Title:
全身への送達を増加させる新規のリポソーム複合体
Document Type and Number:
Japanese Patent JP4203835
Kind Code:
B2
Abstract:
Highly efficient cationic liposomes have been developed as an improved delivery system for biologicallyactive reagents. A novel structure, the sandwich liposome, is formed and comprises one or more biologically active agents internalized between two bilomellar liposomes. This structure protects the incoming agent and accounts for the high efficiency of in vivo delivery and for the broad tissue distribution of the sandwich liposome complexes. These novel liposomes are also highly efficient carriers of nucleic acids. By using extruded DOTAP:cholesterol liposomes to form complexes with DNA encoding specific proteins, expression has been improved dramatically. Highest expression was achieved in the lung, while increased expression was detected in several organs and tissues.
Inventors:
Smith-Templeton, Nancy
Pavrakis, George N
Pavrakis, George N
Application Number:
JP51076598A
Publication Date:
January 07, 2009
Filing Date:
August 01, 1997
Export Citation:
Assignee:
THE UNITED STATES OF AMERICA
International Classes:
A61K9/127; A61K47/16; A61K47/28; A61K47/48; A61K48/00; C12N15/88
Other References:
STERNBERG, B. et al., morphology of cationic liposome/DNA complexes in relation to their chemical composition, JOURNAL OF LIPOSOME RESEARCH, 1996年 8月19日, vol. 6, no. 3, pages 515-533
KATO, T. et al., synthetic cationic amphiphile for liposome-mediated DNA transfection with less cytotoxicity, BIOL. PHARM. BULL, 1996年 6月, vol. 19, no. 6, pages 860-863
WASAN, E.K. et al., plasmid DNA is protected against ultrasonic cavitation-induced damage when complexed to cationic liposomes, JOURN. PHARM. SCIENCES, 1996年 4月, vol. 85, no. 4, pages 427-433
KATO, T. et al., synthetic cationic amphiphile for liposome-mediated DNA transfection with less cytotoxicity, BIOL. PHARM. BULL, 1996年 6月, vol. 19, no. 6, pages 860-863
WASAN, E.K. et al., plasmid DNA is protected against ultrasonic cavitation-induced damage when complexed to cationic liposomes, JOURN. PHARM. SCIENCES, 1996年 4月, vol. 85, no. 4, pages 427-433
Attorney, Agent or Firm:
Kazuo Shamoto
Shosuke Imai
Tadashi Masui
Tadahiko Kurita
Yasushi Kobayashi
Makoto Aihara
Shosuke Imai
Tadashi Masui
Tadahiko Kurita
Yasushi Kobayashi
Makoto Aihara