Title:
3'UTR CONSTRUCTION METHOD AND APPLICATION
Document Type and Number:
WIPO Patent Application WO/2022/166771
Kind Code:
A1
Abstract:
Provided are a 3'UTR polynucleotide capable of improving gene expression, a construction method therefor and an application thereof. The polynucleotide comprises a sequence after knocking out one or more microRNA recognition sites in a 3'UTR region of a PCBP4 gene or a PIWIL4 gene, or a 3'UTR sequence containing at least three tandem repeats of m6A site sequences and a linker sequence between any two repeat sequences.
Inventors:
LIU TAO (CN)
WANG MENG (CN)
GAO HAIXIA (CN)
SHAO MEIQI (CN)
JIANG TING (CN)
WANG PEI (CN)
QIAN QIJUN (CN)
WANG MENG (CN)
GAO HAIXIA (CN)
SHAO MEIQI (CN)
JIANG TING (CN)
WANG PEI (CN)
QIAN QIJUN (CN)
Application Number:
PCT/CN2022/074391
Publication Date:
August 11, 2022
Filing Date:
January 27, 2022
Export Citation:
Assignee:
MAXIRNA SHANGHAI PHARMACEUTICAL CO LTD (CN)
MAXIRNA ZHEJIANG TECH CO LTD (CN)
MAXIRNA ZHEJIANG TECH CO LTD (CN)
International Classes:
C07K14/47; C12N7/00; C12N15/10; C12N15/11; C12N15/67; C12Q1/68
Foreign References:
| US20190100761A1 | 2019-04-04 | |||
| US20190264214A1 | 2019-08-29 | |||
| US20180195077A1 | 2018-07-12 | |||
| CN107207557A | 2017-09-26 |
Other References:
GHINI FRANCESCO, RUBOLINO CARMELA, CLIMENT MONTSERRAT, SIMEONE INES, MARZI MATTEO J., NICASSIO FRANCESCO: "Endogenous transcripts control miRNA levels and activity in mammalian cells by target-directed miRNA degradation", NATURE COMMUNICATIONS, vol. 9, no. 1, 1 December 2018 (2018-12-01), XP055955752, DOI: 10.1038/s41467-018-05182-9
ZHENG JIAN, LIU XIAOBAI, WANG PING, XUE YIXUE, MA JUN, QU CHENGBIN, LIU YUNHUI: "RETRACTED: CRNDE Promotes Malignant Progression of Glioma by Attenuating miR-384/PIWIL4/STAT3 Axis", MOLECULAR THERAPY, ELSEVIER INC., US, vol. 24, no. 7, 1 July 2016 (2016-07-01), US , pages 1199 - 1215, XP055955754, ISSN: 1525-0016, DOI: 10.1038/mt.2016.71
ZHENG JIAN, LIU XIAOBAI, WANG PING, XUE YIXUE, MA JUN, QU CHENGBIN, LIU YUNHUI: "RETRACTED: CRNDE Promotes Malignant Progression of Glioma by Attenuating miR-384/PIWIL4/STAT3 Axis", MOLECULAR THERAPY, ELSEVIER INC., US, vol. 24, no. 7, 1 July 2016 (2016-07-01), US , pages 1199 - 1215, XP055955754, ISSN: 1525-0016, DOI: 10.1038/mt.2016.71
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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