ ＡＢ１８０８４５ , 20060201
JPN6014017291; ＳＵＬＡＶＩＫ， Ｍ．Ｃ． ｅｔ ａｌ．: Ａｎｔｉｍｉｃｒｏｂ． Ａｇｅｎｔｓ Ｃｈｅｍｏｔｈｅｒ． Ｖｏｌ．４５， Ｎｏ．４, 2001, ｐｐ．１１２６-１１３６
JPN6014039899; ＮＥＬＳＯＮ， Ｄ．Ｒ． ｅｔ ａｌ．: Ｐｈａｒｍａｃｏｇｅｎｅｔｉｃｓ Ｖｏｌ．６, 1996, ｐｐ．１-４２
JPN6014039903; ＮＥＬＳＯＮ， Ｄ．: Ｃｙｔｏｃｈｒｏｍｅ Ｐ４５０ Ｈｏｍｅｐａｇｅ , 20140905
JPN6014039905; ＮＥＬＳＯＮ， Ｄ．Ｒ． ｅｔ ａｌ．: ＤＮＡ Ｃｅｌｌ Ｂｉｏｌ． Ｖｏｌ．１２， Ｎｏ．１, 1993, ｐｐ．１-５１
JPN6012055074; ＮＡＫＡＭＵＲＡ， Ｋ． ｅｔ ａｌ．: Ａｎｔｉｍｉｃｒｏｂ． Ａｇｅｎｔｓ Ｃｈｅｍｏｔｈｅｒ． Ｖｏｌ．４５， Ｎｏ．１２, 2001, ｐｐ．３３６６-３３７４
JPN6012055076; 新見昌一: Ｊｐｎ． Ｊ． Ｍｅｄ． Ｍｙｃｏｌ． Ｖｏｌ．４５, 2004, ｐｐ．６３-６９
JPN6012055076; 新見昌一: Ｊｐｎ． Ｊ． Ｍｅｄ． Ｍｙｃｏｌ． Ｖｏｌ．４５, 2004, ｐｐ．６３-６９
JPN6012055072; ＳＲＩＫＵＭＡＲ， Ｒ． ｅｔ ａｌ．: Ａｎｔｉｍｉｃｒｏｂ． Ａｇｅｎｔｓ Ｃｈｅｍｏｔｈｅｒ． Ｖｏｌ．４２， Ｎｏ．１, 1998, ｐｐ．６５-７１
JPN6012055079; ＢＥＲＮＨＡＲＤＴ， Ｒ．: Ｊ． Ｂｉｏｔｅｃｈｎｏｌ． Ｖｏｌ．１２４, 2006, ｐｐ．１２８-１４５
JPN6012055082; ＳＡＷＡＤＡ， Ｎ． ｅｔ ａｌ．: Ｂｉｏｃｈｅｍ． Ｂｉｏｐｈｙｓ． Ｒｅｓ． Ｃｏｍｍｕｎ． Ｖｏｌ．３２０, 2004, ｐｐ．１５６-１６４
JPN6012055084; ＵＣＨＩＤＡ， Ｅ． ｅｔ ａｌ．: Ｂｉｏｃｈｅｍ． Ｂｉｏｐｈｙｓ． Ｒｅｓ． Ｃｏｍｍｕｎ． Ｖｏｌ．３２３, 2004, ｐｐ．５０５-５１１
JPN6014017287; 今井嘉郎， 鎌滝哲也: 蛋白質・核酸・酵素 Ｖｏｌ．４３， Ｎｏ．３, 1998, ｐｐ．２０３-２１５
JPN6014017288; ＡＧＥＭＡＴＳＵ， Ｈ． ｅｔ ａｌ．: Ｂｉｏｓｃｉ． Ｂｉｏｔｅｃｈｎｏｌ． Ｂｉｏｃｈｅｍ． Ｖｏｌ．７０， Ｎｏ．１, 2006, ｐｐ．３０７-３１１
JPN6014017290; ＦＵＪＩＩ， Ｔ．: 'Ｓｔｒｅｐｔｏｍｙｃｅｓ ｓｐ． ＴＭ-７ ｂｏｘＡ， ｂｏｘＢ ｇｅｎｅ ｆｏｒ ｃｙｔｏｃｈｒｏｍｅ Ｐ４５０ ｔｙｐｅ ｃｏｍｐａｃｔｉｎ ６ｂｅｔａ-ｈｙｄｒｏｘｙｌａｓｅ， ｆｅｒｒｅｄｏｘ' ＧｅｎＢａｎｋ Ａｃｃｅｓｓｉｏｎ ＡＢ１８０８４５ , 20060201
JPN6014017291; ＳＵＬＡＶＩＫ， Ｍ．Ｃ． ｅｔ ａｌ．: Ａｎｔｉｍｉｃｒｏｂ． Ａｇｅｎｔｓ Ｃｈｅｍｏｔｈｅｒ． Ｖｏｌ．４５， Ｎｏ．４, 2001, ｐｐ．１１２６-１１３６
JPN6014039899; ＮＥＬＳＯＮ， Ｄ．Ｒ． ｅｔ ａｌ．: Ｐｈａｒｍａｃｏｇｅｎｅｔｉｃｓ Ｖｏｌ．６, 1996, ｐｐ．１-４２
JPN6014039903; ＮＥＬＳＯＮ， Ｄ．: Ｃｙｔｏｃｈｒｏｍｅ Ｐ４５０ Ｈｏｍｅｐａｇｅ , 20140905
JPN6014039905; ＮＥＬＳＯＮ， Ｄ．Ｒ． ｅｔ ａｌ．: ＤＮＡ Ｃｅｌｌ Ｂｉｏｌ． Ｖｏｌ．１２， Ｎｏ．１, 1993, ｐｐ．１-５１
JPN6012055074; ＮＡＫＡＭＵＲＡ， Ｋ． ｅｔ ａｌ．: Ａｎｔｉｍｉｃｒｏｂ． Ａｇｅｎｔｓ Ｃｈｅｍｏｔｈｅｒ． Ｖｏｌ．４５， Ｎｏ．１２, 2001, ｐｐ．３３６６-３３７４
JPN6012055076; 新見昌一: Ｊｐｎ． Ｊ． Ｍｅｄ． Ｍｙｃｏｌ． Ｖｏｌ．４５, 2004, ｐｐ．６３-６９
Attorney, Agent or Firm:
Patent business corporation patent firm Sykes