Title:
The immunogenicity bacteria small granule vesicle which has outer membrane protein
Document Type and Number:
Japanese Patent JP6077983
Kind Code:
B2
Abstract:
Knockout of the mltA gene gives bacteria that spontaneously release vesicles that are rich in immunogenic outer membrane proteins and that can elicit cross-protective antibody responses with higher bactericidal titres than OMVs prepared by normal production processes. Thus the invention provides a bacterium, such as an E.coli, having a knockout mutation of its mltA gene. The invention also provides a Escherichia bacterium, wherein the bacterium: (i) has a cell wall that includes peptidoglycan; and (ii) does not express a protein having the lytic transglycosylase activity of MItA protein. Tol-Pal knockouts of pathogenic E.coli are also disclosed. The invention also provides compositions comprising vesicles that, during culture of bacteria of the invention, are released into the culture medium.
Inventors:
Janet Addo-Bobier
Maria Grazia Pizza
Natalie Nore
Germano ferrari
Guido Grandy
Maria Grazia Pizza
Natalie Nore
Germano ferrari
Guido Grandy
Application Number:
JP2013246917A
Publication Date:
February 08, 2017
Filing Date:
November 29, 2013
Export Citation:
Assignee:
Novartis Vaxins and Diagnostics SRL
International Classes:
C12N1/21; A61K39/095; A61K39/108; A61P31/04; A61P37/04; C12N9/10; C12N15/09
Domestic Patent References:
| JP2004512269A | ||||
| JP2004527235A |
Other References:
INFECTION AND IMMUNITY,2004年 4月,vol.72 no.4,pp.1914-1919
The Journal of Infectious Diseases,2002年,vol.185,pp.220-228
The EMBO Journal,2001年,vol.20 no.24,pp.6937-6945
Eur. J. Biochem,2002年,vol.269,pp.3722-3731
The Journal of Infectious Diseases,2002年,vol.185,pp.220-228
The EMBO Journal,2001年,vol.20 no.24,pp.6937-6945
Eur. J. Biochem,2002年,vol.269,pp.3722-3731
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita