Title:
標的化したＤＮＡ配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体
Document Type and Number:
Japanese Patent JP6923205
Kind Code:
B2
Abstract:
The present invention provides a method of modifying a targeted site of a double stranded DNA of a monocot cell, comprising a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the given double stranded DNA and a nucleic acid base converting enzyme are bonded, with said double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into said targeted site, without cleaving at least one strand of said double stranded DNA in the targeted site, wherein the double stranded DNA is contacted with the complex by introducing a nucleic acid encoding the complex into the monocot cell. Furthermore, also provided is a complex used for the method, wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double stranded DNA of a monocot cell and a nucleic acid base converting enzyme are bonded.
Inventors:
Keiji Nishida
Zenpei Shimatani
Akihiko Kondo
Zenpei Shimatani
Akihiko Kondo
Application Number:
JP2017552750A
Publication Date:
August 18, 2021
Filing Date:
November 25, 2016
Export Citation:
Assignee:
National University Corporation Kobe University
International Classes:
C12N15/09; C07K19/00; C12N9/16; C12N9/78; C12N15/55; C12N15/62
Foreign References:
| WO2015133554A1 | ||||
| WO2014093595A1 | ||||
| WO2014093712A1 |
Other References:
OSAKABE, Y. et al.,Genome Editing with Engineered Nucleases in Plants,Plant Cell Physiol.,2015年 3月,vol.56, issue 3,p.389-440
MA, H. et al.,Multicolor CRISPR labeling of chromosomal loci in human cells,Proc. Natl. Acad. Sci. U. S. A.,2015年 3月10日,Vol. 112,pp. 3002-3007; Supporting Information
Farzadfard, F. et al.,Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas,ACS Synth. Biol.,2013年,Vol. 2,pp. 604-613
MA, H. et al.,Multicolor CRISPR labeling of chromosomal loci in human cells,Proc. Natl. Acad. Sci. U. S. A.,2015年 3月10日,Vol. 112,pp. 3002-3007; Supporting Information
Farzadfard, F. et al.,Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas,ACS Synth. Biol.,2013年,Vol. 2,pp. 604-613
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takeshi Komatani
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takeshi Komatani
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto