Title:
トリテルペン水酸化酵素
Document Type and Number:
Japanese Patent JP4668176
Kind Code:
B2
Abstract:
Soyasapogenol B is biosynthesized via two steps of hydroxylation reaction of its precursor ²-amyrin. However, the gene of the hydroxylase concerned in this reaction has not been revealed. Therefore, it was impossible to apply a genetic engineering technique on the hydroxylase. The present inventors reveals that a sequence which corresponds to a soybean-derived cytochrome P-450 gene CYP93E1 encodes an enzyme protein that carries out hydroxylation of the 24-position of an oleanane type triterpene, and also provides a method for applying said gene making use of a genetic engineering technique.
Inventors:
Hiroaki Hayashi
Kenichiro Inoue
Masaki Hoshino
Shibuya Masaaki
Yutaka Ebitsuka
Kenichiro Inoue
Masaki Hoshino
Shibuya Masaaki
Yutaka Ebitsuka
Application Number:
JP2006510327A
Publication Date:
April 13, 2011
Filing Date:
February 25, 2005
Export Citation:
Assignee:
Meiji Seika Co., Ltd.
International Classes:
C12N15/09; C12N1/19; C12N9/00; C12N9/02; C12N15/53; C12P15/00; C12P33/06
Foreign References:
| WO2002086142A1 | 2002-10-31 |
Attorney, Agent or Firm:
Shohei Oguri
Hironori Honda
Toshimitsu Ichikawa
Hironori Honda
Toshimitsu Ichikawa