Title:
VARIANT HUMAN PROUROKINASE
Document Type and Number:
Japanese Patent JP3314398
Kind Code:
B2
Abstract:
PURPOSE: To enable an increase of half-life in blood and an improvement in thrombolytic ability by substituting a specific amino acid residue mainly in an EGF region of a human PUK which is a fibrinolytic enzyme.
CONSTITUTION: The objective variant human prourokinase is obtained by substituting Ser, Asn, Pro, Gly or Tyr in a region from Asn at the 10th residue to Gly at the 60th residue on the N-terminal side of a human prourokinase (human PUK) with Thr, Pro or Ala. Furthermore, a method for producing the human prourokinase characterized by culturing a host transformed with the variant human prourokinase and expressing the variant human prourokinase is provided.
Inventors:
Toshizumi Tanabe
Masanori Morita
Masaaki Hirose
Yasuo Amatsuji
Kenichi Yano
Masanori Morita
Masaaki Hirose
Yasuo Amatsuji
Kenichi Yano
Application Number:
JP3017892A
Publication Date:
August 12, 2002
Filing Date:
January 20, 1992
Export Citation:
Assignee:
Mitsubishi Pharma Corporation
International Classes:
C12N5/10; C12N9/72; C12N15/09; C12N15/58; C12R1/91; (IPC1-7): C12N9/72; C12N5/10; C12N15/09
Domestic Patent References:
| JP63146789A | ||||
| JP3504327A |
Attorney, Agent or Firm:
Hajime Takashima
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