A freeze preserving method of an organization of pluripotent stem cell origin

Japanese Patent JP6012164

B2

The present invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the following (1) to (3): (1) a first step of bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) a second step of maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and, (3) a third step of cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution in the second step. According to the method of the present invention, a stable preservation method of a tissue derived from a pluripotent stem cell can be provided.

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Satoru Ando
Nakano Tokushige
Yoshiki Sasai
Nagatsugu Genji

JP2011258208A

October 25, 2016

November 25, 2011

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Sumitomo Chemical Co., Ltd.
RIKEN

C12N1/04; C12N5/0735; C12N5/0775; C12N5/0789; C12N5/0797

JP2011036196A
JP2001247401A
JP2007105013A
JP2002325571A

WO2011055855A1
WO2005045007A1
WO1995007611A1
WO2011028524A1
WO2009051671A1

KASAI M, et al., Cryopreservation of animal and human embryos by vitrification,Reprod Biomed Online.
MIYAMOTO,H. et al,The effects of time of equilibration with cryoprotectants at 0°C prior to freezing on the survival,Experientia,1986年,Vol.42, No.7,p.815-816

Toru Nakayama
Toru Sakamoto

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