Title:
The primer set for bovine leukemia virus detection, and its use
Document Type and Number:
Japanese Patent JP6164590
Kind Code:
B2
Abstract:
A forward primer of a primer set in accordance with the present invention for detecting BLV is a mixture of (1) a first primer consisting of a polynucleotide including 15 or more successive bases including the 16th C in the base sequence of SEQ ID NO: 1 and (2) a second primer consisting of a plurality of kinds of polynucleotides including at least the first to 15th bases in the base sequence of SEQ ID NO: 2. Two or more of M, N, Y, K, and D which are included in the second primer are each a degenerate base which specifies two or more kinds of bases, and the second primer includes at least 10 kinds of polynucleotides including at least the first to 15th bases in the base sequences of SEQ ID NOs: 3 to 12.
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Inventors:
Yoko Ma
Shinnosuke Takeshima
Corner Tako Mayuko
Kawahara Mikiro
Saito
Yuri Muramatsu
Yume
Shinnosuke Takeshima
Corner Tako Mayuko
Kawahara Mikiro
Saito
Yuri Muramatsu
Yume
Application Number:
JP2014530562A
Publication Date:
July 19, 2017
Filing Date:
August 13, 2013
Export Citation:
Assignee:
RIKEN
RIKEN GENESIS CORPORATION
RIKEN GENESIS CORPORATION
International Classes:
C12N15/09; C12Q1/68
Foreign References:
| WO2012053666A1 |
Other References:
JIMBA M, et al.,BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm.,Retrovirology,2010年,Vol.7,Article No.91
Attorney, Agent or Firm:
Harakenzo world patent & trademark