Title:
構造的にカロテノイドを過剰生産する選抜された細菌の菌株又はその突然変異体の発酵による高純度カロテノイドの製造方法
Document Type and Number:
Japanese Patent JP2010519927
Kind Code:
A
Abstract:
The present invention describes a process of production carotenoids in improved fermentation conditions of selected bacterial strains constitutively over-producing carotenoids or mutants thereof, purifying and isolating a specific crystalline carotenoid, preferably beta-carotene, for its use in the feed, food, cosmetic and pharmaceutical sectors. The present invention also describes a method for obtaining mutant strains constitutively overproducing carotenoids from naturally occurring bacterial strains, permitting the selection of mutants with high carotenoid yields and specificity towards a specific carotenoid. Additionally the invention describes the use of this method on obtained mutant strains for further improvement thereof. The present invention also describes said strains and improved conditions of fermentation for obtaining high concentrations of carotenoids and specificity towards a specific carotenoid, and further discloses purification steps, without cell disruption, for the extraction of carotenoids from the biomass.
Inventors:
Van Keuren, Frederick
Carolus, Ana Lucia
Lopez Burrito, Mafalda
Somer Ferreira, Bruno
Carolus, Ana Lucia
Lopez Burrito, Mafalda
Somer Ferreira, Bruno
Application Number:
JP2009552619A
Publication Date:
June 10, 2010
Filing Date:
March 08, 2007
Export Citation:
Assignee:
BIOTREND-INOVACAO E ENGENHARIA EM BIOTECNOLOGIA,SA
International Classes:
C12P23/00; C12N15/09
Other References:
JPN6012022759; Ｂｉｏｔｅｃｈｎｏｌ．Ｌｅｔｔ．，Ｖｏｌ．２６（２００４）ｐ．２５７-２６２
Attorney, Agent or Firm:
Creation International Patent Office