Title:
3D HUMAN LIVER ORGAN MODEL CONSTRUCTING METHOD, 3D HUMAN LIVER ORGAN MODEL AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/227243
Kind Code:
A1
Abstract:
Provided is a 3D human liver organ model constructing method, comprising: preparing human primary liver cells, or mixed cells of same and liver non-parenchymal cells, or human liver cancer cell lines into a single cell suspension, and mixing the single cell suspension with a matrix material to obtain a mixed cell suspension; inoculating the mixed cell suspension into cultivation micropores of a 3D organ-on-a-chip, and carry out cultivation at 37℃ to obtain a gelled 3D organ-on-a-chip; adding a culture medium into a liquid storage hole of the organ-on-a-chip, and carry out cultivation to obtain a 3D human liver organ model. Compared with other 2D human liver organ models, the constructed 3D human liver organ model has significantly enhanced response sensitivity to hepatotoxic drugs and shows stronger hepatotoxic damage effect for reported hepatotoxic drugs. Compared with an animal model, the 3D human liver organ model can effectively eliminate the screening difference caused by species difference.
Inventors:
XIAO RONGRONG (CN)
ZHOU YU (CN)
ZHOU YU (CN)
Application Number:
PCT/CN2020/102129
Publication Date:
November 18, 2021
Filing Date:
July 15, 2020
Export Citation:
Assignee:
BEIJING DAXIANG BIOTECH CO LTD (CN)
International Classes:
C12N5/074; A61L27/26; A61L27/38; C12N5/071; C12Q1/02
Foreign References:
| CN110951676A | 2020-04-03 | |||
| CN110272860A | 2019-09-24 | |||
| CN106916781A | 2017-07-04 | |||
| CN110249044A | 2019-09-17 |
Other References:
WANG YING: "Construction and Application of Liver/vessel Cell Chips Based on Nonswellable Hydrogel", MASTER THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, no. 3, 15 March 2020 (2020-03-15), CN , XP055867518, ISSN: 1674-0246
LU SIMING: "Development of A Biomimetic Liver Tumor-on-a Chip Model Based on Decellularized Liver Matrix for Toxicity Testing", MASTER THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, no. 3, 15 March 2020 (2020-03-15), CN , XP055867509, ISSN: 1674-0246
WANG GAOSHANG, JIA ZHIDONG;LI YANG;PENG QING;GAO YI: "Construction of 3D Co-Culture Model of Liver Tissue Mimicking Hepatic Plate Constructed by Alginate Hydrogel", CHONGQING MEDICAL JOURNAL, CHONGQING YIXUE, CN, vol. 46, no. 17, 30 June 2017 (2017-06-30), CN , pages 2305 - 2308, XP055867505, ISSN: 1671-8348, DOI: 10.3969/j.issn.1671-8348.2017.17.001
NUPURA S BHISE, VIJAYAN MANOHARAN, SOLANGE MASSA, ALI TAMAYOL, MASOUMEH GHADERI, MARIO MISCUGLIO, QI LANG, YU SHRIKE ZHANG, SU RYO: "A liver-on-a-chip platform with bioprinted hepatic spheroids", BIOFABRICATION, INSTITUTE OF PHYSICS PUBLISHING LTD., UK, vol. 8, no. 1, 1 March 2016 (2016-03-01), UK , pages 014101, XP055684669, ISSN: 1758-5082, DOI: 10.1088/1758-5090/8/1/014101
YOON NO DA, LEE KWANG-HO, LEE JAESEO, LEE SANG-HOON: "3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 15, no. 19, 1 January 2015 (2015-01-01), UK , pages 3822 - 3837, XP055799076, ISSN: 1473-0197, DOI: 10.1039/C5LC00611B
LIU YAN, QINGZHEN YANG, XIAOMING CHEN, FANGWEI GU, HUI ZHANG: "Fabrication of Organ-on-a-Chip by 3D Printing Technology", ZHONGGUO SHENGWU YIXUE GONGCHENG XUEBAO - CHINESE JOURNAL OF BIOMEDICAL ENGINEERING, ZHONGGUO SHENGWU YIXUE GONGCHENG XUEHUI, CHINA, vol. 39, no. 1, 29 February 2020 (2020-02-29), CHINA , pages 97 - 108, XP055867500, ISSN: 0258-8021, DOI: 10.3969/j.issn.0258-8021.2020.01.13
LU SIMING: "Development of A Biomimetic Liver Tumor-on-a Chip Model Based on Decellularized Liver Matrix for Toxicity Testing", MASTER THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, no. 3, 15 March 2020 (2020-03-15), CN , XP055867509, ISSN: 1674-0246
WANG GAOSHANG, JIA ZHIDONG;LI YANG;PENG QING;GAO YI: "Construction of 3D Co-Culture Model of Liver Tissue Mimicking Hepatic Plate Constructed by Alginate Hydrogel", CHONGQING MEDICAL JOURNAL, CHONGQING YIXUE, CN, vol. 46, no. 17, 30 June 2017 (2017-06-30), CN , pages 2305 - 2308, XP055867505, ISSN: 1671-8348, DOI: 10.3969/j.issn.1671-8348.2017.17.001
NUPURA S BHISE, VIJAYAN MANOHARAN, SOLANGE MASSA, ALI TAMAYOL, MASOUMEH GHADERI, MARIO MISCUGLIO, QI LANG, YU SHRIKE ZHANG, SU RYO: "A liver-on-a-chip platform with bioprinted hepatic spheroids", BIOFABRICATION, INSTITUTE OF PHYSICS PUBLISHING LTD., UK, vol. 8, no. 1, 1 March 2016 (2016-03-01), UK , pages 014101, XP055684669, ISSN: 1758-5082, DOI: 10.1088/1758-5090/8/1/014101
YOON NO DA, LEE KWANG-HO, LEE JAESEO, LEE SANG-HOON: "3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 15, no. 19, 1 January 2015 (2015-01-01), UK , pages 3822 - 3837, XP055799076, ISSN: 1473-0197, DOI: 10.1039/C5LC00611B
LIU YAN, QINGZHEN YANG, XIAOMING CHEN, FANGWEI GU, HUI ZHANG: "Fabrication of Organ-on-a-Chip by 3D Printing Technology", ZHONGGUO SHENGWU YIXUE GONGCHENG XUEBAO - CHINESE JOURNAL OF BIOMEDICAL ENGINEERING, ZHONGGUO SHENGWU YIXUE GONGCHENG XUEHUI, CHINA, vol. 39, no. 1, 29 February 2020 (2020-02-29), CHINA , pages 97 - 108, XP055867500, ISSN: 0258-8021, DOI: 10.3969/j.issn.0258-8021.2020.01.13
Attorney, Agent or Firm:
BEIJING KANGSHENG INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
Download PDF:
Previous Patent: LIQUID CRYSTAL DISPLAY PANEL
Next Patent: POWDER COMPOSITION AND PARTICLE SIZE CONTROL SYSTEM, AND CONTROL METHOD FOR SAME
Next Patent: POWDER COMPOSITION AND PARTICLE SIZE CONTROL SYSTEM, AND CONTROL METHOD FOR SAME