細胞を培養する改善された方法

Japanese Patent JP5448010

B2

The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.

WO/1995/032282	INTERLEUKIN-6 SPLICE VARIANT
JP2015508668	Sugarcane rod-shaped virus (SCBV) enhancer and its use in plant functional genomics
WO/2001/073032	COMPOSITIONS AND METHODS FOR THE THERAPY AND DIAGNOSIS OF PROSTATE CANCER

Zealstra, Helben, Mail
Hoff, Robert, Patrick
Sylder, Jacob

JP2012133156A

March 19, 2014

June 12, 2012

Click for automatic bibliography generation  

DSM IP ASSETS B.V.

C12N5/10; C07K1/34; C12M3/06; C12N5/071; C12N5/073; C12P21/00

JP2004528163A
JP7123972A
JP7298871A
JP2286072A
JP4293521A
JP2119772A
JP64085068A
JP63032484A
JP2002262855A
JP5031332A
JP10015357A
JP2138962A

US4806484
WO2005095578A1
WO2005010162A1
WO2004099396A1
US6544424

J.Clin.Pathol.,1990年,Vol.43,p.102-105
Journal of Immunological Methods,1995年,Vol.179,p.13-29
Journal of Immunological Methods,1996年,Vol.189,p.217-231
Biotechnology and Bioengineering,1990年,Vol.36,p.902-910

Ikeda adult
Kazuhiro Yamaguchi
Masakazu Noda
Yoshinori Shimizu

Previous Patent: JPS5448009

Next Patent: SUPPORTER FOR MOTOR