Title:
熱安定性ＣＡＳ９ヌクレアーゼ
Document Type and Number:
Japanese Patent JP7223377
Kind Code:
B2
Abstract:
Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.
Inventors:
Van der Oust, John
van kranenburg, richard
Bosma, Elecke Fenna
Mougiacos, Ioannis
Mohan Raju, Praltana
van kranenburg, richard
Bosma, Elecke Fenna
Mougiacos, Ioannis
Mohan Raju, Praltana
Application Number:
JP2019533103A
Publication Date:
February 16, 2023
Filing Date:
August 16, 2017
Export Citation:
Assignee:
Wageningen Universitet
Stitching Fall de Technish Wetenschapen
Stitching Fall de Technish Wetenschapen
International Classes:
C12N15/09; C12N5/10; C12N9/22; C12N15/11
Other References:
UniProtKB,[online],A0A178TEJ9_GEOSE,検索日[2020/12/01],2016年11月02日,
Molecular Cell,2016年04月07日,vol.62,p.137-147
Genome Biology,2015年,16:253,1-13
Molecular Cell,2016年04月07日,vol.62,p.137-147
Genome Biology,2015年,16:253,1-13
Attorney, Agent or Firm:
Hiroshi Kobayashi
Norio Omori
Fujita Nao
Norio Omori
Fujita Nao