Title:
ゲノム編集
Document Type and Number:
Japanese Patent JP7187435
Kind Code:
B2
Abstract:
The invention relates to a method comprising a) providing a host cell said host cell comprising an episomal replicon, said episomal replicon comprising a donor nucleic acid sequence, said host cell further comprising a target nucleic acid, b) providing helper protein(s) capable of supporting nucleic acid recombination in said host cell c) providing helper protein(s) and/or RNAs capable of supporting nucleic acid excision in said host cell wherein said donor nucleic acid sequence comprises in order. 5′-homologous recombination sequence 1-sequence of interest-homologous recombination sequence 2-3′ wherein said sequence of interest comprises a positive selectable marker d) inducing excision of said donor nucleic acid sequence e) incubating to allow recombination between the excised donor nucleic acid and said target nucleic acid f) selecting for recombinants having incorporated said donor nucleic acid into said target nucleic acid. Also described are nucleic acids and cells.
Inventors:
Fredens Julius
One Kaihan
Chin Jason W.
One Kaihan
Chin Jason W.
Application Number:
JP2019502096A
Publication Date:
December 12, 2022
Filing Date:
July 27, 2017
Export Citation:
Assignee:
UNITED KINGDOM RESEARCH AND INNOVATION
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Foreign References:
| WO2014143381A1 |
Other References:
Nucleic Acids Res., 2010, Vol. 38, No. 6, e92
BMC Microbiol., 2009, Vol. 9, No. 252
Nat. Genet., 2005, Vol. 37, No. 3, pp. 311-319
Science, 2008, Vol. 319, pp. 1215-1220
Science, 2016, Vol. 351, No. 6280
BMC Microbiol., 2009, Vol. 9, No. 252
Nat. Genet., 2005, Vol. 37, No. 3, pp. 311-319
Science, 2008, Vol. 319, pp. 1215-1220
Science, 2016, Vol. 351, No. 6280
Attorney, Agent or Firm:
Hiroki Masaki
Yusaku Tokai
Horiuchi Makoto
Masako Yamauchi
Shuichi Sonomoto
Yusaku Tokai
Horiuchi Makoto
Masako Yamauchi
Shuichi Sonomoto