10-DEAZAAMINOPTERIN: A NEW ARTHRITIS REMITTIVE DRUG.

Methotrexate is the only effective drug currently available in the classical antifolate series for the treatment of rheumatoid arthritis and related inflammatory disease such as asthma in humans. Methotrexate is a very toxic drug and the use of methotrexate in rheumatoid arthritis is limited by its toxicity. Many rheumatologists have concluded that toxicity is the major factor limiting prolonged MTX therapy. Toxic manifestations such as nausea, stomatitis, elevated liver function tests, cytopenias, and pulmonary toxicity have generally been reported in 30-60% of patients receiving the drug. A recent two year retrospective study documented that 93 % of patients experienced an adverse drug reaction during treatment.

10-deazaaminopterin is a more powerful antiproliferative agent than methotrexate; yet in clinical trials it was found to be remarkably less toxic to humans. In clinical trials as an anti-rheumatoid drug 10-deazaaminopterin was found to be superior to methotrexate. 10-deazaaminopterin can be conveniently prepared by the procedure of Nair as described in the Journal of Organic Chemistry (50:1875, 1985) as opposed to the more elaborate and expensive procedures using unstable intermediates for the preparation of methotrexate. Therefore, 10-deazaaminopterin can be considered as less toxic, more effective and an inexpensive anti-rheumatoid drug compared to methotrexate.

10-DEAZAAMINOPTERIN: A NEW RHEUMATOID ARTHRITIS REMITTIVE DRUG.

Background of the Invention

Rheumatoid arthritis is a chronic systemic disease believed to be of auto-immune origin. Common to all auto-immune diseases is the failure of the body's immune system to distinguish between self and non-self and to attack its own tissues as if belonging to a foreign organism. Severe articular pain and tissue destruction, leading to crippling joint deformities, as well as systemic manifestations such as vasculitis, heart disease, anemia, subcutaneous nodule formation, eye involvement, and others, are characteristic of this disease. There is no cure for rheumatoid arthritis. Drug therapy is aimed at reducing chronic inflammation and pain and at preventing progression of the disease. Aside from anti- inflammatory steroids, two classes of drugs are currently available for the treatment of this disease. One is made up by the non-steroidal anti-inflammatory drugs (NSAIDS) that help control inflammation and alleviate the pain and swelling of affected joints, but that have limited if any effect on the progression of the disease. Examples are aspirin, and a number of newer inhibitors of prostaglandin synthesis such as ibuprofen, naproxen, indomethacin, fenoprofen, sulindac, meclofenamate, and other related compounds. The second class is referred to as the disease modifying arthritis remittive drugs (DMARDs). Compounds in this class appear to arrest progression of the disease by mechanisms that remain largely unknown. Examples are gold salts, hydroxychloroquine, D-penicillamine, levamisol and cytotoxic agents, particularly cyclophosphamide and azathioprine. Antifolates, such as methrotrexate and sulfasalazine, belong in this latter group.

There is a general agreement that methotrexate is the most successful of the DMARDs. Recently it has been marketed in low dose tablets under the trade name Rheumatrex by the Lederle Co. specifically for the treatment of rheumatoid arthritis.

This invention relates to the use of 10-deazaaminopterin as a chemotiierapeutic agent for the

treatment of auto-immune disease such as rheumatoid arthritis in humans. 10-deazamminopterin in a non- trivial analogue of methotrexate and has the following structure.

10-deazaaminopterin differs from methotrexate with respect to the substitution at the 10t position. The N-methyl group in methotrexate is replaced by a methylene group in 10-deazaaminopterin. Methotrexate has the following structure.

10-DEAZA AMINOPTERIN

The first synthesis of 10-deazaaminopterin was reported by DeGraw, Kisliuk, Baugh and Nai in the Journal of Medicinal Chemistry (17:552, 1974).

In 1982 in the Journal of Medicinal Chemistry (25: 1227, 1982) DeGraw, Brown, Tagawa Kisliuk, Gaumont and Sirotnak published procedures for the synthesis of a number of 10-alkyl derivative of 10-deazaaminopterin. These 10-deazaaminopterin compounds were found to possess superior antitumo activity compared to methotrexate. Subsequently in 1983, a U.S. patent was granted to DeGraw an Sirotnak for 10-alkyl- 10-deazaaminopterins for possible use as anticancer drugs. (U.S. Patent 4.369319 1983).

In 1985, Nair in the Journal of Organic Chemistry (50: 1879, 1985) reported a convenient general procedure for the synthesis of both 10-deazaaminopterin and 10-alkyl-lO-deazaaminopterins. Results of anticancer clinical trials with 10-deazaaminopterin established that unlike methotrexate, 10- deazaaminopterin is significantly less toxic to cancer patients. These clinical trial summaries of 10- deazaaminopterin were reported in Cancer Treatment Reports (71 :95, 1987) by Thongprasert, Currie and

Budman. According to this study, nausea and vomiting were mild, and bone marrow depression was minimal.

The target enzyme of 10-deazaaminopterin is dihydrofolate reductase. Methotrexate and 10- deazaaminopterin inhibit this enzyme to approximately the same magnitude. In 1973 Baugh, Krumdieck and Nair in Biochemical and Biophysical Research Communication (52:27, 1973) and Nair and Baugh the same year in the Journal of Biochemistry (12:3923, 1974) reported the metabolism of methotrexate to its poly-gamma-glutamates. The role of these polyglutamyl metabolites in methotrexate toxicity has now been established. In 1988 Nair, Nanvati, Kumar, Gaumont and Kisliuk reported in the Journal of Medicinal Chemistry, that like methotrexate 10-deazaaminopterin is also metabolized to its poly-gamma- glutamates in mammalian tissues. (J. Med. Chem. 3_1 _. 181, 1988).

Since the mechanism of action of 10-deazaaminopterin is almost identical to that of the anti- rheumatoid drug methotrexate, and its documented lower toxicity to humans in anticancer clinical trials, in 1988 Nair, Krumdieck, Koopman, Alarcon and Castaneda selected 10-deazaaminopterin as a potential disease modifying rheumatoid remittive drug and a clinical trial was carried out to determine its efficacy in ameliorating rheumatoid arthritis in humans.

The results of a double blind clinical trial of 10-deazaaminopterin versus methotrexate are presented in the following tables and diagrams. As expected, 10-deazaaminopterin was shown to be equally effective as methotrexate and in certain clinical parameters such as swelling, pain and morning stiffness superior to methotrexate for the treatment of rheumatoid arthritis in humans.

10-Deazaaminopterin vs Methotrexate (MTX) in Rheumatoid Arthritis (RA)

The double-blind clinical trial of 10-deazaaminopterin versus methotrexate in rheumatoid arthritis was conducted at the Universidad Peruana Cayetano Heredia, Lima, Peru. In order to detect 30% differences in either direction for both-efficacy and toxicity a sample size of 20 was used. The trial was conducted according to the following protocol.

PROTOCOL

1) Objectives

The efficacy and toxicity of 10-deazaaminopterin vs methotrexate were compared in patients with RA (rheumatoid arthritis) who were eligible to receive methotrexate. The null hypotheses to be investigated included the following:

1. The efficacy of 10-deazaaminopterin is equal to that of methotrexate

2. The toxicity of 10-deazaaminopterin is equal to that of methotrexate Alternative hypotheses would be bidirectional (conceivable they could be unidirectional).

1. The efficacy of 10-deazaaminopterin is different from that of methotrexate (greater of lesser).

2. The toxicity of 10-deazaaminopterin is different from that of methotrexate (greater or lesser).

2) Subjects

Patients have failed at least 3 different nonsteroidal-anti-inflammatory drugs (NSAID) (aspirin included), and thus considered eligible for a remittive drug. They could have received (and failed) gold (oral/parenteral), d-penicillamine and/or antimalarials but they were off any of these drugs for a period of 3 months. The trial lasted 18 weeks.

3) Patient selection criteria were as follows:

Study patients were selected from those who had RA by the revised 1987 ARA criteria for no

less than six months, who had the onset of RA after age 16, were between 18 and 75 years of age, and have failed (lack of efficacy or toxicity) aspirin (to tolerance), or full doses of at lease 2 different non- steroidal anti-inflammatory drugs. Patients might have failed (toxicity or lack of efficacy) a remmittive drug (oral or parenteral gold, d-penicillamine, or antimalarials), with the exclusion of methotrexate, but not necessarily had to be on one. They were on an adequate method of contraception, if in their reproductive years. They had no hepatic or renal diseases (liver enzymes and creatine were within normal limits), no thrombocytopenia (platelets < 150,000 cells/mm ³ ), no leukopenia (< 3500 cells/mm ³ ) and no evidence of a malignancy or an active infectious process. In addition they had six or more swollen joints and two of the following criteria: 1) nine or more joints tender on pressure, 2) 45 minutes or more of morning stiffness, 3) a Westergren erythrocyte sedimentation rate ESR of 28min/hour or more.

The following medication restrictions were applied. Stable dose of NSAID, constant dose of prednisone not to exceed 10 mg of Prednisone (or equivalent) (stable for at least one month prior to study), no more man one intra-articular corticosteroid injection (this joint however would not be evaluated). Oxyphenylbutazone and phenylbutazone cannot be used; drugs used in the treatment of other chronic conditions (e.g. hypertension) would be allowed, but every effort was made to maintain them at a constant dose. Acetaminophen, Codeine or proproxyphene could be used during the trial for analgesia.

4) Study design

This study was an 18 week randomized double-blind trial of 10-deazaaminopterin vs methotrexate for the treatment of RA. Randomization was performed before the study began; an equal number of patients in each arm of the protocol was secured. Codes were made available to the investigator in sealed envelopes at the beginning of the trial; they were allowed to be opened by the investigator, only if felt to be mandatory for patient management. Patients received a three week supply of either 10- deazaaminopterin or methotrexate. Both 10-deazaaminopterin and methotrexate were given in physically indistinguishable 2.5 mg capsules. Initially the patients received three capsules/week (or 7.5 mg). The

capsules were taken at 12 hour intervals over a 24 hour period (8 a.m. - 8 p.m. - 8 a.m. the following day).

5) Evaluation

A data base which included a complete history, physical and laboratory exams was completed (Visit 0 or eligibility visit). After this visit was completed and before enrolling in the trial, patients received folic acid (lmg/day/7days) in order to bring all patient's folate to normal or above normal values and thus avoid toxicity to either 10-deazaaminopterin or methotrexate which could conceivably be worse in those patients who were folate deficient. If the patient was felt to be eligible, he/she was entered into the trial the following week. (Visit 1) at which time disease activity was assessed and a functional test completed. Patients returned to the clinic every three weeks at which time joint counts were preformed, side effects monitored and laboratory studies obtained. An assessment of response was done at week nine (Visit 3). At week 18 (Visit 6) or before (if the patient decided to be removed from the trial) the patient would have a complete exit evaluation, similar to the one performed on visit 1.

1.

Assessment included: Patients self assessment - scale of 1 - 5

Morning stiffness, in minutes on the day preceding the clinic visit. Physician's assessment - scale of 1 - 5

Joint counts for pain/tenderness in 60 diarthrodial joints and for swelling in 58 diarthrodial joints Laboratory tests included: CBC, with platelets and differential, Creatinine, SGOT and Alkaline phosphatase. Additionally the patients had a Westergren ESR at the baseline and at completion and a chest radiograph at baseline. 5 ml of sera were secured at entry and at study completion and stored at -20° C for further studies.

Adverse effects were determined by interviewing the patient; each new symptom developing since

the previous visit were recorded as to the date or onset, date of cessation, and whether it was felt to be related or unrelated to the study drug(s).

Minor itching and skin rashes were handled with antihistaminics or topical steroids; a drop in the WBC (OOOO/mm ³ ) or in the platelets (< 100,000/mm ³ ), or an increase in the liver enzymes (x2 normal), or an increase in the baseline creatinine (x 2 normal) would prompt holding the drug for one week; if the values improved, patients might be restarted on the drug; if no improvement occurred in 3 weeks, the patient was withdrawn. Likewise, any new symptom which could conceivably be due to these drugs would promptly hold the drug until the symptoms had either disappeared or improved significantly. If no improvement occurred in 3 weeks, the patient was withdrawn from the trial. If a patient developed a serious intercurrent infectious process, or required elective or emergency surgery during the study period, the patient was withdrawn from the trial.

6) Human Subjects

The study was conducted with approval of the Institutional Review Board at UPCH, Lima, Peru. Confidentiality was secured since data will be presented only in an aggregated fashion. The risks involved were the same (or maybe less) as those taking methotrexate. Methotrexate is currently used on the treatment of RA, either as a first or second line remittive drug in Lima, Peru.

8) TIMETABLE

6 OR W/D

History + Physical

Joint Evaluation

Chest X-ray

Hand/wrist films

Sera

Labs

ESR

RF

Adverse reaction

Check List

Functional test form X X

Administration of folic acid X

CLINICAL TRIAL SUMMARY

-DEAZAAMINOPTERIN ( -); METHOTREXATE ( ^■

10-Deazaaminopterin vs Methotrexate in Rheumatoid Arthritis

May 1989 Features of RA Patients

Duration of disease (mean, years) (Range 1-14) 4.6

Methotrexate

n = 7

10-Deazaaminopterin vs Methotrexate in Rheumatoid Arthritis Efficacy Evaluation

10-Deazaaminopterin Methotrexate

*60 joints for Pain/Swelling (0-3/joint), 58 joints for swelling (0-3/joint)

V ₃ = 3rd visit V ₆ = 6th visit

The results established that 10-deazaaminopterin is at least equally effective in all respects to methotrexate in the treatment of rheumatoid arthritis, and clearly superior to methotrexate in controlling pain, joint stiffness and in improving grip strength. Combined with the low frequency of toxicity and unexpectedly high attrition rate make 10-deazaaminopterin a superior drug than methotrexate in the treatment of rheumatoid arthritis in humans.

In accordance with the foregoing disclosure it has been determined that rheumatoid arthritis can be ameliorated in humans by the administration of 10-deazaaminopterin.

10-deazaaminopterin can be administered in humans by an available route including oral and parenteral. A dosage of 0.001 mg/kg to 1.0 mg/kg per day in humans should be sufficient to ameliorate rheumatoid arthritis. For ease of administration 10-deazaaminopterin can be provided in composition form or preferably dosage unit form. 10-deazaaminopterin can be administered in conjunction with a carrier or diluent which can be enclosed or encapsulated in a capsule, or take the form of tablets.