Title:
A sweet taste receptor expression construct, a cell body which made this reveal, and its use
Document Type and Number:
Japanese Patent JP5905187
Kind Code:
B2
Abstract:
[Object] An object of the present invention is to provide a sweet taste receptor-expressing construct which can functionally stably express both of a sweet taste receptor (T1R2+T1R3) and a G protein ± subunit at a high expression efficacy, and a stable expression cell body expressing the construct. [Solution] The sweet taste receptor-expressing construct of the present invention is such that respective genes encoding sweet taste receptor subunits T1 R2 and T1R3, and a G protein ± subunit are inserted into the same plasmid. The cell body of the present invention is such that the sweet taste receptor-expressing construct of the present invention is gene-introduced into a 293 cell in which an FRT (Flippase Recognition Target) sequence is incorporated into one place in a genome, to express sweet taste receptor subunits T1 R2 and T1R3, and a G protein ± subunit simultaneously.
Inventors:
Keiko Abe
Takumi Misaka
Imada Takamasa
Satoshi Fujihara
Takumi Misaka
Imada Takamasa
Satoshi Fujihara
Application Number:
JP2009274976A
Publication Date:
April 20, 2016
Filing Date:
December 02, 2009
Export Citation:
Assignee:
National University Corporation Tokyo University
Hasegawa Fragrance Co., Ltd.
Hasegawa Fragrance Co., Ltd.
International Classes:
C12N15/09; C12N5/10; C12Q1/02
Other References:
今田隆将他,ヒト甘味受容体安定発現細胞株の作製の試み,日本農芸化学会2009年度(平成21年度)大会講演要旨集,2009年3月5日,p.232,3P0525A
今本文男他,生細胞への複数種cDNAの共導入と発現量の制御,蛋白質核酸酵素,2005年,Vol.50,No.13,p.1637-1648
Invitrogen 2004~2005年版カタログ,10-110~10-117
今本文男他,生細胞への複数種cDNAの共導入と発現量の制御,蛋白質核酸酵素,2005年,Vol.50,No.13,p.1637-1648
Invitrogen 2004~2005年版カタログ,10-110~10-117
Attorney, Agent or Firm:
Eiichiro Shimazaki