PURPOSE: To achieve the miniaturization and wt. reduction of the title device by employing a small-size solenoid or a stepping motor type actuator because control can be carried out by small force by forming the first projection part to the tube pressing surface of an actuator so as to protrude toward an actuator receiver.

CONSTITUTION: The supply apparatus of a precipitant Pr as a particulate or liquid protein fractionating and separating agent provided with a tube clamp device is especially constituted of a column 20, a precipitant supply column 22 and the tube 24 mutually connecting the column 20 and the precipitant supply column 22 and guiding the precipitant Pr from the precipitant supply column 22 to the column 20. To the pressing surface 96a opposed to a receiving member 92 of a tube press member 96, the first projection part 96b formed so as to protrude toward the receiving member 96 is integrally provided. By this constitution, since no strong driving force is required, as an actuator, for example, a stepping motor can be used in place of a solenoid.