4-SUBST1TUTED PHENOXYPHENYLACETIC ACID DERIVATIVES [0001J This application claims the benefit of United States provisional patent applications Serial No. 60/839,018 filed on August 21, 2006 and Serial No. 60/851 ,385 filed October 13, 2006, which are incorporated herein by reference in their entirety.

[0002) The present invention relates to novel compounds, to pharmaceutical compositions comprising the compounds, to a process for making the compounds and to the use of the compounds in therapy. More particularly it relates to certain 4-substituted phenoxyphenylaceϋc acid derivatives useful in the treatment and prevention of allergic diseases such as asthma, allergic rhinitis and atopic dermatitis and other inflammatory diseases mediated by prostaglandin D ₂ (PGD ₂ ). |0003] International patent application, publication number WO 2004/058164 discloses inter alia, certain 2-substitutcd phenoxyphcnylacetic acid derivatives that modulate the PGDj-sclective receptor CRTH2 (chemoattractant receptor-homologous molecule expressed on Th2 cells), now more commonly referred to as DP2. The compounds arc said to be useful in the treatment of immunologic diseases such as asthma and allergic inflammation.

[0004] Il has now been found that certain 4-substituted phenoxy phenyl acetic acid derivatives bearing a particular substituent meta to the acetic acid moiety arc DP2 receptor modulators. As used herein, the term "modulator" includes antagonists.

[0005) According to one aspect, the present invention provides a compound of general formula (1):

(I)

[0006] or a salt thereof, wherein: [0007] R' is Ar'-L'-W-lλ; |0008] L ² is -(CR ⁰ RV; [0009] W is -CONR ³⁸ - or -NR ^3b CO-; [0010] R ^3a and R ^3b are each H or methyl;

[0011] L ¹ is -(CR ⁸ RV, -(CH=CH)-, or -O(CR"R ^b ) provided that when W is -NR ³ CO- then L ¹ is not -(CH=CH)-; [0012] n and m are independently 0, 1 or 2;

2

[0013] each R\ R ^b , R ^c and R ^d is independently H, F, OH, methyl or cyclopropyl, or R° and R ^b or

R ^c and R ^d together with the carbon to which they are attached form a cyclopropyl ring;

[0014] Ar ¹ is phenyl or naphthyl, each of which is unsubstituted or substituted with one or more substituents selected independently from F, Cl, CN, CF ₃ , CHF ₂ , CH ₂ F, SF ₅ , methyl, ethyl, cyclopropyl, t-butyl or OMc, or Ar ¹ is 1,2,3,4-tctrahydronaphthyl which is unsubstituted or substituted by mcthoxy,

[0015] provided that when Ar ¹ is naphthyl or 1 ,2,3,4-tetrahydronaphthyl then n is 0;

[0016] R ² is H, Ci-C ₆ alkyl, a residue of an amino acid or dipeptide, or CHR ^e (CH ₂ ) _q R ^f ;

[0017] q is 1 to 6;

[0018] R ^c is H, methyl or ethyl;

[0019] R ^f is NR ⁸ R ^h in which R ⁸ and R ^h each independently represents a hydrogen atom or a Ci-

C4 alkyl group, or R ⁸ and R ^h together with the nitrogen atom to which they are attached form a 5-6 membered heterocyclic ring optionally containing a second ring heteroatom selected from N and O, wherein said heterocyclic ring is optionally substituted with one or more groups independently selected from Ci-Ce alkyl;

[0020] A is CN, CH ₂ NH ₂ , CH ₂ NR ^4o C(=O)R ^s , or CH ₂ NR ⁴¹¹ SO ₂ R ⁶ , Cl, OMc, ( MQalkyl, cyclopropyi, H, F, Br, CH ₂ NH(I -4C alkyl), CH ₂ N(I -4C alkyl) ₂₎ thienyl, or phenyl which is unsubstituted or substituted with SO ₂ Me;

[0021] R ⁴ " and R ^4b arc each H or methyl;

[0022] R ⁵ is Ci-C ₆ alkyl, CpC ₆ alkoxy, C ₃ -C ₆ cycloalkyl, hetAr ¹ , or Ar ² ;

[0023] R ⁶ is C ₁ -C ₆ alkyl, NH(C ₁ -C ₆ alkyl), N(C ₁ -C ₆ alkyl) ₂ , Ar ³ , or hetAr;

[0024) hetAr ¹ is a 6 membered hetcroaryl which is unsubstituted or substituted with one or more groups independently selected from a halogen atom and a group of formula -NR ^5a R ^5b in which each of R ^5a and R ^5b independently represents a hydrogen atom or a (1 -4C) alkyl group, or together with the nitrogen atom to which they are attached form a pyrrolidinyl, piperidinyl or morpholino group;

[0025J hetAr ² is a 5-6 membered heteroaryl which is unsubstituted or substituted with one or more groups independently selected from C ₁ -C4 alkyl;

[0026] Ar ² is phenyl which is unsubstituled or substituted with one or more groups independently selected from a halogen atom, CN, SF ₅ , cyclopropyl, a Ci-C ₄ alkyl group, a C ₁ -C4 alkoxy group and a fluoroCrC.4 alkyl group;

[00271 Ar ³ is as defined for Ar ² ;

[0028] R ⁷ and R ⁸ are independently H, methyl, or F;

[0029] R ⁹ is H or methyl; and

[0030] R ¹⁰ is H or F.

[0031) In certain embodiments, the compound of Formula I has the Formula Ia

Ia wherein:

[0032] R ¹ is Ar ¹ -L ¹ -W-L ² -;

[0033] L ² is -(CR ^c R ^d ) _mS

[0034] W is -CONR ^3β - or -NR ^3b CO-;

10035} R ³ " and R ^3b are each H or methyl;

[0036) L ¹ is -(CR ⁰ RV ₁ -(CH=CH)-, or -O(CR°R ^b ) provided that when W is -NR ³ CO- then L ¹ is not -(CH=CH)-;

[0037] n and m arc independently 0, 1 or 2;

[0038] each R ⁰ , R ^b , R ^c and R ^d is independently H, F, methyl or cyclopropyl, or R" and R ^b or R ^c and R ^d together with the carbon to which they are attached form a cyclopropyl ring; [0039] Ar ¹ is phenyl or naphthyl, each of which is unsubstituted or substituted with one or more substituents selected independently from F, Cl, CN, CF ₃ , CHF ₂ , CH ₂ F, SF ₅ , methyl, ethyl, cyclopropyl, provided that when Ar ¹ is naphthyl then n is 0;

[0040] R ² is H, Ci-C ₆ alkyl, a residue of an amino acid or dipeptide, or CHR ^c (CH ₂ ) _q R ^f ; [0041] q is 1 to 6; [0042] R ^c is H, methyl or ethyl;

|0043) R ^f is NR ^B R ^h in which R ⁸ and R ^h each independently represents a hydrogen atom or a Ci- C _A alkyl group, or R ^g and R ^h together with the nitrogen atom to which they are attached form a 5-6 membercd heterocyclic ring optionally containing a second ring heteroatom selected from N and O, wherein said heterocyclic ring is optionally substituted with one or more groups independently selected from CpCβ alkyl;

[0044) A is CN, CH ₂ NH ₂ , CH ₂ NR ^4a C(=O)R ⁵ , or CH ₂ NR^SO ₂ R ⁶ , Cl, OMe, ( l-4C)alkyl, cyclopropyl;

[0045] R ^4a and R ^4b arc each H or methyl;

[0046] R ⁵ is Ci-C ₆ alkyl, C ₁ -C ₆ alkoxy, C ₃ -C ₆ cycloalkyl, hctAr ¹ , or Ar ² ; 10047] R ⁶ is C ₁ -C ₆ alkyl, NH(C ₁ -C ₆ alkyl), N(C ₁ -C ₆ alkyi) ₂ , Ar ³ , or hetAr ² ;

4

[0048} hetAr ¹ is a 6 membered heteroaryl which is unsubstituted or substituted with one or more groups independently selected from a halogen atom and a group of formula -NR ^5a R ^5b in which each of R ^5a and R ^Sb independently represents a hydrogen atom or a (1-4C) alkyl group, or together with the nitrogen atom to which they are attached form a pyrrolidinyl, piperidinyl or morpholino group;

(0049] hctAr ² is a 5-6 membered heteroaryl which is unsubstituted or substituted with one or more groups independently selected from C ₁ -C4 alkyl;

[0050] Ar ² is phenyl which is unsubstituted or substituted with one or more groups independently selected from a halogen atom, CN, SF ₅ , cyclopropyl, a C ₁ -C ₄ alkyl group, a Ci-C ₄ alkoxy group and a fluoroCi-C4 alkyl group;

[00511 Ar ³ is as defined for Ar ² ; and

[0052] R ⁷ and R ⁸ are independently H, methyl.

[0053) Compounds according to the present invention have been found to be DF2 modulators and are useful in the treatment of immunologic diseases such as asthma and allergic inflammation.

|0054] It will be appreciated that certain compounds according to the invention may contain one or more centers of asymmetry and may therefore be prepared and isolated in a mixture of isomers such as a racemic mixture, or in an enantiomerically pure form.

[0055] It will further be appreciated that the compounds of formula (I) or their salts may be isolated in the form of solvates, and accordingly that any such solvate is included within the scope of the present invention.

[0056] The compounds of Formula I include pharmaceutically acceptable salts thereof. In addition, the compounds of Formula 1 also include other salts of such compounds which are not necessarily pharmaceutically acceptable salts, and which may be useful as intermediates for preparing and/or purifying compounds of Formula I and/or for separating cnantiomers of compounds of Formula I.

[0057] The term "halogen" as used herein includes F, Cl, Br and I.

[0058] The terms "CpC ₄ alkyl" and "C)-C ₆ alkyl" as used herein refer to a saturated linear or branchcd-chain monovalent hydrocarbon radical of one to four or one to six carbon atoms, respectively. Examples of alkyl groups include, but are not limited to, methyl, ethyl, 1-propyl,

2-propyl, 1-butyl, 2-mcthyl-l-propyl, 2-butyi, 2-ιnethyI-2-propyl, 2,2-dimethylpropyl, 1-pentyl,

2-pentyl, 3-pentyl, 2-methyl-2-butyl, 3-methy 1-2 -butyl, 3-methyI- 1-butyl, 2-methyl- 1-butyl, 1-hexyl,

2-hexyl, 3-hexyl, 2-methyl-2-pentyl, 3-methyl-2-penlyl, 4-methyl-2-pentyl, 3-methyl-3-pentyl,

2-methyi-3-pentyl, 2,3-diniethyl-2-buryl, and 3,3-dimethyl-2-butyl.

[0059] The term "heteroaryl" as used herein refers to a monovalent aromatic radical of a 5-, 6-, or 7-membercd ring. Examples of heteroaryl groups include, but are not limited to, pyridinyl,

5 imidazolyl, imidazopyridinyl, pyrimidinyl, pyrazolyl, triazolyl, pyrazinyl, tetrazolyl, furyl, thienyl, isoxazolyl, thiazolyl, oxazolyl, isothiazolyl, and pyrrolyl.

[0060] The term "fluoroCi-C ₄ alkyl" as used herein refers to a Ci-C ₄ alkyl group wherein one or more of the hydrogens is replaced by a fluorine atom. Examples include CF ₃ , CH ₂ F, CHF ₂ ,

CH ₂ CH ₂ F, CH ₂ CH ₂ F ₂ , CH ₂ CF ₃ , CH ₂ CH ₂ CH ₂ F, CH ₂ CH ₂ CHF ₂ , CH ₂ CH ₂ CF ₃ , CHF(CH ₃ J ₂ ,

CH ₂ CHF(CH ₃ ) _2j and (he like.

[0061] The term "Ci-C ₆ alkoxy" as used herein refers to a Ci-C ₆ alkyloxy group. Exemplary alkoxy groups include mcthoxy, ethoxy, propoxy, isopropoxy, butoxy. t-butoxy, and isobutoxy.

[0062] In one embodiment, W is -CONR ³ "-. An example of a particular value for K ^u is hydrogen. In one embodiment, W is -NR ^3h CO-. In one embodiment R ^3h is hydrogen. In another embodiment, R ^3b is methyl. Examples of particular values for W arc CONH, NHCO and N(CH ₃ )CO.

[0063] In one embodiment, L ¹ is -(CR ^a R ^b ) _n -. Examples of particular values for n are 0, 1 and 2.

[0064] In one embodiment, L ¹ is a bond.

[0065] In one embodiment, L ¹ is -(CR°R ^b ). In certain embodiments, R° and R ^b are hydrogen. In certain embodiments, R° is OH. In certain embodiments, R° and R ^b together with the carbon atom to which are attached form a cyclopropylidine ring.

[0066] In one embodiment, L ¹ is -(CR°R ^b ) ₂ . In certain embodiments, R" and R ^b are hydrogen.

In certain embodiments, R" and R ^b together with the carbon atom to which are attached form a cyclopropylidine ring. In certain embodiments, R" and R ^b are attached to the same carbon. In other embodiments, R" and R ^b arc attached to different carbon atoms.

[0067] Examples of particular values for L ¹ are a bond, -CH ₂ -, -CH ₂ CH ₂ - and cyclopropylidcneCH ₂ .

(0068] A further example of L ¹ includes CH(OH)CH ₂ .

[0069] Further examples of V include cyclopropylidine groups, which can be represented by the following structures:

X x

[0070] In one embodiment, L ¹ is -O(CR°R ^b )-. An exemplary embodiment is -OCH ₂ -.

[0071] Referring to L ² , examples of particular values for m are 0 and 1. Examples of particular values for L ² are a bond and -CH ₂ -.

[0072] In certain embodiments, the sum of m and n is 0, 1 and 2. Particular mention is made of compounds in which the sum of m and n is 0 or 2.

[00731 Examples of values for -L'-W-lλ include -CONH-, -CH ₂ CONH-, -CH ₂ CH ₂ CONH-,

-CONHCHr, -CH ₂ CONHCH ₂ -, -NHCO-, -CH ₂ NHCO-, -NHCOCH ₂ -= -CH ₂ CH ₂ NHCO-,

6

-CH ₂ NHCOCH ₂ -, -CH ₂ CH ₂ NHCOCH ₂ -, -CH ₂ N(CH ₃ )COCH ₂ -, cyclopropylidcncCH ₂ NHCO and

-CH ₂ ONHCO-.

|00741 Further examples of values for -L ¹ -W-L ² - include -CH(OH)CH ₂ NHCO- and

-cyclopropylidineNHCO-.

100751 Particular values of -L'-W-lA are -CONH-, -NHCO-, -CH ₂ NHCO-, -NHCOCH ₂ -,

-CH ₂ CH ₂ NHCO-, -CH ₂ NHCOCH ₂ -, -CH ₂ CH ₂ NHCOCH ₂ -, -CH ₂ N(CHj)COCH ₂ -, cyclopropylidcncCH ₂ NHCO, -CH(OH)CH ₂ NHCO- and -cyclopropylidineNHCO-.

(0076] In one embodiment, Ar ¹ is a naphthyl group or a phenyl group that is substituted by one or two substituents independently selected from F, Cl CF ₃ , OMe, Me, and t-Bu.

[0077] In one embodiment, Ar ¹ is a naphthyl group or a phenyl group that is unsubstituted or substituted by one or tvvo substituents selected independently from F, Cl and CF ₃ .

[0078] In one embodiment, Ar ¹ is a naphthyl group or a phenyl group that is substituted by one or two substituents independently selected from OMe, Me, and t-Bu.

[0079] In one embodiment, Ar' is 1,2,3,4-tetrahydronaphthyl which is unsubstituted or substituted with OMe. In a particular embodiment, A is selected from the structures:

[0080] Examples of particular values for Ar ¹ are naphthyl, phenyl, 4-fluorophenyl, 3,4- di fluorophenyl, 2-chlorophenyl, 3-chlorophenyl, 4-chlorophenyl, 3,4-dichlorophenyl, 4-chloro-3- fluorophcnyl, 3-chloro-4-fIuorophcnyl, 4-trifluoromethylphcnyl, 3-fluoro-4-trifluoromethylphenyl, 3-trifluoromethylphenyl, 2,6-dichlorophenyl, 2,4-dichlorophenyl, 3-methoxyphcnyl, 4-ιcrtbulylphenyl, 3-fluorophenyl, 4-methylphenyI, l,2,3,4-tetrahydronaphth-2-yl and 6-methoxy- l,2,3,4-tetrahydronaphth-2-yl.

[0081] In certain embodiments, Ar ¹ is selected from naphthyl, phenyl, 4-fluorophenyl, 3,4- di fluorophenyl, 2-chlorophcnyl, 3-chlorophenyl, 4-chlorophenyl, 3,4-dichlorophenyl, 4-chloro-3- fluorophcnyl, 3-chloro-4-fluorophenyl, 4-lrifluoromethylphenyl and 3-fluoro-4- tri fluoromethy (phenyl .

[0082] In certain embodiments, Ar ¹ is selected from 3-trifluoromethylphenyl, 2,6- dichlorophenyl, 2,4-dichlorophenyl, 3-methoxyphenyI, 4-methoxyphenyl, 4-tertbutylphenyl, 3-fluorophenyl and 4-methylphenyl, and l,2,3,4-tetrahydronaphth-2-yl and 6-methoxy- 1,2,3,4- tetrahydronaphth-2-yl. [0083] In one embodiment, A is CN. [0084] In one embodiment, A is H.

7

[0085) In one embodiment, A is selected from F, Br and Cl.

|0086] In one embodiment, A is selected from (l-4C)alkyl. Particular examples include methyl and ethyl.

[0087] In one embodiment, A is cyclopropyl.

[0088] In one embodiment, A is selected from CH ₂ NH ₂ , CH ₂ NH(I-C alkyl), and CH ₂ N(MC alkyl) ₂ . Particular examples include CH ₂ NH ₂ and CH ₂ NMe ₂ .

[0089] In one embodiment, A is thienyl. In a particular embodiment, A is 2-lhienyI.

[0090] In one embodiment, A is phenyl which is unsubstitutcd or substituted with SO ₂ Me.

Particular examples include phenyl, 3-mcthylsulfonyIphenyI, and 4-mcthylsulfonylphenyl.

[0091] In one embodiment, A is C H ₂ N R ⁴¹¹ C( ³ O)R ⁵ . An example of a particular value for R ⁴ * is hydrogen. In one embodiment, R ⁵ is hetAr ¹ . An example of a particular value for a heteroaryl group represented by hetAr ¹ is a pyridyl group. Examples of optional subsϋtucnts on the heteroaryl group are NH ₂ , Cl, and pyrrolidinyl.

[0092] In another embodiment, R ⁵ is Ci-Ce alkyl; CpC ₆ alkoxy; C ₃ -C ₆ cycloalkyl; pyridyl which is unsubstituted or substituted by a halogen atom or a group of formula -NR ⁵ "R ^Sh in which each of

R ⁵ " and R ^5b independently represents a hydrogen atom or a (1 -4C) alkyl group, or together with the nitrogen atom to which they are attached form a pyrrolidinyl, piperidinyl or morpholino group; or a phenyl group that is unsubstiluted or substituted by one or two halogen atoms.

[0093] Examples of particular values for R ⁵ are methyl, mclhoxy, cyclohcxyl, pyrid-2-yl, pyrid-

3-yl, pyrid-4-yl, 6-chloro-pyrid-3-yl, 6-amino-pyrid-3-yl, 6-pyrrolidin-l-ylpyrid-3-yl or 4- fluorophenyl. An additional example of R ⁵ is 6-dimethylaminopyrid-3-yl.

|0094] In one embodiment, A is CH ₂ NR*SO ₂ R ^δ . An example of a particular value for R ^4b is hydrogen. In one embodiment, K ⁶ is hetAr ² . Examples of particular values for a heteroaryl group represented by hetAr arc an imidazolyl and a pyridyl group. Examples for optional substituents on the heteroaryl group are Ci -C ₄ alkyl, for example methyl.

[0095] In another embodiment, R ⁶ is Ci-C ₆ alkyl, NH(Ci-C ₆ alkyl), N(Ci-C ₆ alkyl) ₂ , a phenyl group that is unsubstituted or substituted by one or two halogen atoms, pyridyl or imidazolyl that is unsubstituled or substituted with a C1-C ₃ alkyl group.

[0096J Examples of particular values for R ⁶ arc methyl, dimethylamino, 4-fluorophenyl, 2,4- dichlorophenyl, pyrid-3-yl and l-methylimidazol-5-yl. An additional example of R ⁶ is pyrid-4-yl.

[0097] Examples of particular values for A are acetamidomcthyl, cyclohexylamidomethyl, methoxycarbonylaminomcthyl, picolinamidomethyl, nicotinamidomethyl, isonicotinamidomethyl, 6- chloropyrid-3-ylamidomethyl, 6-aminopyrid-3-ylamidomethyl, 6-pyrroIidin-l-ylpyrid-3- ylamidomelhyl, 4-fluorobenzamidometh\i, methylsulfonamidomethyl, N,N-

dimcthylsulfamoylamino, 4-fluorophcnylsulfonamidomethyl, 2,4-dichlorophenyl- sulfonamidomcthyl, l-methylimidazol-5-ylsulfonamidomethyl and pyrid-3-ylsulfonamidomethyl, which can be represented by the following structures, respectively:

(0098J Particular mention is made of acetamidomethyl, cyclohexylamidomethyl, methoxycarbonylaminomethyl, picolinamidomethyl, nicotinamidomethyl, isonicotinamidomethyl, 4- fluorobenzamidomethyl, methylsulfonamidomethyl, N,N-dimethylsulfamoylamino, 4- fluorophenylsulfonamidomethyl, 2,4-dichlorophenylsulfonamidomcthyl and pyrid-3- ylsulfonamidomethyl.

[0099J Particular values for A also include 6-dimeuiylamtnopyrid-3-ylamidomethyI, 2-(4-fluorophenylsulfonamido)acetamidomethyl, dimethylaminomethyl and (N- methylmethylsulfonamido)methyl, which can be represented by the following structures, respectively:

(00100) Examples of particular values for R ² when it represents a CpC ₆ alkyl group are methyl, ethyl, propyl, isopropyl and t-butyl.

(00101) In one embodiment, R ² is CHR ^c (CH ₂ ) _q R ^r . Examples of values for R ^c are hydrogen and methyl. In one embodiment, R ^r is di(l-4C)alkylamino, morpholino, or piperazinyl optionally substituted with (l-4C)alkyl. Examples of particular values for R ^r are dimethylamino, diethylamino, morpholino, piperazinyl and 1-mcthylpiperazinyl. Additional examples include NH ₂ and NHMe.

[00102| Examples of particular values for R ² when it represents CHR ^c (CH ₂ ) _q R ^f are:

[00103] In one embodiment, R ² is hydrogen.

(00104] On one embodiment, both R ⁷ and R ^s are H. In certain embodiments, R ⁷ is H and R ⁸ is methyl. In other embodiments, each of R ⁷ and R ^s is methyl.

[00105J According to another aspect, the present invention provides a process for the preparation a compound of formula (I) or a salt thereof as defined hcreinabove, which comprises:

[00106J (a) for a compound of formula (I) in which A is CN, R ⁷ and R ⁸ are independently H or Me, and R ¹⁰ is H or F ₃ reacting a corresponding compound having the formula:

in which P ¹ represents a hydrogen atom or a carboxyl protecting group and Z ¹ represents a leaving atom or group, with a corresponding compound having the formula

in which R ^l0 ° is H or F in the presence of a base; or

[00107] (b) for a compound of formula (I) in which A is -CH ₂ NH ₂ , R ¹⁰ is H, and R ⁷ and

R ⁸ are independently H or Me, reducing a corresponding compound formula (IV)

10

(IV) in which P ² is as defined for P"; or

[OOIOS] (c) for a compound of formula (I) in which A is -CH ₂ NH ₂ , R ⁷ and R ⁸ are independently H or Me, and R ¹⁰ is H, cleaving a corresponding compound of formula (V)

(V) in which P ³ is as defined for P ¹ ; or

(00109| (d) for a compound of formula (I) in which A is CH ₂ NR ⁴ C(=O)R ³ or CH ₂ NR ⁴ SO ₂ R ⁶ , R ⁷ and R ⁸ are independently H or Me, and R ¹⁰ is H, reacting a corresponding compound of formula (VI)

(VI) in which P ⁴ is as defined for P ¹ ; with a compound of formula R ³ COZ ² or R ⁶ SOjZ ³ , respectively, in which Z ² and Z ³ each represents a leaving atom or group; or

[00110] (e) for a compound of formula (I) in which R ⁷ and R ⁸ are independently H or Me, and R ¹⁰ is H, coupling a compound of formula (VII)

1 1

(VII) in which P ⁵ is as defined for P ¹ , A ² represents A or a protected form thereof and R ^la represents H-X ⁰ - L ² - in which X ⁰ represents HN or OC(=O), or a reactive derivative thereof; with a compound of formula (VHI)

Ar'-L'-XNH

(VlII) in which X ^b represents C(=O)O or NH, or a reactive derivative thereof; or [00111) (0 for a compound of formula (I) in which A is H, F or Cl, R ⁷ and R ⁸ are independently H or Me, and R ¹⁰ is H, coupling a corresponding compound having the formula OX)

(IX) in which A ³ is H, F or Cl, and P ⁶ is as defined for P ¹ , with a corresponding compound having the formula (X)

wherein E is an electron withdrawing group, in the presence of a base; and if desired removing said electron withdrawing group; or

|00112] (g) for a compound of formula (I) in which A is OMe or (l-4C)alkyI, R ⁷ and R ⁸ are independently H or Me, and R ¹⁰ is H, coupling a corresponding compound having the formula (XI)

12

(XI) in which A ⁴ is OMc or (l-4C)alkyl, respectively, and P ⁷ is as defined for P ¹ , with a corresponding compound having the formula (XII)

in the presence of a base, in which Z ⁴ represents a leaving atom or group, and R ^lx represents an electron withdrawing group convertible into a group R ¹ ; or

[00113] (h) for a compound of formula (I) in which A is Br or cyclopropyl, R ⁷ and R ⁸ are

H, and R . 10 is H, coupling a corresponding compound having the formula (XIV)

in which Z ⁵ is a leaving group or atom, with a compound having the formula (XV)

in the presence of a base, followed by converting the carbonyl group to a carboxyl group; or [00114) (i) for a compound of formula (I) in which A is methyl, thienyl, phenyl, or phenyl substituted with SO ₂ Me, R ⁹ is H. R ⁷ and R ⁸ are independently H or Me, and R ¹⁰ is H, reacting a corresponding compound having the formula (XVI)

13

(XVI) with a compound having the formula A ⁵ -ZnX, in the presence of a palladium (O) catalyst, or with a compound having the formula A ⁵ B(OH) ₂ in the presence of a base and a palladium (O) catalyst, where A ⁵ is methyl, Ihienyl, phenyl, or phenyl substituted with SC^Me and X is a halide; or [00115] (j) for a compound of formula O) in which R ⁷ is F, R ⁸ is H, and R ¹⁰ is H, treating a corresponding compound having the formula (XVII)

(XVII) with hydrogen fluoride;

(00116| (k) for a compound of Formula (I) in which A is CH ₂ NH(I -4C alkyl) or , CH ₂ N(I - 4C alkyl) _2s R ⁷ and R ⁸ are independently H or Me, and R ¹⁰ is H, reacting a corresponding compound having the formula

14 with an aldehyde having the formula HC(O)(I -4C alkyl); and removing any protecting group or groups and, if desired, forming a salt.

(001171 Referring to process (a), the leaving atom or group represented by Z ¹ may be, for example, a halogen atom such as a fluorine atom. The carboxyl protecting group may be any convenient carboxyl protecting group, for example as described in Greene & Wuts, eds., "Protecting

Groups in Organic Synthesis", John Wiley & Sons, Inc. Examples of carboxyl protecting groups include (l-6C)alkyl groups, such as methyl, ethyl and t-butyl. The base may be, for example, an alkali metal hydride or carbonate, such as sodium hydride, sodium carbonate or potassium carbonate, or a tertian' amine, such as triethylamine or N,N-diisopropylethylamine. Convenient solvents include amides, sulfoxides and nitriles, such as DMF, DMSO or acetonitrile. The reaction can be performed at an elevated temperature, such as in the range of from 50 to 150 ⁰ C.

(00118 J Compounds of formula (II) arc known or can be prepared from the corresponding 3-halo compound, such as a 3-bromo compound, by treatment with CuCN.

[00119] Referring to process (b), the compound of formula (IV) can be reduced by hydrogenation in the presence of a Group VIII metal catalyst, such as Rancy Ni with mcthanol/ammonia. The reaction can be conducted at a temperature in the range of from 0 to 100 ⁰ C.

[00120] Referring to process (c), the dioxoisoindolinyl group can be cleaved using HBr and acetic acid or hydrazine.

[00121] Compounds of formula (V) can be prepared by reacting a compound of formula (XIII)

(XIII) with a compound of formula (XIl)

(XII) in the presence of a base, in which Z ⁴ represents a leaving atom or group, such as a fluorine atom, and R ^lx represents an electron withdrawing group convertible into a group R ¹ , for example a nitro group that can be reduced to an amino group and then acylated. The base may be, for example, an

15 alkali metal hydride or carbonate, such as sodium hydride, sodium carbonate or potassium carbonate.

|00122] Compounds of formula (IX) can be prepared by reacting 4-hydroxyphenylacetic acid with 2-(hydroxymethyl)isoindoline-l,3-dione in the presence of a sulfonic acid, such as methancsulfonic acid, and followed if desired by introducing a protecting group P ³ .

[00123] Referring to process (d), the leaving atom or group represented by Z ² and Z ³ may be, for example, a halogen atom such as a fluorine atom. The reaction can be performed in the presence of a base, for example a tertiary amine such as diisopropylethylamine or pyridine. Convenient solvents include halogcnated hydrocarbons, such as methylene chloride. The reaction can be conducted at a temperature in the range of from 0 to 100 ⁰ C.

[00124| Referring to process (e), the coupling of the compound of formula (VII) with a compound of formula (VlII) may be performed using conventional amide bond formation conditions, for example by reacting an amine with a reactive derivative of a carboxylic acid, for example an acid halidc, such as an acid chloride. An example of A ¹ when it represents a protected form of A is a group of formula -CH ₂ NR ⁴ P ⁶ in which P ⁶ represents an amine protecting group. The amine protecting group may be any convenient amine protecting group, for example as described in

Greene & Wuts, eds., "Protecting Groups in Organic Synthesis", JoIm Wiley & Sons, Inc. Examples of amine protecting groups include acyl and alkoxycarbonyl groups, such as t-butoxycarbonyl

(BOC).

[00125] Referring to process (f), examples of electron withdrawing groups include NO ₂ . In embodiments wherein the electron withdrawing group is NOa, this group can be removed, if desired, by reducing the nitro group to an amino group using any convenient reducing conditions (for example, Zn and NH ₄ Cl) followed by cleavage of the amino group (for example, by treating the amino compound with isobutyl nitrite).

[00126] Referring to process (g), the base may be, for example, an alkali metal hydride or carbonate, such as sodium hydride, sodium carbonate or potassium carbonate. The Z ⁴ group represents a suitable leaving atom or group, such as a fluorine atom, and R ^lx represents an electron withdrawing group convertible into a group R ¹ , for example a nitro group that can be reduced to an amino group and then acylaled.

[00127] Referring to process (h), the leaving atom or group represented by Z ⁵ may be, for example, a halogen atom such as a fluorine atom. The base may be, for example, an alkali metal hydride or carbonate, such as sodium hydride, sodium carbonate, or potassium carbonate.

Convenient solvents include sulfoxides such as DMSO. The reaction can be performed at elevated temperatures, ibr example in the range of 50- 100 "C, for example at 85 ⁰ C. The carbonyl group can

16 be converted to a carboxyl group by treating the coupling product with methylsulfonyl/methylthiomcthane to provide an intermediate having the formula (XVIII)

(XVIII) followed by converting the intermediate (XVIlI) to the corresponding methyl ester upon treatment with HCI in methanol. The ester can be converted to the corresponding acid under standard hydrolysis conditions.

|00128) Referring to process (i), the halide represented by X can be F, Cl or Br. Convenient solvents include ethers such as THF or dioxanc. Suitable palladium (0) catalysts include Pd(PPIb) ₄ and bis(tri-t-butylphosphinc)palladium (0). A convenient base when compound (XVI) is reacted with a boronic acid reagent includes DBU. The reaction can be performed at elevated temperatures, for example in the range of 50-100 ⁰ C, for example 60 ⁰ C.

(00129] Referring to process (j), the reaction is conveniently performed in the presence of an amine base, for example pyridine.

[00130] Referring to process (k), the reaction is conveniently performed in the presence of a base, for example a hydride such as sodium cyanoborohydride in an alcohol solvent such as methanol. The reaction is preferably performed in the presence of a catalytic amount of acid, for example acetic acid. The reaction is conveniently performed at ambient temperature. [00131] The ability of test compounds to act as DP2 receptor modulators may be demonstrated by the assay described in Example A.

[00132] Compounds which are modulators of DP2 are useful in the treatment of diseases or disorders mediated by PGD ₂ , for example, diseases or disorders associated with overproduction or dysregulation Of PGD ₂ .

[00133] As used herein, the term treatment includes prophylaxis as well as treatment of an existing condition.

[00134] Examples of disorders or diseases that may be treated with compounds according to the invention include immunologic diseases.

17

[00135| Examples of immunologic diseases include allergic inflammatory diseases, such as asthma, atopic dermatitis, allergic rhinitis, seasonal allergies, food allergies, contact hypersensitivity (e.g., nickel sensitivity), hypcr-cosinophilic syndromes, and allergic conjunctivitis. [00136) Additional diseases or disorders which may be treated with the compounds of this invention include inflammatory bowel diseases such as Crohn's disease, ulcerative colitis, ileitis and enteritis, vasculitis, Behcet's syndrome, psoriasis and inflammatory dermatoses such as dermatitis, eczema, urticaria, viral cutaneous pathologies such as those derived from human papillomavirus, HIV or RLV infection, bacterial, fungal and other parasital cutaneous pathologies, and cutaneous lupus erythematosus, respiratory allergic diseases such as persensitivity lung diseases, chronic obstructive pulmonary disease and the like, autoimmune diseases, such as arthritis (including rheumatoid and psoriatic), systemic lupus erythematosus, type I diabetes, myasthenia gravis, multiple sclerosis, Graves' disease, glomerulonephritis and the like, graft rejection (including allograft rejection and graft-v-host disease), e.g., skin graft rejection, solid organ transplant rejection, bone marrow transplant rejection, fever, cardiovascular disorders such as acute heart failure, hypotension, hypertension, angina pectoris, myocardial infarction, cardiomyopathy, congestive heart failure, atherosclerosis, coronary artery disease, restenosis, thrombosis and vascular stenosis, cerebrovascular disorders such as traumatic brain injury, stroke, ischemic rcperfusion injury and aneurysm, cancers of the breast, skin, prostate, cervix, uterus, ovary, testes, bladder, lung, liver, larynx, oral cavity, colon and gastrointestinal tract (e.g., esophagus, stomach, pancreas), brain, thyroid, blood and lymphatic system, fibrosis, connective tissue disease and sarcoidosis, genital and reproductive conditions such as erectile dysfunction, gastrointestinal disorders such as gastritis, ulcers, nausea, pancreatitis and vomiting; neurologic disorders, such as Alzheimer's disease, sleep disorders such as insomnia, narcolepsy, sleep apnea syndrome and Pickwick Syndrome, pain, renal disorders, ocular disorders such as glaucoma, infectious diseases, viral infections such as HIV, and bacterial infections such as sepsis, inflammation, flushing, nasal congestion, and otitis media. [00137J Accordingly, another aspect of this invention provides a method of treating diseases or medical conditions in a mammal mediated by PGD2, comprising administering to said mammal one or more compounds of Formula I or a pharmaceutically acceptable salt or prodrug thereof in an amount effective to treat or prevent said disorder.

(00138] The phrase "effective amount" means an amount of compound that, when administered to a mammal in need of such treatment, is sufficient to (i) treat or prevent a particular disease, condition, or disorder mediated by PGD2, (ii) attenuate, ameliorate, or eliminate one or more symptoms of the particular disease, condition, or disorder, or (iii) prevent or delay the onset of one or more symptoms of the particular disease, condition, or disorder described herein.

18

[00139| The amount of a compound of Formula 1 that will correspond to such an amount will vary depending upon factors such as the particular compound, disease condition and its severity, the identity (e.g., weight) of the mammal in need of treatment, but can nevertheless be routinely determined by one skilled in the art.

(00140] As used herein, the term "mammal" refers to a warm-blooded animal that has or is at risk of developing a disease described herein and includes, but is not limited to, guinea pigs, dogs, cats, rats, mice, hamsters, and primates, including humans.

(00141) This invention also provides compounds of Formula I for use in the treatment of PGD2- mediatcd conditions.

(00142) An additional aspect of the invention is the use of a compound of Formula I in the preparation of a medicament for therapy, such as for the treatment or prevention PGD2 -mediated conditions.

[00143] The compounds of the present invention can be used in combination with one or more additional drugs, for example an anti-inflammatory compound that works by a different mechanism of action.

[00144] The compounds of the invention may be administered by any convenient route, e.g. into the gastrointestinal tract (e.g. rcclally or orally), the nose, lungs, musculature or vasculature or transdermally. The compounds may be administered in any convenient administrative form, e.g. tablets, powders, capsules, solutions, dispersions, suspensions, syrups, sprays, suppositories, gels, emulsions, patches etc. Such compositions ma)' contain components conventional in pharmaceutical preparations, e.g. diluents, carriers, pH modifiers, sweeteners, bulking agents, and further active agents. If parenteral administration is desired, the compositions will be sterile and in a solution or suspension form suitable for injection or infusion. Such compositions form a further aspect of the invention.

[00145] According to another aspect, the present invention provides a pharmaceutical composition, which comprises a compound of formula (I) or a pharmaceutically acceptable salt thereof, as defined hereinabove. In one embodiment, the pharmaceutical composition includes the compound of formula (I) together with a pharmaceutically acceptable diluent or carrier.

[00146| According to another aspect, the present invention provides a compound of formula (I) or a pharmaceutically acceptable salt thereof, for use in therapy.

[00147] According to a further aspect, the present invention provides the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament to (real an immunologic disorder, as defined hereinabove.

19

[00148] The following examples illustrate the invention. In the examples described below, unless otherwise indicated all temperatures arc set forth in degrees Celsius. Reagents were purchased from commercial suppliers such as Aldrich Chemical Company, Lancaster, TCI or Maybridgc, and were used without further purification unless otherwise indicated. Tctrahydrofuran (THF), dichloromethane (DCM, methylene chloride), toluene, and dioxane were purchased from Aldrich in Sure seal bottles and used as received.

[00149] The reactions set forth below were done generally under a positive pressure of nitrogen or argon or with a drying tube (unless otherwise stated) in anhydrous solvents, and the reaction flasks were typically fitted with rubber septa for the introduction of substrates and reagents via syringe. Glassware was oven dried and/or heat dried.

[00150] ¹ HNMR spectra were obtained as CDCl ₃ , CD ₃ OD, D ₂ O or (I ₆ -DMSO solutions (reported in ppm), using tetramcthylsilane (0.00 ppm) or residual solvent (CDCI ₃ : 7.25 ppm; CD ₃ OD: 3.31 ppm; D ₂ O: 4.79 ppm; d _ό -DMSO: 2.50 ppm) as the reference standard. When peak multiplicities are reported, the following abbreviations arc used: s (singlet), d (doublet), t (triplet), m (multiple.), br (broadened), dd (doublet of doublets), dt (doublet of triplets). Coupling constants, when given, are reported in Hertz (Hz).

Example A

DP-2 binding Inhibition Assay

[00151] The coding sequence of human DP2 was introduced into the human Leukemic cell line K562 by electroporation and stable clones expressing DP2 were obtained by limiting dilution followed by cell surface staining with a rat monoclonal antibody specific for human DP2. Membranes were prepared from one of these DP2 expressing clones and used to determine the ability of the compounds of the present invention to inhibit binding of prostaglandin D2 (PGD2) to its receptor DP2 by the following procedure. Membranes (1.25 Ogλvell) were mixed with ³ H- labeled PGD ₂ and various concentrations of test compounds in 150 μL of binding buffer (50 mM Tris-HCl, pH 7.4, 40 mM MgCl ₃ , 0.1% bovine serum albumin, 0.1% NaN ₃ ) in 96-well U-bottom polypropylene plates. After incubation for 60 minutes at room temperature, the assay was transferred to a filtration plate (#MAFB; Milliporc Corporation, Bedford, MA), and washed three times with binding buffer. Radioactivity was measured by a scintillation counter (TopCount; PerkinElmer Life Sciences, Boston, MA). Nonspecific binding was determined by incubations in the presence of 1 μM unlabeled PGD ₂ or 5 DM of a known DP2 antagonist. IC ₅₀ values for inhibition of binding are determined for each compound tested from the inflexion point of a standard 4-parameter logistical curve fitted to the values obtained. All compounds disclosed herein had IC ₅₀ values less than 1 micromolar.

20

Example 1

2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-((4-fluoropheny lsulfonamido)tnethyl)-

[00152] Step A: 2-(4-hydrox>φhenyl)acetic acid (7.0 g, 46 mmol) was diluted with methane sulfonic acid (100 mL) and cooled to -10 ⁰ C. 2-(Hydroxymcthyl)isoindoline-I,3-dionc (8.2 g, 46 mmol) was added portionwisc over 15 minutes. The reaction was allowed to warm to room temperature and stirred overnight. The reaction was poured onto ice and stirred for 1 hour. The reaction mixture was filtered and rinsed with water. The material was air dried overnight and then placed under high vacuum for 5 hours. The material was then taken up in DMF using heat to ensure all the material was in solution. Small amounts of water were added until a precipitate persisted. The mixture was filtered and the solid material corresponded to bis alkylated material. Additional water was added to crash out the 2-(3-((l,3-dioxoisoindolin-2-yl)methyl)-4-hydroxyphenyl)acet ic acid which was filtered and dried. This material was carried on to the next step without further purification.

[00153] Step B: The product of step A (6.7 g, 22 mmol) was diluted with HBr (21 g, 258 mmol) and acetic acid (20 mL). The reaction mixture was heated to reflux and stirred overnight. The reaction mixture was then cooled to 0 ⁰ C and was neutralized with solid NaOH pellets to pH~10, affording a solution of 2-(3-(aminomethyl)-4-hydroxyphenyI)acetic acid which was used in the next step without further purification.

[00154] Step C: To 2-(3-(aminomethyl)-4-hydroxyphenyl)acctic acid (3.9 g, 21.5 mmol) was added BOC2O (5.40 g, 24.8 mmol) in 30 mL of dioxane. After stirring for 4 hours, the reaction was diluted with ethyl acetate and IN HCl. The organic layer was dried over MgSO ₄ and concentrated. The solid was taken up in minimal methylene chloride, filtered and rinsed with methylene chloride. The filtrate was concentrated to yield 3.0 g of 2-(3-((tert-butoxycarbonyl)aminomethyl)-4- hydroxyphenyl)acctic acid as a white foam.

[00155] Step D: 2-(3-((tert-Butoxycarbonyl)aminomethyl)-4-hydroxyphenyl)acet ic acid (1.6 g, 5.69 mmol) was diluted with THF ( 10 mL) and methanol (5 mL) followed by the addition of TMSCHN ₂ (14.2 mL, 28.4 mmol) dropwise. The reaction mixture was stirred for 30 minutes and then diluted with ethyl acetate and water. The layers were separated and the organic layer was dried

21 over MgSQi, filtered and concentrated. The material was purified using a biotagc 4OS cartridge eluting with hexanes:ethyl acetate (4: 1) yield 860 nig of clear oil that later solidified to methyl 2-(3-

((tcrt-butoxycarbonyl)aminomethyl)-4-hydro.vyphenyl)aceta te.

|00156] Step E: Methyl 2-(3-((tert-butoxycarbonyl)aminomethyl)-4-hydrox\'phenyl)ace tate (200 mg, 0.677 mmol) was diluted with ACN (2 rnL) followed by the addition of K ₂ CO ₃ (206 mg, 1.49 mmol) and l-fluoro-4-nitrobenzene (0.103 mL, 0.948 mmol). The reaction mixture was heated to reflux (at about 82 ⁰ C) and stirred for 5 hours. The reaction mixture was then diluted with ethyl acetate and water. The layers were separated and the organic layer was dried over MgSO.4, filtered and concentrated. The material was purified using a biotage 4OS eluting with hcxancs/cthyl acetate

(3: 1) to yield 242 mg of methyl 2-(3-((tcrt-butoxycarbonyl)aminomcthyl)-4-(4-nitrophcnoxy) phenyl)acctate as a clear oil.

100157] Step F: Methyl 2-(3-((tert-butoxycarbonyl)aminomethyl)-4-(4-niϋOphenoxy) phenyOacetate (242 mg, 0.581 mmol) was diluted with THF (3 mL) followed by the addition of Zn dust (38.0 mg, 0.581 mmol). About 2 mL of saturated ammonium chloride was added dropwise.

After stirring the reaction for 10 minutes, the reaction mixture was diluted with ethyl acetate and saturated sodium bicarbonate. The layers were separated and the organic layer was dried over

MgSO ₄ , filtered and concentrated to yield 225 mg of methyl 2-(4-(4-aminophenoxy)-3-((tert- butoxycarbonyl)aminomelhyl)phenyl)acetate.

|00158] Step G: Methyl 2-(4-(4-aminophenoxy)-3-((tert-butoxycarbonyl)aminomcthyl) phenyOacetate (100 mg, 0.259 mmol) was diluted with methylene chloride (3 mL) followed by the addition of 3,4-dichlorobenzoyl chloride (81.3 mg, 0.388 mmol) and N,N-diisopropylethyl amine

(0.0451 mL, 0.259 mmol). The reaction was stirred for 3 hours. The reaction mixture was diluted with methylene chloride and water. The layers were separated and the organic layer was dried over

MgSO ₄ , filtered and concentrated. The material was purified using a biotagc 12i eluting with hexanes:cthyl acetate (3: 1) to yield 130 mg of methyl 2-(3-((tert-butoxycarbonyl)aminomethyl)-4-

(4-(3,4-dichlorobenzamido)phcnoxy)phenyl)acctatc.

]00159] Step H: Methyl 2-(3-((tert-butoxycarbonyl)aminomethyl)-4-(4-(3,4- dichlorobenzamido)phenoxy)phenyl)acetatc (130 mg, 0.232 mmol) was treated with HCl (0.581 mL,

2.32 mmol), After stirring for 3 hours, the material was concentrated to yield 100 mg of methyl 2-

(3-(aminomethyl)-4-(4-(3,4-dichlorobenzamido)phenoxy)phen yl)acetate.

[00160] Step I: The product of step H (30 mg, 0.065 mmol) was diluted with methylene chloride

(1 mL) followed by the addition of 4-fluorobenzene-l-sulfonyl chloride (15 mg, 0,078 mmol) and

DlEA (0.024 mL, 0.14 mmol). After stirring the reaction for 4 hours, the reaction mixture was placed directly onto a preparative tic plate 0.5 mm and eluted with hexanesxthyl acetate (3: 1) to

22 yield 30 mg of methyl 2-(4-(4-(3,4-dichlorobenzamido)phenoxy)-3-((4-fluorophenyl- sulfonamido)methyl)phenyl)acetate.

|00161] Step J: Methyl 2-(4-(4-(3,4-dichlorobenzamido)phenoxy)-3-((4-fiuorophenyl- sulfonamido)mcthyl)phenyl)acetate (40 mg, 0.065 mmol) was diluted with MeOH (1 mL) followed by the addition of NaOH (0.32 mL, 1.3 mmol). After stirring for I hour, the reaction mixture was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated. The material was purified using a 0.5 mm preparative plate cliiUng with methylene chloridc:MeOH:AcOH (90:9: 1) to yield 12 mg of the title compound as a solid. ¹ H NMR (400 MHz, CDCI _3/ CD ₃ OD) 8.1 (m, IH), 7.85 (m, I H), 7.8 (m, 2H), 7.7 (d, IH), 7.6 (d, IH), 7.25 (s, 1 H), 7.2 (m, 3H), 6.85 (d, 2H), 6.7 (d, IH), 4.15 (s, 2H), 3.55 (s, 2H).

Example 2 2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-(methylsulfonamido methyl)phenyl)acetic acid

[00162] Prepared by the method of Example 1, substituting 4-fluorobenzene- l-sulfonyl chloride in Step H with methanesulfonyl chloride. ¹ H NMR (400 MHz, ^-DMSO) 12.4 (s, IH), 10.4 (s, IH), 8.20 (m, 1 H) _: 7.95 (m, IH), 7.85 (d, IH), 7.75 (d, 2H), 7.55 (t, IH), 7.4 (m, I H), 7.19 (m, IH), 7.05 (d, 2H), 6.8 (d, IH), 4.2 (d, 2H), 3.55 (s, 2H), 2.85 (s, 3H).

Example 3

2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-((2,4-dichlorop henylsulfonamido)methyl)- phenyl)acetic acid

(00163] Prepared by the method of Example 1 , substituting 4-fluorobenzene- l-sulfonyl chloride in Step H with 2..4-dichiorobcnzyl-l-sulfonyl chloride. ¹ H NMR (400 MHz, CDCl ₃ ,CDjOD) 8.1 (s, IH), 7.85 (m, 2H), 7.65 (m, 3H), 7.50 (m, IH), 7.35 (d, IH), 7.2 (s, IH), 7. 10 (d, I H), 6.90 (d, 2H), 6.60 (d, IH), 4.20 (s, 2H), 3.45 (s, 2H).

23

Example 4 2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-(nicotinamidomethy l)phenyl)acetic acid

(00164] Step A: 4-Aminophenol (1.9 g, 17 mmol) was diluted with DMF (40 mL) followed by the addition of pyridine (0.978) (1.4 mL, 17 mmol) and 3,4-dichlorobenzoyl chloride (3.0 g, 14 mmol) in 5 mL of DMF. After stirring for 12 hours, the reaction mixture was diluted with ethyl acetate and 2N HCl. The aqueous layer was extracted once with ethyl acetate. The organics were combined, washed with water, brine, dried over MgSO ₄ , filtered and concentrated onto silica gel. The material was purified using a biotage 4OM column eluting with hexanes:cthyl acetate (9: 1) using 2 L and then (60:40) IL to yield 3,4-dichloro-N-(4-hydroxyphenyl)-benzamide ( 1.5 g, 37% yield) as a white solid.

[00165| Step B: tert-Butyl 2-(3-cyano-4-fluorophenyl)acetate (2.0 g, 8.5 mmol) and the product of step A (2.9 g, 10 mmol) were diluted with DMSO (22 mL) followed by the addition of potassium carbonate (1.4 g, 10 mmol). The reaction was heated to 125 ⁰ C and stirred for 12 hours. The reaction mixture was then cooled to room temperature, diluted with ethyl acetate and 10% aqueous sodium carbonate. The layers were separated and the organic layer was washed with 10% aqueous sodium carbonate two more times followed by water and brine. The organic material was dried over MgSO4, filtered and concentrated. The material was purified using a biotage 4OM column eluting with hexancsicthyl acetate (8:2) to yield tert-butyl 2-(3-cyano-4-(4-(3,4- dichlorobcnzamido)phenoxy)phenyl)acetatc (2.5 g, 59% yield) as a white solid. (00166| Step C: The product of step B (100 mg, 0.201 mmol) was diluted with 7N ammonia/methanol (10 mL), followed by addition of ethyl acetate (10 mL). Once the material dissolved, Raney Nickel (1.72 mg, 0.0201 mmol) was added and die reaction was purged three times with a balloon of hydrogen. After stirring for 12 hours, the reaction mixture was filtered through a GF/F filter and concentrated. The residue was purified using a biotage 12i column eluting with methylene chloride:MeOH:NH ₄ OH (90:9: 1) to yield tert-butyl 2-(3-(aminomethyl)-4-(4-(3,4- dichlorobcnzamido)phenoxy)phcnyl)acetate (100 mg, 99.2% yield) as a clear oil.

24

[00167] Step D: The product of step C (0.050 g, 0.997 mmol) was dissolved into I mL of DCM in a small test tube. Pyridine (0. 017 g, 0.219 mmol) and nicotinoyl chloride (0.0195 g, 0.110 mmol) were added, and the reaction mixture was stirred for 12 hours. The reaction mixture was then diluted with methylene chloride and water. The methylene chloride layer was separated and loaded directly onto a biotagc 12i column cluting with methylene chloridc:MeOH (95:5) to yield tert-buryl 2-(4-(4-(3,4-dichlorobenzamido)phcnoxy)-3-(nicotinamidomethy l)phenyl)-acetate (44 mg, 73% yield) as a clear oil.

[00168] Step E: The product of step D (44 mg _; 0.073 mmol) was diluted with methylene chloride:TFA (1 : 1 ) and stirred for 3 hours. The reaction mixture was concentrated, taken up in ether and sonicated. The solid was filtered, rinsed with ether and dried under vacuum to yield the title compound (35 mg, 88% yield) as white solid. ¹ H NMR (400 MHz, CDCI ₃ /CD ₃ OD) 8.75 (m, IH), 8.65 (m, IH), 8.15 (m, IH), 8. 10 (m, IH), 8.0 (m, IH), 7.60 (m, 3H), 7.45 (m, IH), 7.20 (m, IH), 6.95 (d, 2H), 6.90 (d, IH), 4.65 (m, 2H), 3.62 (s, 2H).

Example 5

2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-((N,N-dimethyls ulfamoylamino)methyl)-

[00169] Prepared by the method of Example 4, substituting nicotinoyl chloride in Step D with dimcthylsulfamoyl chloride. ¹ H NMR (400 MHz, CDC1 _3/ CD ₃ OD) 8.05 (s, IH), 7.80 (m, IH), 7.65 (m,2H), 7.55 (d, IH), 7.35 (m, IH), 7.18 (m, IH), 7.00 (d, 2H), 6.82 (d, IH), 4.25 (s, 2H), 3.60 (s, 2H), 2.75 (s, 6H).

25

Example 6 2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-(methylsulfonamido methyl)phenyl)acetic acid

[00170] Prepared by the method of Example 4, substituting nicotinoyl chloride in Step D with methanesulfonyl chloride. ¹ H NMR (400 MHz, αk-DMSO) 12.4 (s, IH), 10.4 (s, I H), 8.20 (m, IH), 7.95 (m, IH), 7.85 (d, IH), 7.75 (d, 2H), 7.55 (t, I H), 7.4 (m, IH), 7.19 (m, IH), 7.05 (d, 2H), 6.8 (d, IH), 4.2 (d, 2H), 3.55 (s, 2H), 2.85 (s, 3H).

Example 7

2-(3-(Cyclohexanecarboxamidomethyl)-4-(4-(3,4-dichloroben zamido)phenoxy)phenyl)-acetic acid

(001711 Prepared by method of Example 4, substituting nicotinoyl chloride in Step D with cyclohexanccarbonyl chloride. ¹ H NMR (400 MHz, CDC1 ₃ ,CD ₃ OD) 8.10 (m, IH), 7.80 (m, IH), 7.65 (d, 2H), 7.55 (d, IH), 7.25 (m, I H), 7. 15 (d, I H), 6.95 (d, 2H), 6.85 (d, IH), 2.10 (m, IH), 1.6- 1.8 (m, 5H), 1.2-1.4 (m, 6H).

Example 8 2-(3-(Acetamidomethyl)-4-(4-(3,4-dichlorobenzamido)phenoxy)p henyl)acetic acid

26

|00172) Prepared by the method of Example 4, substituting nicotinoyl chloride in Step D with acetyl chloride. ¹ H NMR (400 MHz, CDCI ₃ /CD ₃ OD) 8.10 (m, IH), 7.80 (m, IH), 7.60 (m, 3H), 7.30 (m, IH), 7.15 (m. IH), 6.95 (d, 2H), 6.85 (d, I H), 4.42 (s, 2H), 3.40 (s, 2H), 1.95 (s, 3H).

Example 9 2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-(isonicotinamidome thyl)phenyl)acetic acid

[00173] Prepared by the method of Example 4, substituting nicotinoyl chloride in Step D with isonicotinoyl chloride. ¹ H NMR (400 MHz, CDCI ₃ /CD ₃ OD) 8.62 (m, IH), 8.05 (m, IH), 7.88 (m, IH), 7.80 (m, I H), 7.65 (m, 2H), 7.58 (m, 3H), 7.35 (m, IH), 7.20 (m, I H), 6.95 (d ₅ 2H), 6.90 (d, IH), 4.65 (d, 2H), 3.62 (s, 2H).

27

Example 10 2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-((4-f1uorobenzamid o)methyl)phenyl)acetic acid

|00I74) Prepared by the method of Example 4, substituting nicotinoyl chloride in Step D with 4- fiuorobenzoyl chloride. ¹ H NMR (400 MHz, CD ₃ OD) 8.10 (s, IH), 7.85 (m, IH), 7.80 (m, 2H), 7.70 (d, I H), 7.62 (d, 2H), 7.35 (s, IH), 7.25 (d, IH), 7.35 (m, 2H), 6.95 (d, 2H), 6.90 (d, IH), 4.62 (s, 2H), 3.60 (s, 3H).

Example 11 2-(4-(4-(3,4-Dichlorob hyl)phenyl)acetic acid

[0017Sj Prepared by the method of Example 4, substituting nicotinoyl chloride in Step D with picolinoyl chloride. ¹ H NMR (400 MHz, CD ₃ OD) 8.60 (d, IH), 8.10 (m, I H), 8.05 (m, IH), 7.95 (m, IH), 7.85 (m, I H), 7.70 (d, IH), 7.60 (d, 2H), 7.55 (m, IH), 7.35 (m, I H), 7.20 (d, IH), 7.00 (d, 2H), 6.85 (d, IH), 4.64 (s, 2H), 3.60 (s, 2H).

Example 12

2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-((methoxycarbon ylamino)methyl)phenyl)-acetic acid

28

[00176| Prepared by the method of Example 4, substituting nicotinoyl chloride in Step D with methyl chloroformate. ¹ H NMR (400 MHz, CDCl ₃ ) 8.10 (m, IH), 7.80 (m, IH), 7.65 (m, 2H), 7.58 (m, IH) ₁ 7.30 (m, IH), 7.19 (m, IH), 6.95 (m, 2H), 6.85 (m, IH), 4.40 (s, 2H), 3.65 (s ₅ 3H), 3.60 (s, 2H).

Example 13

2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-((pyridine-3-su lfonamido)methyl)phenyl)-acetic acid

[00177] Prepared by the method of Example 4. substituting nicotinoyl chloride in Step D with pyridine-3-sulfonyl chloride. ¹ H NMR (400 MHz, CDCl ₃ ) 8.95 (s, IH), 8.60 (m, IH), 8.10 (m, IH), 8.05 (d, IH) ₅ 7.80 (d, IH), 7.60 (m, 3H), 7.35 (m, I H), 7.22 (m, I H), 7.05 (d, I H), 6.80 (d, 2H), 6.62 (d, IH), 4.25 (s, I H), 3.55 (s, 2H).

Example 14

2-(4-(4-(3,4-Dichlorophenylcarbamoyl)phenoxy)-3-(methylsu lfonamidomethyl)phenyl)-acetic acid

[00178] Step A: tert-Butyl 2-(3-cyano-4-fluorophenyl)acetatc (2.6 g, 11.1 mmol) and methyl 4- hydroxybenzoate (3.36 g, 22.1 mmol) were diluted with DMSO (22 mL) followed by the addition of potassium carbonate (1.83 g, 13.3 mmol). The reaction mixture was heated to 125 ⁰ C and stirred for 5 hours. The reaction mixture was diluted with ethyl acetate and 10% aqueous sodium carbonate. The layers were separated and the organic layer was washed with 10% aqueous sodium carbonate two more times followed by water and brine. The organic material was dried over MgSO ₄ , filtered and concentrated. The material was purified using a biotage 4OM column eluting with hexanes:ethyl

29 acetate (9: 1) to yield methyl 4-(4-(2-tert-butoxy-2-oxoethyl)-2-cyanophenoxy)benzoate (2.65 g, 65.3% yield) as a clear oil.

|00179] Step B: The product of step A (2.5 g, 6.80 mmol) was diluted with TN ammonia/niethanol (30 mL) followed by the addition of Raney Nickel (0.0583 g, 0.680 mmol). The reaction mixture was purged three times with hydrogen and stirred for 12 hours. The reaction mixture was then filtered through a GF/F filter and concentrated. The residue was purified using a biotage 4OM column cluting with methylene chloride:MeOH:NH,OH (90:9: 1) to yield methyl 4-(2- (aminomcmyl)-4-(2-tert-butθλy-2-oxocthyl)phenoxy)benzoatc (1.87 g, 74.0% yield) as a clear oil. [00180] Step C: The product of step B ( 1.87 g, 5.03 mmol) was diluted with methylene chloride (2 mL) followed by the addition of pyridine (0.489 mL, 6.04 mmol) and methanesulfonyl chloride (0.779 mL, 10.1 mmol). After stirring for 12 hours, the reaction mixture was diluted with methylene chloride and 2N HCl, the layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated. The material was purified using a biotage 4OM cartridge eluting with hcxancsxthyl acetate (1: 1) to yield methyl 4-(4-(2-tcrt-buloxy-2-oxoethyl)-2- (methylsulfonamidomethyl)phcnoxy)bcnzoate (1.53 g, 67.6% yield) as a clear oil. ]0018l] Step D: The product of step C (900 mg, 2.00 mmol) was diluted with dioxane (10 mL) followed by the addition of LiOH-H ₂ O (126 mg, 3.00 mmol) dissolved in water (2 mL). After stirring for 12 hours, the reaction mixture was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSU4, filtered and concentrated. The material was purified using a biotage 4OM cartridge cluting with methylene chloridc:MeOH (95:5) to yield 4- (4-(2-tert-butox)'-2-oxoethyl)-2-(mcthylsulfonamido-melhyl)p henoxy)bcnzoic acid (710 mg, 81.4% yield) as a clear oil.

[00182] Step E: The product of step D (62 mg, 0.14 mmol) was diluted with methylene chloride (1 mL) followed by the addition of oxalyl dichloride (0.093 mL, 0.19 mmol) and 1 drop of DMF. The reaction was stirred for 30 minutes followed by the addition of 3,4-dichlorobenzenamine (46 mg, 0.28 mmol). The reaction was allowed to stir for 1 hour. The reaction mixture was loaded directly onto a 12i sim and purified on the horizon eluting with methylene chloride:MeOH (99.5-0.5 to 95:5) to yield 30 mg of tert-butyl 2-(4-(4-((3,4-dichlorophenyl)carbamoyl)phenoxy)-3- (metliylsulfonamidomethyl)phenyl)acetate as a clear oil.

[00183] Step F: The product of step E (22 mg, 0.038 mmol) was diluted with methylene chloride (1 mL) followed by the addition of TFA (1 mL). After stirring for 1 hour, the reaction was concentrated, diluted with ether, sonicated and concentrated to yield 2-(4-(4-((3,4- dichlorophenyl)carbamoyl)phenoxy)-3-(metliylsulfonamidomethy l)phenyl)acetic acid (15 mg, 75%

30 yield) as a white solid. ¹ H NMR (400 MHz, CDCyCD ₃ OD) 7.92 (m, I H), 7.90 (d, 2H), 7.59 (m, IH), 7.42 (m, 2H), 7.25 (m, IH), 7.10 (d, 2H), 6.90 (d, IH), 4.28 (s, 2H), 3.62 (s, 2H), 2.85 (s, 3H).

Example 15

2-(4-(4-(3,4-DichlorophenethylcarbamoyI)phenoxy)-3-(methy lsulfonainidomethyl)- phenyl)acetic acid

[00184] Prepared by the method of Example 14, substituting 3,4-dichlorobenzenamine in Step E with 2-(3,4-dichlorophenyl)cthanamine. ¹ H NMR (400 MHz, CDCIvCD ₁ OD) 7.75 (d, 2H), 7.35 (m, 3H), 7.22 (d, IH), 7.10 (d, IH), 6.95 (d, 2H), 6.90 (d, IH), 4.25 (s, 2H), 3.62 (m, 4H), 3.40 (s, 2H), 2.90 (t, 2H), 2.85 (s, 3H).

Example 16 2-(3-(MethyIsulfonamidomethyl)-4-(4-(naphthalen-2-ylcarbamoy l)phenoxy)phenyl)-acetic acid

[00185] Prepared by the method of Example 14, substituting 3,4-dichlorobenzenamine in Step E with napthaien-2-amine. ¹ H NMR (400 MHz, CDC1 ₃ /CD ₃ OD) 8.35 (s, I H), 7.96 (d, 2H), 7.83 (m, 3H), 7.70 (d, IH), 7.45 (m, 3H), 7.25 (d, I H), 7.05 (d, 2H), 6.95 (d, I H), 4.30 (d, 2H), 3.62 (s, 2H), 2.85 (s, 3H).

31 Example 17

2-(4-(4-(4-Fluorophenethylcarbamoyl)phenoxy)-3-(methylsul fonamidomethyl)phenyl)-acetic acid

[00186] Prepared by the method of Example 14, substituting 3,4-dichlorobenzcnamine in Step E with 2-(4-fluorophenyl)ethanamine. ¹ H NMR (400 MHz, CDCI3/CD3OD) 7.70 (d, 2H), 7.40 (m, IH), 7.22 (m, 3H), 7.10 (m, 2H), 6.90 (d, 2H), 6.70 (d, IH), 4.30 (s, 2H), 3.65 (m, 4H), 2.90 (t, 2H), 2.85 (s, 3H).

Example 18

2-(3-(Methylsulfonamidoniethyl)-4-(4-(phenethylcarbamoyl) phenoxy)phenyl)acetic acid

[00187] Prepared by the method of Example 14, substituting 3,4-dichlorobcnzcnamine in Step E with phencthylainine. ¹ H NMR (400 MHz, CDCyCD ₃ OD) 7.70 (d, 2H), 7.40 (m, IH), 7.31 (m, IH), 7.25 (m, 5H), 6.95 (d, 2H), 6.90 (d, IH), 4.30 (s, 2H), 3.68 (t, 2H), 3.61 (s, 2H), 2.90 (t, 2H), 2.85 (s, 3H).

Example 19 2-(3-(Methylsulfonamidomethyl)-4-(4-(phenylcarbamoyl)phenoxy )phenyl)acetic acid

32

[00188] Prepared by the method of Example 14, substituting 3,4-dichlorobenzenamine in Step E with aniline. ¹ H NMR (400 MHz, CDCI ₃ /CD ₃ OD) 7.92 (d, 2H) ₅ 7.65 (d, 2H), 7.45 (s, IH), 7.38 (m, 2H), 7.25 (d, IH), 7.18 (m, I H), 7.05 (d, 2H), 6.95 (d, IH), 4.30 (s, 2H), 3.65 (s, 2H), 2.85 (s, 3H).

Example 20 2-(4-(4-(Benzylcarbamoyl)phenoxy)-3-(methylsulfonamidomethyl )phenyl)acetic acid

|00189] Prepared by the method of Example 14, substituting 3,4-dichlorobcnzcnamine in Step E with benzyl amine. ¹ H NMR (400 MHz, CDCl ₃ ZCD ₃ OD) 7.80 (d, 2H), 7.40 (m, IH), 7.35 (m, 4H), 7.22 (d, IH), 6.95 (m, 3H), 6.90 (d, IH), 4.62 (d, 2H), 4.30 (s, 2H), 3.62 (s, 2H), 2.85 (s, 3H).

Example 21

2-(4-(4-(4-Chloι ophenethylcarbamoyl)phenoxy)-3-(methylsulfonamidomethyl)phen yl)-acetic acid

(00190] Prepared by the method of Example 14, substituting 3,4-diclilorobcnzcnamine in Step E with 2-(4-chlorophenyI)cthanamine. ¹ H NMR (400 MHz, CDCI ₃ /CD ₃ OD) 7.7 (d. 2H), 7.4 (s, IH), 7.25 (d, 2H), 7.22 (d, I H), 7.18 (d, 2H), 6.95 (d, 2H), 6.90 (d, IH), 6.82 (br t, IH), 4.3 (s, 2H), 3.65 (t, 2H), 3.4 (br s, 2H), 2.9 (t, 2H), 2.85 (s, 3H).

Example 22

2-(4-(4-(2-(4-Chlorobenzylamino)-2-oxoethyl)phenoxy)-3-(m ethylsulfonamidomethyl)-

33

J00191| Prepared by the method of Example 14, substituting methyl 4-hydroxybenzoate in Step A with methyl 2-(4-hydroxylphenyl)acetate and substituting 3,4-dichlorobenzenaminc in Step E with 4-chlorobenzyl amine. ¹ H NMR (400 MHz, CDCVCD ₃ OD) 7.38 (m, IH), 7.25 (m, 4H), 7.20 (m, 3H), 6.95 (d, 2H) _; 6.85 (d, IH), 4.38 (m, 2H), 4.32 (s, 2H), 3.61 (s, 2H) ₁ 3.55 (s, 2H), 2.85 (s, 3H).

Example 23

2-(4-(4-(2-(Benzylamino)-2-oxoethyl)phenoxy)-3-(methylsul fonamidomethyl)phenyl)-acetic acid

|00192] Prepared by the method of Example 14, substituting methyl 4-hydroxybcnzoate in Step A with methyl 2-(4-hydroxylphenyl)acetate and substituting 3,4-dichlorobenzcnaminc in Step E with benzylaminc. ¹ H NMR (400 MHz, CDCI ₃ /CD ₃ OD) 7.38 (m, IH), 7.15-7.30 (m, 8H), 6.95 (d, 2H), 6.85 (d, IH), 4.40 (d, 2H), 4.32 (s, 2H), 3.61 (s, 2H), 3.56 (s, 2H), 2.82 (s, 3H).

Example 24

2-(3-(Methylsulfonamidoniethyl)-4-(4-(2-oxo-2-(phenethyla mino)ethyl)phenoxy)phenyl)-acetic acid

[00193| Prepared by the method of Example 14, substituting methyl 4-hydroxybenzoate in Step A with methyl 2-(4-hydroxylphenyl)acetate and substituting 3,4-dichlorobenzenaminc in Step E with phenethylamine. ¹ H NMR (400 MHz, CDCI ₃ /CD ₃ OD) 7.38 (m, IH), 7.28 (m, I H), 7.25 (m, IH), 7.18-7.22 (111, 2H), 7.15 (d, 2H), 7.10 (d, IH), 6.92 (d, 2H), 6.82 (d, IH), 4.38 (s, 2H), 3.62 (s, 2H), 3.48 (m, 4H), 2.82 (s, 3H), 2.75 (t, 2H).

34

Example 25

2-(4-(4-(2-(Benzyl(methyl yIsulfonaitiidomethyl)-

[00194 j Prepared by the method of Example 14, substituting methyl 4-hydroxybenzoatc in Step A with methyl 2-(4-hydroxylphenyl)acelate and substituting 3,4-dichlorobcnzenamine in Step E with N-methylbcnzj'lamine. ¹ H NMR (400 MH7, CDCI ₃ /CD ₃ OD) 7. 10-7.40 (m, 9H), 6.94 (m, 2H), 6.82 (m, I H), 4.60 (d, 2H), 4.35 (m, 2H), 3.75 (d, 2H), 3.62 (m, 2H), 2.97 (d, 3H), 2.82 (s, 3H).

Example 26

2-(3-(Methylsulfonamidomethyl)-4-(4-(2-oxo-2-(phenylamino )ethyl)phenoxy)phenyl)-acetic acid

|00195) Prepared by the method of Example 14, substituting methyl 4-hydroxybcnzoate in Step A with methyl 2-(4-hydroxylphenyl)acetate and substituting 3,4-dichlorobcnzenamine in Step E with aniline. ¹ H NMR (400 MHz, CDCI ₃ /CD ₃ OD) 7.55 (d, 2H), 7.30-7.35 (m, 5H), 7.18 (m, IH), 7.10 (m, I H), 6.95 (d, 2H), 6.85 (d, IH), 4.35 (s, 2H), 3.68 (s, 2H), 3.60 (s, 2H), 2.85 (s, 3H).

Example 27 2-(4-(4-Benzamidophenoxy)-3-((4-fluoι ophenylsulfonamido)methyl)phenyl)acetic acid

[00196] Prepared by the method of Example 1 substituting 3,4-dichlorobenzoyl chloride in Step G with benzoyl chloride. MS+ 534.9 |M + IJ.

35 Example 28

2-(4-(4-(4-Chlorobenzamido)phenoxy)-3-((4-fluorophenylsul fonamido)methyl)phenyl)-acetic acid

f00197J Prepared by the method of Example 1 substituting 3,4-dichlorobcnzoyl chloride in Step G with 4-chlorobenzoyl chloride. ¹ H NMR (400 MHz, CD ₃ OD) 7.85 (d, 2H), 7.70 (m, IH), 7.45- 7.55 (m, 5H), 7.35 (m, IH), 7.15 (m, 2H), 6.60-6.70 (m, 3H), 4.55 (s, 2H), 3.58 (s, 2H).

Example 29

2-(4-(4-(3-Chlorobenzamido)phenoxy)-3-((4-fluorophenylsul fonamido)methyl)phenyl)-aceϋc

J00198) Prepared by the method of Example 1 substituting 3,4-dichlorobenzoyl chloride in Step G with 3-chlorobenzoyl chloride ¹ H NMR (400 MHz, CD ₃ OD) 7.90 (s, IH), 7.80 (d, IH), 7.65 (m, IH), 7.52 (m, 4H), 7.45 (t, IH), 7.35 (m, IH), 7.15 (m, 2H), 6.60 (m, 4H), 4.45 (s, 2H), 3.50 (br s, 2H).

Example 30

2-(4-(4-(3,4-Difluorobenzamido)phenoxy)-3-(methylsulfonam idomethyl)phenyl)acetic acid

36

[00199| Prepared by the method of Example 1 substituting 3,4-dichlorobenzoyl chloride in Step G with 3,4-difluorobenzoyl chloride and substituting 4-fluorobenzenc- 1-sulfonyl chloride in Step I with mcthanesulfonyl chloride. ¹ H NMR (400 MHz, CD ₃ OD) 7.88 (m IH), 7.80 (m, IH), 7.65 (d, 2H), 7.42 (m, 2H), 7.20 (m, 2H), 7.00 (d, 2H), 6.82 (m, I H), 4.33 (s, 2H), 3.62 (s, 2H), 2.85 (s, 3H).

Example 31 2-(4-(4-(2-Chlorobenzamido)phenoxy)-3-(methylsuIfonamidoniet hyl)phenyl)acetic acid

[00200| Prepared by the method of Example 1 substituting 3,4-dichlorobenzoyl chloride in Step G with 2-chlorobenzoyl chloride and substituting 4-fluorobcnzcnc- 1-sulfonyl chloride in Step I with methancsulfonyl chloride. ¹ H NMR (400 MHz, CD ₃ OD) 7.68 (d, 2H), 7.40-7.55 (m, 5H), 7.20 (d, IH), 7.05 (d, 2H), 6.85 (d, I H), 4.35 (s, 2H), 3.65 (br s, 2H), 2.85 (s, 3H).

Example 32 2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-methoxyphenyl)acet ic acid.

[00201] Prepared according to Steps E, F, G and J of Example, 1, substituting methyl 2-(3-((tert- butoxycarbonyl)aminomethyl)-4-hydroxyphenyl)acetate in Step E with methyl 2-(4-hydroxy-3- methoxyphenyl)acetate. ¹ H NMR (400 MHz, CDCl ₃ ) 9.64 (s, IH), 8.17 (d, IH), 7.97 (dd, IH), 7.72- 7.75 (m, 4H), 7.13 (d, IH), 6.99 (d, IH), 6.93 (d, IH), 6.87 (m, 2H), 3.80 (s, 3H), 3.66 (s, 2H).

37

Example 33 2-(4-(4-(benzyloxycarbamoyl)phenoxy)-3-(methylsulfonamidomet hyI)phenyl)acetic acid

|00202] Prepared by the method of Example 14, substituting 3,4-dichlorobenzenamine in Step E with O-benzylhydroxylamine hydrochloride. ¹ H NMR (400 MHz, CD ₃ OD) 7.76 (d, J=8.6 Hz, 2H), 7.50 (t, J = 6.3 Hz, IH), 7.33-7.48 (m, 5H), 7.23 (d, J=6.3 Hz, IH), 6.99 (d, J=8.7 Hz, 2H), 6.91 (d, J= 8.4 Hz, 2H), 4.91 (s, 2H), 4. 10 (d, J=6.3 Hz, 2H), 3.60 (s, 2H), 2.85 (s, 3H).

Example 34 2-(3-Chloι o-4-(4-(4-chlorophenethylcarbamoyl)phenoxy)phenyl)acetic acid

(00203| Step A: 2-(3-Chloro-4-hydroxyphenyI)acetic acid (5.0 g, 26.8 mmol) was dissolved in 30 mL of MeOH and H ₂ SO ₄ (0.744 ml, 13.4 mmol) was added dropwise at ambient temperature. The reaction was refluxed for four hours and then concentrated in vacuo. The residue was diluted with 150 mL of EtOAc and washed sequentially with saturated NaHCOj, water, and brine, dried over H2SO4, and concentrated in vacuo to provide methyl 2-(3-chloro-4-hydroxyphcnyl)acetate (5.16 g, 96.0% yield) as a semi-opaque thick oil.

(00204] Step B: Methyl 2-(3-chloro-4-hydroxyphcnyl)acetatc (177 mg, 0.885 mmol) was diluted with DMSO (3 mL) followed by the addition of K ₂ CO ₃ ( 122 mg, 0.885 mmol) and N-(4- chlorophcncthyl)-4-chloro-3-nitrobcnzamide (300 mg, 0.885 mmol) (synthesized in step C). The reaction was heated to 85 ⁰ C and stirred for 12 hours. The reaction was cooled, diluted with DCM and washed with 10% aq. sodium carbonate, water and brine. The layers were separated and the organic layer was dried over H ₂ SO4, filtered and concentrated. The material was purified using a biotage 4OS cartridge eluϋng with DCM:MeOH (99: 1) to yield methyl 2-(4-(4-((4- chlorophcncthyl)carbamoyl)-2-nitrophenoxy)-3-chlorophcnyl)ac ctate (206 mg, 46.3% yield).

38

[00205| Step C: 2-(4-Chlorophenyl)ethanamine (2.3 ml, 16 mmol) was diluted with DCM (40 niL) followed by the addition of DlEA (2.9 ml, 16 mmol) and 4-chloro-3-nitrobenzoyl chloride (3.0 g, 14 mmol) in 10 mL of DCM. After stirring for 30 minutes the reaction was loaded directly onto a biotage 4OM cartridge and elutcd with hexanes:ethyl acetate (2: 1) to yield N-(4-chlorophenethyI)-4- chloro-3-nitrobenzamide (4.0 g, 86% yield) as a white solid.

[00206] Step D: Methyl 2-(4-(4-((4-chlorophenethyl)carbamwl)-2-nilrophenoxy)-3- chlorophenyOacetatc (1 10 mg, 0.219 mmol) was diluted with THF ( I mL) followed by the addition of Zn dust (14.3 mg, 0.219 mmol) and saturated NH ₄ CI (1 mL). After stirring for 1 hour, the reaction was diluted DCM and 10% aq. sodium carbonate. The layers were separated and the organic layer was dried over MgSC>4, filtered and concentrated to yield methyl 2-(4-(4-((4- chlorophenethyl)carbamoyl)-2-aminophenoxy)-3-chlorophenyl)ac etate (102 mg, 98.6% yield). (00207) Step E: Isobutyl nitrite (0.0751 ml, 0.634 mmol) was diluted with DMF (1 mL), placed under nitrogen and heated to 60 ⁰ C. Methyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)-2- aminophenoxy)-3-chlorophenyl)acetatc (100 mg, 0.21 1 mmol) in DMF (1 mL) was added dropwise and the reaction was stirred for 1 hour. The reaction was cooled, diluted with ethyl acetate and washed with 2N HCl, saturated sodium bicarbonate and brine. The organic layer was dried over MgSO ₄ , filtered and concentrated. The material was purified using a biotage 12i column, cluting with DCM: MeOH (99: 1) to yield methyl 2-(4-(4-((4-chlorophcncthyl)carbamoyl)phcnoxy)-3- chlorophenyOacctate (70 mg, 72.3% yield).

[00208] Step F: Methyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3-chloropheny l)acetate (33 mg, 0.0720 mmol) was diluted with THF (500 μL) followed by the addition NaOH (0.144 ml, 0.720 mmol) and 100 μL of water. After stirring for 2 hours, the reaction was diluted with DCM and 2N HCI. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated to yield 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3-chloropheny l)acetic acid (19 mg, 59.4% yield) as a white solid. ¹ H NMR (400 MHz, CDC1 ₃ /CD ₃ OD) δ 7.65 (d, 2H), 7.42 (m, IH), 7.25 (d, 2H), 7.20 (d, IH), 7.15 (d, 2H), 7.05 (d, IH), 6.85 (d, 2H), 3.65 (t, 2H), 3.60 (s, 2H), 2.90 (t, 2H).

Example 35 2-(3-cyano-4-(4-(3,4-dichlorobenzamido)phenoxy)phenyl)propan oic acid

39

[00209| Step A: To a stirred solution of diisopropylaminc (357 μl, 2.55mmol) in THF (3 ml) at - 78 ⁰ C was added n-butyllithium (2.5M in hcxancs, 1.02 ml, 2.55 mmol). The reaction was stirred for 30 minutes after which time a solution of the acetate (500 mg, 2.13 mmol) in THF (3.5 ml) was added dropwise and stirred for 20 minutes at -78 ⁰ C, then warmed to 0 ⁰ C. MeI (133 μl, 2.13 mmol) was added and the reaction stirred for an additional 30 minutes. The reaction was acidified with 2M HCl and extracted with EtOAc. The combined organics were then washed with brine and dried over MgSO ₄ . The residue was purified on the Biotage Horizon (40+M, 5%to 50% BiEtOAc, 21 ml-1008 ml) to give tcrt-butyl 2-(3-cyano-4-fluorophcnyl)propanoatc (0.368 g, 1.48 mmol, 70%). ¹ H NMR (400 MHz, CDCl ₃ ) δ 7.50-7.60 (m, 2H), 7.17 (t, J=8.7 Hz, I H), 3.63 (q, J=7.1 Hz, IH), 4.60 (d, J=7.2 Hz, 3H), 1.40 (s, 9H).

[00210] Step B: To a stirred solution of 4-aminophenol (2.50 g, 22.9 mmol) in DMF (55 ml) was added pyridine (1.85 ml, 22.9 mmol) followed by 3,4-dichlorobenzoyl chloride (4.0 g, 19.1 mmol) in DMF (7 ml). The reaction stirred at ambient temperature overnight, then diluted with 2M HCl and EtOAc. The aqueous layer was then extracted with EtOAc and the combined organics washed with brine and dried over MgSO ₄ . The residue was purified on the Biotage Horizon (65+M 5% to 75% B:EtOAc, 51 ml-2448 ml). 3,4-Dichloro-N-(4-hydroxyphenyl)bcnzamide was collected as a white solid (3.91 g, 13.9 mmol, 73%). ¹ H NMR (400 MHz, CD ₃ OD) δ 8.09 (s, 1 H) _S 7.84 (d, J=8.5 Hz, IH), 7.67 (d, J=8.6 Hz, IH), 7.45 (d, J=8.8 Hz, 2H), 6.78 (d, J=8.4 Hz, 2H).

[00211 | Step C: To a stirred solution of tert-butyl 2-(3-cyano-4-fluorophenyl)propanoate (360mg, 1.44mmol) from Step A and 3,4-dichloro-N-(4-hydroxyphenyl)benzamide (488 mg, l.73mmol) from Step B in DMSO (4ml) was added K ₂ CO ₃ (240 mg, 1.73 mmol). The reaction was stirred at 125 °C overnight, then cooled to ambient temperature and diluted with EtOAc and 10% aqueous sodium carbonate. The aqueous phase was extracted with EtOAc and the combined organics washed with 10% aqueous sodium carbonate and brine, dried over MgSO ₄ , concentrated and purified on a Biotage Horizon (40+M 5%to50% B:EtOAc, 21 ml-1008 ml). The appropriate fractions were then combined and concentrated to give tcrt-butyl 2-(3-cyano-4-(4-(3,4- dichlorobcnzamido)phenoxy)phenyl)propanoate (0.476 g, 0.797 mmol, 55%). ¹ H NMR (400 MHz, CDCl ₃ ) δ 7.99 (s, IH), 7.84 (bs, N-H), 7.72 (d, J=9.3 Hz, IH), 7.67 (d, J=8.7 Hz, 2H), 7.60 (m, 2H), 7.42 (d, J=8.6 Hz, IH), 7.11 (d, J=7.7, 2H), 6.83 (d, J=8.7 Hz, IH), 3.61 (q, J=7.1 Hz, IH), 1.45 (d, J=7.5 Hz, 3H), 1.42 (s, 9H).

[00212] Step D: To a stirred solution of tert-butyl 2-(3-cyano-4-(4-(3,4- dichlorobcnzamido)phenoxy)phenyl)propanoate (25 mg, 0.049 mmol) in DCM ( I ml) was added TFA (250 μl). The reaction was stirred at ambient temperature for 4 hours and then concentrated. The crude product was purified via preparative TLC (5%MeOH/DCM) to give 2-(3-cyano-4-(4-(3,4-

40 dichlorobenzamido)phcnoxy)phcnyl)propanoic acid (0.0174 g, 0.0382 mmol, 78%). ¹ H NMR (400 MHz, CDCI ₃ ): 8.04 (bs, N-H), 7.96 (s, IH), 7.64-7.71 (m, 3H), 7.61 (s, IH), 7.55 (d, J=7.7 Hz, IH) ₅ 7.44 (dd, J=8.6 _: 2.2 Hz, I H), 7.08 (d, J=8.9 Hz, 2H), 6.83 (d, J=8.5 Hz, 1 H), 3.74 (q, J=7.4 Hz, 1H) _: 1.53 (d, J=6.9 Hz, 3H).

Example 36 2-(4-(4-(3,4-diclilorobenzamido)phenoxy)-3-(methylsulfonamid omethyl)phenyl)propanoic acid

[00213] Prepared by the method of Example 14 substituting methyl 4-(4-(2-tert-butoxy-2- oxocthyl)-2-cyanophenoxy)bcnzoate in Step B with tert-butyl 2-(3-cyano-4-(4-(3,4- dichlorobcnzamido)phenoxy)phenyl)propanoatc and following steps C and F. ¹ H NMR (400 MHz, CDiOD): 8.10 (s, IH), 7.85 (s, IH), 7.60-7.70 (m, 3H), 7.50 (s, IH), 7.20 (d, IH), 7.1 (d, 2H), 6.80 (d, IH), 4.30 (s, 2H), 3.75 (q, IH), 2.80(2, 3H), 1.45 (d, 3H).

Example 37

2-(4-(4-(3,4-dichlorobenzamido)phenoxy)-3-(methylsulfonam idomethyl)phenyl)-2- methylpropanoic acid

[00214| Step A: To a stirred solution of NaH (60% in oil, 195 mg, 4.88 mmol) in DMF (10 ml) at O ⁰ C was added a solution of tert-butyl 2-(3-cyano-4-fluorophenyl)acetate (500 mg, 2.125 mmol) and McI (330 μl, 5.31mmol) in DMF (1 ml) dropwise. The reaction was warmed to ambient temperature, stirred overnight, and then diluted with IN HCl and EtOAc. The aqueous was extracted with EtOAc and the combined organics washed with brine and dried over MgSOφ The crude concentrated product was then purified on the Biotage Horizon (40+M 5%to 50% BiEtOAc, 18 ml- 864 ml) to give tert-butyl 2-(3-cyano-4-fIuorophenyl)-2-methylpropanoate (0.423 g, 1.602 mmol, 75%). ¹ H NMR (400 MHz, CDCI ₃ ) δ 7.56-7.63 (m, 2H), 7.17 (t, J=8.1 Hz, IH), 1.54 (s, 6H), 1.38 (s, 9H).

41

[002l5| Step B: 2-(4-(4-(3,4-Dichlorobenzamido)phenoxy)-3-(meλylsulfonamido methyl) phenyl)-2-methylpropanoic acid was prepared from tert-butyl 2-(3-cyano-4-fluorophenyl)-2τ methylpropanoate by the method of Example 35 substituting tert-butyl 2-(3-cyano-4- fluorophenyl)propanoatc in Step B with tert-butyl 2-(3-cyano-4-fluorophcnyl)-2-mcthylpropanoate and following steps B,C and F of Example 14. ¹ H NMR (400 MHz, CD ₃ OD): 8.15 (s, IH), 7.85 (d, IH) ₅ 7.65-7.75 (m, 3H), 7.55 (s, I H), 7.30 (d, IH), 7.05 (d, 2H), 6.80 (d, IH), 4.35 (s, 2H), 2.80 (s, 3H), 1.55 (s, 6H).

Example 38

2-(3-(Methylsulfonamidomethyl)-4-(4-(4- (trifluoromethyl)phenylcarbamoyl)phenoxy)phenyl)acetic acid

|00216] Prepared by the method of Example 14, substituting 3,4-dichlorobenzenamine in Step E with 4-trifluoromcthylbenzenamine. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.95 (d, 2H), 7.82 (d, 2H), 7.62 (d, IH), 7.45 (s, I H), 7.25 (d, IH), 7.05 (d, 2H), 6.95 (d, IH), 4.30 (s, 2H), 3.62 (s, 2H), 2.82 (s, 3H).

Example 39

2-(3-(MethyIsulfonamidomethyl)-4-(4-(3- (trifluoromethyl)ρhenylcarbamoyl)pheno ^χ y)phenyl)acetic acid

(00217) Prepared by the method of Example 14, substituting 3,4-dichlorobenzenamine in Step E with 3-trifluoromethylbenzenamine. ¹ H NMR (400 MHz, CD ₃ CI ₃ , CD ₃ OD) 8.05 (s, IH), 7.90 (m, 3H), 7.50 (m, IH), 7.45 (s, IH), 7.40 (m, I H), 7.25 (d, I H), 7.05 (d, 2H), 6.85 (d, IH), 4.30 (s, 2H), 3.65 (s, 2H) ₁ 2.85 (s, 3H).

42 Example 40

2-(4-(4-(4-chloro-3-fluorophenylcarbamoyl)phenoxy)-3- (methylsulfonamidomethyl)phenyl)acetic acid

(00218] Prepared by the method of Example 14, substituting 3,4-dichlorobenzenamine in Step E with 4-chloro-3-fluorobenzenamine. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.90 (d, 2H), 7.75 (m, IH), 7.30-7.45 (m, 3H), 7.25 (d, 1 H), 7.05 (d, 2H), 6.95 (d, I H), 4.3 (s, 2H), 3.65 (s, 2H), 2.85 (s, 3H).

Example 41

2-(4-(4-(3-chloro-4-fluoi*ophenylcnrban>oyl)phenoxy)-3 - (methylsulfonamidomethyl)phenyl)acetic acid

[00219] Prepared by the method of Example 14, substituting 3,4-dichlorobenzenamine in Step E with 3-chloro-4-fluorobenzenamine. 'H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.93 (d, 2H), 7.85 (m, IH), 7.55 (m, IH), 7.45 (s, IH), 7.25 (d, IH), 7. 15 (t, IH), 7.05 (d, 2H), 6.85 (d, IH), 4.30 (s, 2H), 3.65 (s, 2H) ₁ 2.85 (s, 3H).

Example 42

2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)phenyl)acetic acid

43

[00220| Step A: 2-(4-hydroxyphenyl)acetic acid ( 1.0 g, 6.57 mmol) was diluted with THF (4 mL) followed by the addition of Reactant 2 (2.63 g, 13.1 mmol). The reaction was stirred for hours 12 hours. The reaction was filtered and loaded directly onto a biolage 4OM cartridge eluting with hexancsxthyl acetate (3:1) to yield tert-butyl 2-(4-hydroxyphenyl)acctate (800 mg, 58.4% yield) as a clear oil that later solidified to a white solid.

[00221 ] Step B: 2-(4-chlorophenyl)ethanamine (0.321 ml, 2.27 mmol) was diluted with DCM (10 mL) followed by the addition of DIEA (d 0.742) (0.395 ml, 2.27 mmol) and 4-fluorobenzoyl chloride (0.231 ml, 1.89 mmol). After stirring for 30 minutes the reaction was loaded directly onto a biotage 4OS cartridge and eluted with hexanes:ethyl acetate (2: 1 ) to yield N-(4-chlorophenethyl)-4- fluorobenzamide (393 nig, 74.8% yield) as a white solid.

[00222] Step C: Tert-butyl 2-(4-hydroxyphenyl)acetate (200 mg, 0.960 mmol) was diluted with DMSO (4 mL) followed by the addition of K ₂ CO ₃ ( 133 mg, 0.960 mmol) and N-(4- ch!orophenethyl)-4-fluorobcnzamide (267 mg, 0.960 mmol). The reaction was stirred at 85 ⁰ C overnight, and then stirred at 138 ⁰ C for 12 hours. The reaction was cooled to ambient temperature, diluted with DCM and 10% aq. sodium carbonate. The layers were separated and the organic layer was dried over MgSO4, filtered and concentrated. The crude material was purified by silica gel chromatography, eluting with hexanesxthyl acetate (4: 1) to yield tert-butyl 2-(4-(4-((4- chlorophcnethyl)carbamoyl)phcnoxy)phenyl)acetate (20 mg, 4.47% yield) as an off white solid. [00223] Step D: Tert-butyl 2-(4-(4-((4-chlorophenethyl)carbamoyI)phenoxy)phenyl)acetate (20 mg, 0.043 mmol) was diluted with DCM (1 mL) followed by the addition of TFA (1 mL). After stirring for 2 hours, the reaction was concentrated, diluted with ether and concentrated to yield 2-(4- (4-((4-chlorophcnethyl)carbamoyl)phenoxy)phenyl)acetic acid (17 mg, 97% yield) as a white solid. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.70 (d, 2H), 7.30 (m, 4H), 7.19 (m, 2H), 7.00 (m, 4H), 3.62 (m, 4H), 2.90 (t, 2H).

Example 43 2-(4-(4-(4-chlorophenethylcarbamoyl)pheπoxy)-3-fluorophenyl )acetic acid

[00224] Step A: 2-(3-Fluoro-4-hydroxyphenyl)acetic acid (1.0 g, 5.88 mmol) was diluted with THF:McOH (3: 1) (10 mL) followed by the addition Of TMSCHN ₂ (5.88 ml, 11.8 mmol). After

44 stirring for 1 hour, the reaction was quenched with 2N HCl and diluted with DCM. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated. The material was purified by silica gel chromatography, cluting with hexanes:cthyl acetate (2: 1) to yield methyl 2-(3- fluoro-4-hydroxyphenyl)acetate (700 mg, 64.7% yield) as a clear oil.

[00225] Step B: 2-(4-Chlorophenyl)ethanamine (2.3 ml, 16 mmol) was diluted with DCM (40 niL) followed by the addition of DIEA (2.9 ml, 16 mmol) and 4-chloro-3-nitrobenzoyI chloride (3.0 g, 14 mmol) dropwise in 10 mL of DCM. After stirring for 30 minutes the reaction was loaded directly onto a silica gel column and eluted with hcxancsxthyl acetate (2: 1) to yield N-(4- chlorophcncthyl)-4-chloro-3-nitrobcnzamide (4.0 g, 86% yield) as a white solid. [00226] Step C: Methyl 2-(3-fluoro-4-hydroxyphenyl)acetate (prepared in Step A; 163 mg, 0.885 mmol) was diluted with DMSO (3 mL) followed by the addition of K ₂ CO ₃ ( 122 mg, 0.885 mmol) and N-(4-chlorophenethyl)-4-chloro-3-nilrobenzamide (prepared in Step B; 300 mg, 0.885 mmol). The reaction was stirred at 8O ⁰ C for 12 hours. The reaction was cooled, diluted with DCM and washed with 10% aq. sodium carbonate, water and brine. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated. The material was pun Tied by silica gel chromatography, eluting with hcxane: acetone (2: 1) to yield methyl 2-(4-(4-((4- chlorophenetliyI)carbamoyl)-2-nitrophenoxy)-3-fluorophenyl)a cetate (200 mg, 46.4% yield). |00227] Step D: Methyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)-2-nitrophenoxy)-3- fluorophenyl)acetate (100 mg, 0.205 mmol) was diluted with THF (2 mL) followed by the addition of Zn dust ( 13.4 mg, 0.205 mmol) and saturated aq. NH4C1 ( I mL). After stirring for 1 hour, the reaction was diluted with ethyl acetate and 10%aq sodium carbonate. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated to yield methyl 2-(4-(4-((4- chlorophenethyl)carbamoyl)-2-aminophenoxy)-3-fluorophenyl)ac etate (93 mg, 99.1% yield). [00228] Step E: Isobutyl nitrile (0.060 ml, 0.51 mmol) was diluted with DMF (2 mL), placed under nitrogen and heated to 6O ⁰ C followed by the addition of methyl 2-(4-(4-((4- chlorophenethyl)carbamoyI)-2-aminophenoxy)-3-fluorophenyl)ac etate (93 mg, 0.20 mmol) in 500 μL of DMF. The reaction was stirred 30 minutes and then cooled. The reaction was diluted with ethyl acetate and washed with 2N HCI and saturated sodium bicarbonate. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated. The material was purified by silica gel chromatography, eluling with hexancs:ethyl acetate (2: 1) to yield methyl 2-(4-(4-((4- chlorophenethyOcarbamoyOphenoxy^S-fluorophenyOacetate (35 mg, 39% yield). [00229] Step F: Methyl 2-(4-(4-((4-chlorophencthyl)carbamoyl)phenoxy)-3- fluorophenyl)acetate (32 mg, 0.072 mmol) was diluted with dioxane ( 1 mL) followed by the addition of NaOH (0.072 ml, 0.36 mmol) and 200 μL of water. After stirring for 3 hours, the reaction was

45 diluted with ethyl acetate and 2N HCl. The organic layer was dried over MgSO ₄ , filtered and concentrated to yield 2-(4-(4-((4-chlorophcnethyl)carbamoyl)phenoxy)-3-fluoropheny l)acetic acid (15 mg, 48% yield) as a white solid. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.70 (d, 2H), 7.25 (d, 2H), 7.19 (m, 3H), 7.10 (m, 2H), 6.95 (d, 2H), 3.65 (m, 4H), 2.90 (t, 2H).

Example 44 2-(3-cyano-4-(4-(4-chlorophenethylcarbamoyl)phenoxy)phenyl)a cetic acid

[00230] Step A: A flask was charged with tert-bulyl 2-(3-cyano-4-fluorophenyl)acetate (9.410 g, 40.00 mmol), methyl 4-hydroxybenzoate (7.303 g, 48.00 mmol), K ₃ CO ₃ (6.634 g, 48.00 mmol), and DMSO (160 mL). The mixture was heated to 90 ⁰ C under a nitrogen atmosphere for 17 hours. The mixture was cooled to ambient temperature and poured into a mixture of EtOAc (250 mL) and 10% K ₂ CO ₃ solution (250 mL). The resulting insoluble residue was dissolved in water and added to the EtOAc-aqueous mixture. The mixture was stirred for 1 hour and layers were separated. The aqueous layer was extracted with EtOAc, and the combined extracts were washed with saturated KjCO ₃ , water, and brine, dried over MgSO ₄ , filtered through a Celite pad, and concentrated under reduced pressure to give 14.7 g of crude product as an oil. The crude material was purified by silica gel chromatography to provide methyl 4-(4-(2-tert-butoxy-2-oxocthyl)-2-cyanophcnoxy)benzoate (11.6 g, 79%) as a white solid.

(00231] Step B: Methyl 4-(4-(2-tert-butoxy-2-oxoethyl)-2-cyanophenoxy)benzoate (1 1.60 g, 31.57 mmol) was dissolved in dioxane (160 mL) and the solution was cooled to 10 ⁰ C in an ice bath. LiOH-H ₂ O, IM (37.89 ml, 37.89 mmol) was added to the solution and the mixture was stirred at ambient temperature for 5 hours. The mixture was diluted with 2N HCl ( 10OmL) and CH ₂ Cb (200 mL). Layers were separated and aqueous layer was extracted with CH ₂ Cl ₂ . The combined extracts were washed with brine (100 mL), dried over MgSO ₄ , filtered through a Celite pad and concentrated under reduced pressure. The crude material was purified by silica gel chromatography to provide 4- (4-(2-tert-butoxy-2-oxoethyl)-2-cyanophenoxy)benzoic acid (7.29 g) as a white solid. [00232] Step C: To 4-(4-(2-tert-butoxy-2-oxocthyl)-2-cyanophenoxy)bcnzoic acid (0.34 g, 0.962 mmol), 2-(4-chlorophenyl)ethanamine (0.165 g, 1.06 mmol) in DMF (5 ml) was added diisopropylamine (0.200 ml, 1.15 mmol) and HBTU (0.149 g, 1.15 mmol). The reaction was stirred

46 al ambient temperature for 90 minutes. The reaction was diluted with water and the product extracted into ethyl acetate. The combined organic layers were dried (MgSOa), filtered and concentrated to give tert-butyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3- cyanophenyl)acetate (0.40 g, 85%) as a white solid after column chromatography. [00233] Step D: To tert-butyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3- cyanophenyOacetatc (4.40 g, 8.96 mmol) in DCM (20 ml) was treated with TFA (20 ml). After stirring for 2 hours. The reaction mixture was concentrated and the crude material was purified by silica gel chromatography using a gradient of 0.5% McOH/DCM containing 0.5% AcOH to 10% McOH/DCM containing 0.5% AcOH to provide 2-(4-(4-((4-chlorophencthyl)carbamoyl)phcnoxy)- 3-cyanophcnyl)acctic acid (3.30 g, 84.7% yield). ¹ H NMR (400 MHz, CD ₃ OD) 7.85 (d, 2H), 7.65 (s, IH), 7.45 (d, IH), 7.35 (s, IH), 7.25 (d, 2H), 7.20 (d, 2H), 7.10 (d, 2H), 6.95 (d, IH), 3.62 (m, 4H), 2.90 (t, 2H).

Example 45

2-(3-cyano-4-(4-(2,4-dichlorophenethylcarbamoyl)phenoxy)p henyl)acetic acid

[00234] Prepared by the method of Example 44 substituting 2-(2,4-dichlorophcnyl)ethanamine for 2-(4-chlorophcnyl)cthanaminc in Step D. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.79 (d, 2H), 7.65 (m, IH), 7.45 (d, IH), 7.40 (m, IH), 7.21 (m, 2H), 7.10 (d, 2H), 6.85 (d, IH), 3.65 (m. 4H), 3.05 (t, 2H).

Example 46 2-(3-bromo-4-(4-(2,4-dichIorophenethylcarbamoyl)phenoxy)phen yl)acetic acid

[002351 Step A: N-(4-chlorophenethyl)-4-hydroxybenzamide ( 1.5 g, 5.4 mmol) was diluted with DMSO (15 mL) followed by the addition of K ₂ CO ₃ (0.89 g, 6.4 mmol) and 3-bromo-4-

47 fluorobenzaldehydc (1.0 g. 4.9 mmol). The reaction was stirred at 85 ⁰ C overnight and then cooled to ambient temperature. The reaction mixture was diluted with ethyl acetate and washed with twice with 10% sodium carbonate, water and brine, and then concentrated. The crude material was purified by silica gel chromatography eluting with hexanes:ethyl acetate (1 :1) and then 100% ethyl acetate to yield N-(4-chlorophcnethyl)-4-(2-bromo-4-formylphenoxy)benzamide (1.6 g, 71% yield). [00236] Step B: N-(4-chlorophenethyl)-4-(2-bromo-4-formylphenox>')benzami de (1.6 g, 3.5 mmol) was diluted with THF (10 mL) followed by the addition of N,N,N- trimcthyl(phenyi)methanaminiurn hydroxide (0.79 ml, 1.7 mmol) and mcthylsulfinyl(rnethylthio)methane (0.87 g, 7.0 mmol). The reaction was stirred at 70 ⁰ C for 2 hours. The reaction mixture was loaded directly onto a silica gel column and clutcd with DCM:MeOH (98:2) to yield (Z)-N-(4.chlorophcncthyl)-4-(2-bromo-4-(2-(mcthylsulfinyl)-2 - (methyithio)vinyl)phenoxy)benzamide (1.9 g, 96% yield).

[00237] Step C: (Z)-N-(4-chlorophencthyl)-4-(2-bromo-4-(2-(methylsulfinyl)-2 -(methyithio) vinyl)phcnoxy)benzamide (2.0 g, 3.5 mmol) was treated with HCI (8.9 ml, 18 mmol) in ethanol. The reaction was stirred at 70 ⁰ C for 2 hours. The reaction was cooled and loaded directly onto a silica gel column eluting with hexanes:cthyl acetate (3: 1) to yield ethyl 2-(4-(4-((4- chlorophenethyl)carbamoyl)phenoxy)-3-bromophenyl)acetate.

|00238] Step D: Ethyl 2-(4-(4-((4-chlorophencthyl)carbamoyl)phcnoxy)-3-bromophenyl )acetate (30 ing, 0.058 mmol) was diluted with dioxane (500 μL) followed by the addition of NaOH (0.093 ml, 0.46 mmol) and 200 μL of water. After stirring for 3 hours, the reaction was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated to yield 2-(4-(4-((4-chlorophenethyl)carbaιnoyl)phenoxy)-3-bromophen yl)acetic acid (24 mg, 85% yield) as a white solid. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.70 (d, 2H), 7.60 (s, IH), 7.22-7.32 (m, 3H), 7.19 (d, 2H), 7.05 (d, IH), 6.90 (d, 2H), 3.62 (m, 4H), 2.90 (l, 2H).

Example 47

2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)3,5-dimethylp henyl)acetic acid

[00239] Prepared by the method of Example 46 substituting 4-fluoro-3,5-dimethylbcnzaldehyde for 3-bromo-4-fluorobenzaldehyde in step A. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.60 (d, 2H),

48

7.25 (d, 2H), 7.15 (d, 2H), 7.05 (s, 2H), 6.78 (d, 2H), 3.65 (t, 2H), 3.58 (s, 2H), 2.90 (t, 2H), 2.15 (s, 6H).

Example 48 2-(4-(4-(4-chIorophenethylcarbamoyl)phenoxy)3-methylphenyl)a cetic acid

[00240] Step A: Ethyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)pheno\y)-3-bromophenyl )acetate (Example 46 step C ; 30 mg, 0.058 mmol) was diluted with THF ( 1 mL) followed by the addition of bis(tri-t-butγlphosphinc)palladium (0) (3.0 mg, 0.0058 mmol) and methylzinc chloride (0.087 ml, 0.17 mmol). After stirring for 2 hours, the reaction was loaded directly onto a silica gel column, cluting with hexanesxthyl acetate (3:1) to yield ethyl 2-(4-(4-((4- chlorophcncthyl)carbarnoyl)phcnoxy)-3-mcthylphcnyl)acetatc (20 mg, 76% yield) as a clear oil. (00241] Step B: Ethyl 2-(4-(4-((4-chlorophcnetliyl)carbamoyl)phcnoxy)-3-mcthylphen yl)acetate (20 mg, 0.0443 mmol) was diluted with dioxane (500 μL) followed by the addition of NaOH (0.0885 ml, 0.443 mmol) and 200 μL of water. After stirring for 3 hours, the reaction was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSO.), filtered and concentrated to yield 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3- mcthylphcnyOacctic acid (16.0 mg, 85.3% yield). ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.62 (d, 2H), 7.30 (d, 2H), 7.10-7.22 (m, 4H), 6.92 (d, IH), 6.85 (d, 2H), 3.65 (t, 2H), 3.60 (s, 2H), 2.90 (U 2H), 2.15 (s, 3H).

Example 49

2-(4-(4-(4-chlorophenethylcarbamoyl)plienoxy)3-(thiophen- 2-yl)phenyl)acetic acid

49

[00242| Step A: Ethyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3-bromophenyl )acetate (Example 46 step C; 24 mg, 0.046 mmol) was diluted with THF (1 mL) followed by the addition of bis(tri-t-butylphosphine)palladium (0) (2.4 mg, 0.0046 mmol) and 2-thienylzinc bromide (0.23 ml, 0.12 mmol). After stirring for 2 hours, the reaction was purified by silica gel chromatography, cluting with hexanes:ethyl acetate (3: 1) to yield ethyl 2-(4-(4-((4-chlorophenethyl) carbamoy])phcnoxy)-3-(thiophen-2-yl)phenyl)acetate (20 mg, 83% yield) as a clear oil. [00243] Step B: Ethyl 2-(4-(4-((4-chlorophencthyl)carbamoyl)phenoxy)-3-(thiophen-2 - yl)phenyl)acctate (20 mg, 0.038 mmol) was diluted with dioxane (500 μL) followed by the addition of NaOH (0.052 ml, 0.26 mmol) and 200 μL of water. After stirring for 3 hours, the reaction was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSO.>, filtered and concentrated to yield 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3- (thiophen-2-yl)phenyl)acetic acid (7 mg, 37% yield) as a white solid. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.69 (s, IH), 7.64 (d, 2H), 7.45 (d, IH), 7.30 (d, 2H), 7.22 (d, IH), 7.15 (d, 2H), 6.98-7.05 (m, 2H), 6.90 (d, 2H), 3.65 (m, 4H) ₁ 2.90 (t, 2H).

Example 50 2-(6-(4-(4-chlorophenethylcarbamoyl)phenoxy)biphenyl-3-yl)ac etic acid

[00244] Step A: Ethyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phcnoxy)-3-bromophcnyl )acctate (Example 46 step C; 24 mg, 0.046 mmol) was diluted with THF (1 mL) followed by the addition of bis(tri-l-butylphosphine)palladium (0) (2.4 mg, 0.0046 mmol) and phenylzinc iodide (0.23 ml, 0.12 mmol). After stirring for 2 hours, the reaction was loaded directly onto a silica gel column and eluted with hexanesiethyl acetate (3: 1) to yield Ethyl 2-(6-(4-(4- chlorophcnethylcarbamoyl)phenoxy)biphenyl-3-yl)acetate (15 mg, 63% yield) as a clear oil. [00245] Step B: Ethyl 2-(6-(4-(4-chlorophenethylcarbamoyl)phcnoxy)biphenyl-3-yl)ac etate (10 mg, 0.0195 mmol) was diluted with dioxane (1 mL) followed by the addition of NaOH (0.0389 ml, 0.195 mmol) and 200 μL of water. After stirring for 2 hours, the reaction was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated to yield 2-(6-(4-(4-chlorophenethylcarbamoyl)phenoxy)biphenyl-3-yl)ac etic acid (1.6 mg, 16.9% yield) as a white solid. MS ESl negative M-H = 485.

50

Example 51 2-(6-(4-(4-chIorophenethylcarbamoyl)phenoxy)-3'-(methylsulfo nyl)biphenyl-3-yl)acetic acid

[00246] Step A. Ethyl 2-(4-(4-((4-chlorophcnethyl)carbamoyl)phenoxγ)-3-bromopheny I)acetate (Example 46 step C; 100 mg. 0.193 mmol), 3-(mcthanesulfonyl)phenylboronic acid (58.1 mg, 0.290 mmol), K ₂ CO ₃ (80.2 mg, 0.580 mmol) and Pd(PPh ₃ ) ₄ (22.4 mg, 0.0193 mmol) were diluted with dioxane (2 mL) and water (1 mL). The reaction was purged three times with nitrogen and stirred at 60 ⁰ C overnight. The reaction was cooled and loaded directly onto a silica gel column eluting with hexanes:ethyl acetate (2: 1) to yield ethyl 2-(6-(4-(4-chlorophenethyIcarbamoyl)phenoxy)-3'- (methylsulfonyl)biphenyl-3-yl)acetate (80 mg, 69.8% yield).

(00247) Step B: Ethyl 2-(6-(4-(4-chlorophcnethylcarbamoyl)phenoxy)-3'-

(methylsulfonyl)biphenyl-3-yl)acetate (80 mg, 0.135 mmol) was diluted with dioxane (1 mL) followed by the addition of NaOH (0.270 ml, 1.35 mmol) and 300 μL of water. After stirring for 2 hours, the reaction was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated The material was purified using a biotage 12i cartridge eluting with 1-4% methanol/DCM to yield 2-(6-(4-(4- chlorophenclhylcarbamoyl)phcnoxy)-3'-(mcthylsulfonyl)biphcny l-3-yl)acetic acid (5 mg, 6.56% yield). ¹ H NMR (400 MHz, CD ₃ Cb, CD ₃ OD) 8.05 (s, IH), 7.81 (d, IH), 7.78 (d, IH), 7.50-7.60 (m, 3H), 7.42 (s, I H), 7.38 (d, IH), 7.25 (d, 2H), 7.19 (d, 2H), 7.05 (d, IH), 6.85 (d, IH), 3.75 (s, 2H), 3.62 (q, 2H), 3.0 (s, 3H), 2.90 (t, 2H).

Example 52

2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-cyclopropy Iphenyl)acetic acid

51

|00248] Step A: A flask was charged with cyclopropyl magnesium bromide (29.6 ml, 14.8 mmol) and placed under nitrogen. Zinc chloride ( 14.8 ml, 14.8 mmol) was added and the reaction was stirred for 20 minutes. 3-Bromo-4-fluorobenzaldehyde (1.0 g, 4.93 mmol) and bis(tri-t- butylphosphinc)palladium (0) (0.126 g, 0.246 mmol) were diluted in 600 μL of THF and added to the reaction mixture. After stirring for 4 hours, the reaction was heated to 5O ⁰ C and stirred overnight. The reaction was cooled and quenched with saturated NH ₄ Cl and extracted with DCM. The organic layer was dried over MgSO ₄ , filtered and concentrated. The material was purified using a biotagc 4OM cartridge cluting with hcxanesxthyl acetate (4: 1) to yield 3-cyclopropyl-4- fluorobenzaldchyde (400 mg, 49.5% yield)

[00249] Step B: N-(4-Chlorophenethyl)-4-hydroxybenzamide (504 mg, 1.83 mmol) was diluted with DMSO (8 mL) and K ₂ CO ₃ (379 mg, 2.74 mmol) and 3-cyclopropyl-4-fluorobenza!dehyde (300 mg, 1.83 mmol) were added. The reaction was stirred 85 ⁰ C overnight. The reaction was cooled to ambient temperature, diluted with ethyl acetate and washed with twice with 10% sodium carbonate, water and brine, and concentrated. The crude material was purified by silica gel chromatography eluting with hexanes.ethyl acetate (3: 1) to provide N-(4-chlorophenethyl)-4-(2-cyclopropyl-4- formylphcnoxy)benzamide (160 mg, 20.9% yield).

|002501 Step C: N-(4-Chlorophenethyl)-4-(2-cyclopropyl-4-formylphenoxy)benza mide (160 mg, 0.381 mmol) was diluted with THF (3 mL) followed by the addition of N,N,N- trirnethyl(phenyl)mcthanaminium hydroxide (0.0866 ml, 0.191 mmol) and methylsulfinyl(mcthy!thio)rnethane (94.7 mg, 0.762 mmol). The reaction was heated to 70 ⁰ C and stirred for 2 hours. The reaction was cooled and loaded directly onto a silica gel column, eluting with ethyl acetate to yield (Z)-N-(4-chloropheneUiyl)-4-(2-cyclopropyl-4-(2-(methylsulf� �nyl)-2- (methylthio)vinyl)phenoxy)benzamide (100 mg, 49.9% yield).

1002511 Step D: (Z)-N-(4-chIorophenetliyl)-4-(2-cyclopropyl-4-(2-(methylsulf inyl)-2-

(methylthio)vinyl)phenoxy)benzamide (100 mg, 0.190 mmol) was diluted with HCl (0.950 ml, 1.90 mmol), heated to 7O ⁰ C and stirred for 4 hours. The reaction was cooled and loaded directly onto a biotage 25 cartridge and eluted with hexanes:ethyl acetate (3: 1) to yield ethyl 2-(4-(4-((4- chlorophenethyl)carbamoyl)phcnoxy)-3-cyclopropylphcnyl)accta tc (70 mg, 77.0% yield) as a white solid.

[00252] Step E: Ethyl 2-(4-(4-((4-chlorophcncthyl)carbamoyl)phcnoxy)-3- cyclopropylphenyl)acetate (70 mg, 0.146 mmol) was diluted with dioxane (1 mL) followed by the addition of NaOH (0.293 ml, 1 ,46 mmol) and water (300 μL). After stirring for 3 hours, the reaction was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated to yield 2-(4-(4-((4-chlorophenethyl)

52 carbamoyl)phenoxy)-3-cyclopropylphenyl)acetic acid (50 mg, 75.9% yield) as a white solid. ¹ H NMR (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.65 (d _s 2H), 7.30 (d, 2H), 7.15 (d, 2H), 7.10 (d, IH), 6.90 (m, 4H), 3.65 (t, 2H), 3.60 (s, 2H), 2.00 (t, 2H) _; 2.05 (m, I H), 0.80 (m, 2H), 0.62 (m, 2H).

Example 53 2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-ethylphenyl)a cetic acid

|00253] Step A: Ethyl 2-(4-(4-((4<hlorophencthyl)carbamoyl)phenoxy)-3-bromophen yl)acetate (Example 46 step C; 30 mg, 0.058 mmol) was diluted with THF (1 mL) followed by the addition of l, r-bis(diphcnylphosphino)ferroccncdichloropalladium(II) (4.8 mg, 0.0058 mmol) and diethylzinc (0. 13 ml, 0.15 mmol). After stirring for 4 hours, the reaction loaded directly onto a silica gel column, eluϋng with 5-50% ethyl acetate/hexane to yield ethyl 2-(4-(4-((4- chlorophcnethyl)carbamoyl)phenoxy)-3-ethylphcnyl)acctate (10 mg, 37% yield). [00254] Step B: Elhyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3-ethylphenyl )acetate (10 mg, 0.0215 mmol) was diluted with dioxanc (500 μL) followed by the addition of NaOH (0.0429 ml, 0.215 mmol) and 5 drops of water. After stirring for 3 hours, the reaction was diluted with ethyl acetate and 2N HCl, and the layers were separated. The organic layer was dried over MgSO.1, filtered and concentrated. The crude material was purified by silica gel chromatography eluting with 10%MeOH/DCM to yield 2-(4-(4-((4-chlorophencthyl)carbamoyl)phcnoxy)-3- ethylphenyOacetic acid (2 mg, 21.3% yield). (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.65 (d, 2H), 7.30 (d, 2H), 7.10-7.25 (m, 4H), 6.90 (m, 3H), 3.62 (m, 2H), 3.40 (s, IH), 2.90 (t, 2H), 2.55 (q, 2H), 1.15 (t, 3H).

Example 54 2-(6-(4-(4-chIorophenethylcarbamoyl)phenoxy)-4'-(methylsulfo nyl)biphenyl-3-yl)acetic acid

53

[00255] Step A: Ethyl 2-(4-(4-((4-chlorophcnctliyl)carbamoyl)phenoxy)-3-bromopheny l)acctate (Example 46 step C; 40 mg, 0.077 mmol), K ₂ CO ₃ (32 mg, 0.23 mmol) and 4- (methylsulfonyl)phenylboronic acid (23 mg, 0.12 mmol) were diluted with dioxane (1 mL)/water (300 μL) followed by the addition of Pd(PPh ₃ ) ₄ (8.9 mg, 0.0077 mmol). The reaction was purged with nitrogen and stirred at 55°C for 12 hours. The reaction was cooled and concentrated. The residue was taken up in minimal DCM and purified by silica gel chromatography, eluting with hexanes:ethyl acetate ( 1 : 1) to yield ethyl 2-(6-(4-(4-chlorophencthylcarbamoyI)phenoxy)-4'- (methylsulfonyl)biphenyl-3-yl)acetate (20 mg, 44% yield) as a clear oil.

[00256] Step B: Ethyl 2-(6-(4-(4-chlorophenethylcarbamoyl)phenoxy)-4'-

(methylsulfonyl)biphenyl-3-yl)acetate (20 mg, 0.0338 mmol) was diluted with dioxane ( 1 mL) followed by the addition of NaOH (0.0676 ml, 0.338 mmol) and 300 μL of water. After stirring for 3 hours, Uic reaction was diluted with ethyl acetate and 2N HCl. The layers were separated and the organic layer was dried over MgSO ₄ , filtered and concentrated to yield 2-(6-(4-(4- chlorophenethylcarbamoyl)phenoxy)-4'-(methylsulfonyl)bipheny l-3-yl)acetic acid (10 mg, 52.5% yield). (400 MHz, CD ₃ Cl ₃ , CD ₃ OD) 7.90 (d, 2H), 7.70 (d, 2H), 7.60 (d, 2H), 7.40 (s, IH), 7.35 (d, IH), 7.25 (d, 2H), 7.15 (d, 2H), 7.05 (d, I H), 6.85 (d, 2H), 6.55 (t, IH), 3.63 (m, 4H), 3.05 (s, 3H), 2.90 (t, 2H).

Example 55

2-(3-(methylsulfonamidomethyl)-4-(4-(3- (trifluoromethyl)phenethylcarbamoyl)phenoxy)phenyl)acetic acid

[00257] Prepared by the method of Example 14, substituting 3,4-dichlorobcnzenamine in Step E with 2-(3-trifluoromethylphenyI)ethyanamine. MS+ 551.1 [M + I].

54 Example 56

2-(3-(methylsulfonamidomethyl)-4-(4-(4- trifluoromethyl)phenethylcarbamoyl)phenoxy)phenyl)acetic acid

[00258| Prepared by ϋie rnelliod of Example 14, substituting 3,4-dichlorobenzenainine in Step E with 2-(4-trifluoromethylphenyl)ethyanamine. MS+ 550.9 [M + I].

Example 57

2-(4-(4-((l-(4-chlorophenyl)cyclopropyl)methylcarbamoyl)p henoxy)-3- (methylsulfonamidomethyl)phenyl)acetic acid

[00259] Prepared by the method of Example 14, substituting 3.4-dichlorobenzenamine in Step E with (l-(4-chlorophenyl)cycloproyl)methanamine. MS - 541.1 [M - 1].

Example 58 2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-(nicotinamido methyl)phenyl)acetic acid

[00260] Step A: A mixture of te/7-butyl 2-(3-cyano-4-fluorophenyl)acetate (1.32 g, 5.59 mmol), N-(4-chlorophenethyl)-4-hydroxybenzamide ( 1.85 g, 671 mmol), and potassium carbonate (0.93 g, 6.70 mmol) in DMSO (10 ml) was stirred at 90 ⁰ C for 1 day. The reaction was cooled, diluted with ethyl acetate, and washed with 10% aqueous NazCC^. The aqueous layer was back extracted with

55 ethyl acetate. The organic washes were combined, washed with 10% NajCOs and brine, dried over sodium sulfate and concentrated. The crude material was purified by silica gel chromatography, cluting with a gradient of 5% ethyl acetatc/hexanes to 60% ethyl acetatc/hexanes gave /er/-butyl 2- (4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3-cyanophenyl)a cetate (1.09 g, 40%). (00261] Step B: To tert-Butyl 2-(4-(4-((4-chlorophencthyl)carbamoyl)phenoxy)-3- cyanophenyl)acetate ( 1.09 g.. 2.22 mmol) in 6 ml of 7 N ammonia in methanol at ambient temperature was added Raney-Ni (0.019 g) and the reaction was stirred under a balloon of hydrogen gas at ambient temperature. The reaction was filtered and concentrated to give /er/-butyl 2-(4-(4- ((4-chlorophcnethyl)carbamoyl)phcnoxy)-3-(aminomcthyl)phenyl )acctate (0. 160 g, 15%). [00262] Step C: A mixture of ter/-butyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3- (aminomethyl)phenyl)acetale (0.015 g, 0.030 mmol), nicotinoyl chloride hydrochloride (0.007 g, 0.039 mmol), and triethylamine (0.013 ml, 0.091 mmol) in dichloromethane (1 ml) was stirred at ambient temperature for 1 hour. The reaction was loaded onto a silica gel samplet and the product was eluted using a gradient system of 0.5% methanol/ dichloromethane to 5% methanol/dichloromethanc to provide tert-butyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3- (nicotinamidomethyl)phenyl)acetate (0.005 g, 275%).

|00263| Step D: To a solution of /«?r/-butyl 2-(4-(4-((4-chlorophencthyI)carbamoyl)phenoxy)-3- (nicotinamidomethyl)phenyl)acctate (0.005 g, 0.008 mmol) in dichloromethane (1.0 ml) was added TFA (1.0 ml) and the reaction was stirred at ambient temperature. After 1 hour the reaction was concentrated to give 2-(4-(4-((4-chlorophenethyl)carbamoyl)phcnoxy)-3-(nicotinami domethyl) phenyOacctic acid (0.005 g). MS -449.1 [M - CO2H],

Example 59

2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-((pyridine -3- sulfonamido)niethyl)phenyl)acetic acid

(00264] Prepared by the method of Example 58, substituting nicotinoyl chloride hydrochloride in Step C with pyridine-3-sulfonyl chloride hydrochloride. MS -578.1 [M - 1].

Example 60

2-(4-(4-(4-chlυroplienethylcarbamoyl)plieπυxy)-3-((l-m ethyl-l//-imidazole-5- sulfonamido)methyl)phenyl)acetic acid

56

[00265] Prepared by the method of Example 58, substituting nicotinoyl chloride hydrochloride in Step C with 1 -methyl- 1 H-imidazole-4-sulfonyl chloride. MS -581.2 [M - I].

Example 61

2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-((6- dimethylamino)nicotinamido)methyl)phenyl)acetic acid

[00266] Prepared by the method of Example 58, substituting nicotinoyl chloride hydrochloride in Step C with 6-(dimethylamino)nicotinic acid. MS -585.2 [M - 1].

Example 62

2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-((2-(4- fluorophenylsulfonamido)acetamido)methyl)phenyl)acetic acid

[00267] Prepared by the method of Example 58, substituting nicotinoyl chloride hydrochloride in Step C with 2-(4-fluorophenylsulfonamido)acetic acid. MS -608.4 [M - CO2H].

Example 63

2-(3-((6-aminonicotinamido)methyl)-4-(4-(4- chloroplienethylcarbamoyl)phenoxy)phenyl)acetic acid

57

[00268] Prepared by the method of Example 58, substituting nicotinoyl chloride hydrochloride in Step C with 6-aminonicotinic acid. MS -557.1 [M - 1].

Example 64 2-(4-(4-(4-chloi ophenethycnrbnmoyl)phenoxy)-3-((dimethylnminno)methyl)phenyl )acetic acid

|00269] Step A: To a stirred solution of tert-butyl 2-(3-(aminonicthyl)-4-(4- (phcnethylcarbamoyl)phenoxy)phenyl)acetatc (0.015 g, 0.030 mmol) in nictlianol (1 ml) was added acetic acid (0.012 ml, 0.212 mmol), sodium cyanoborohydridc (0.010 g, 0.152 mmol) and paraformaldehyde (0.007 g, 0.152 mmol). The reaction was stirred at ambient temperature for 1 hour. The reaction was loaded directly onto a silica gel samplct and the product elutcd using a gradient of 0.5% mcthanol/dichloromcthanc to 5% methanol/ dichloromcthanc to provide /er/-butyl 2-(4-(4-((4-chloroplienetliyl)carbamoyl)phenoxy)-3-((dimelhy lamino)methyl)phcnyl)acetate (0.005 g, 0.010 mmol).

[00270] Step B: To tert-bulyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3-

((dimethylamino)methyl)phenyl)acetate (0.005 g, 0.010 mmol) in dichloromethane (1.0 ml) was added TFA (1.0 ml) and the reaction stirred for 1 hour at ambient temperature. The reaction was concentrated to give 2-(4-(4-((4-chlorophenethyl)carbamoyI)phenoxy)-3-

((dimethylamino)mcthyl)phenyl)acctic acid (0.005 g). MS -465.1 [M - I ].

Example 65

2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-((N- methylmethylsulfonamido)methyl)phenyl)acetic acid

58

(00271) Step A: To a stirred solution of tert-buty\ 2-(3-cyano-4-fluorophcnyl)acetatc (1.00 mg, 4.25 mmol) and methyl 4-hydroxybenzoate (776 mg, 5.10 mmol) in DMSO (20 ml) was added K ₂ CO ₃ (704 mg, 5.10 mmol). The reaction was heated to 90 ⁰ C overnight via an oil bath. The reaction was diluted with ethyl acetate and 10% aqueous sodium carbonate. The aqueous phase was extracted with ethyl acetate and the combined organic layers were washed with 10% aqueous sodium carbonate and brine, dried over MgSO ₄ , concentrated and purified by silica gel chromatography to give methyl 4-(4-(2-ftτv-butoxy-2-oxoethyl)-2-cyanophenoxy)benzoate (1.08 g). [00272] Step B: To a stirred solution of the methyl 4-(4-(2-κ?rt-buloxy-2-oxoethyl)-2- cyanophcnoxy)bcnzoatc (1.086 mg, 2.96 mmol) in 7N ammonia/mcthanol (30 ml) under nitrogen was added Raney Nickel (25.3 mg, 0.296 mmol). The reaction was purged with a hydrogen balloon and stirred under hydrogen overnight. The reaction was filtered through GF paper and the solid catalyst was rinsed with methanol and ethyl acetate. The filtrate was concentrated, and the crude product was purified by silica gel chromatography to give methyl 4-(2-(aminomethyl)-4-(2-/e«- bιιtoxy-2-oxoethyl)phcnoxy)bcnzoate (0.69 g).

(00273) Step C: To a stirred solution of the methyl 4-(2-(aminomcthyl)-4-(2-re«-butoxy-2- oxoethyl)phenoxy)benzoate (690 mg, 1.86 mmol) in dichloromethane (6 ml) was added pyridine (225 μL, 2.78 mmol) followed by mclhanesulfonyl chloride (287 μL, 3.71 mmol). The reaction was stirred at ambient temperature for 4 hours, after which time additional pyridine (1.5 eq) and mcthanesulfonyl chloride (2 eq) were added. The reaction was stirred for an additional hour and then diluted with ethyl acetate and IM HCI. The aqueous layer was extracted with ethyl acetate and the combined organics washed with brine and dried over MgSO ₄ . The crude product was then purified on the Biotagc Horizon (40+M, 5%to75% B:ethyl acetate) to give methyl 4-(4-(2-/e/7-butoxy-2- oxoethyl)-2-(methylsulfonamidomethyl)phenoxy)benzoate (0.754 g).

(00274) Step D: To a stirred solution of the methyl 4-(4-(2-fm-butoxy-2-oxoethyl)-2- (mcthylsulfonamidomethyl)phenoxy)benzoate (745 mg, 1.65 mmol) in DMF (10 ml) was added McI ( 155 μL, 2.48 mmol) followed by K2CO3 (343 mg, 2.48 mmol). The reaction was stirred at ambient temperature for 3 hours, then diluted with ethyl acetate and 2M HCI. The aqueous phase was extracted with ethyl acetate, and the combined organic layers were washed with brine and dried

59 over MgS0.». The crude mixture was purified by silica gel chromatography to give methyl 4-(4-(2- /m-butoxy-2-oxoethyl)-2-((N-meλyImeώan-2-ylsulfonamido)met hyl)phenoxy)benzoate (0.368 g). |00275] Step E: To a stirred solution of methyl 4-(4-(2-fcτf-butoxy-2-oxoethyl)-2-((N- mcthyImethan-2-ylsulfonarnido)methyl)phenoxy)benzoate (370 mg, 0.797 mmol) in dioxane (8 ml) was added a solution of LiOH monohydratc (43.5 nig, 1.04 mmol) in water (2 ml) (0.1 M solution 4: 1 dioxane/watcr). The reaction was stirred for 5 hours, then diluted with dichloromethane and 2N HCl. The aqueous phase was extracted with dichloromethane and the combined organic layers were washed with brine and dried over MgSQi. The crude product was purified by silica gel chromatography to give 4-(4-(2-tert-butoxy-2-oxocthyl)-2-((N-mcthylmethan-2- ylsulfonamido)mcthyl)phcnoxy)bcnzoic acid (0.253 g).

[00276] Step F: To a stirred solution of 4-(4-(2-λ?/?-butoxy-2-oxoelhyl)-2-((N-methylmethan-2- ylsulfonamido)methyl)phcnoxy)benzoic acid (50 mg, 0.11 1 mmol) and HATU (46.5 mg, 0.122 mmol) in DMF (1 ml) was added DIEA (23.2 μL, 0.133 mmol) followed by 2-(4- chlorophenyl)cthanamine (11.2 μL, 0.11 1 mmol). The reaction was diluted with water and extracted with ethyl acetate. The combined organics were washed with brine and dried over MgSCj. The crude product was purified by silica gel chromatography to give /e«-butyl 2-(4-(4-((4- chlorophencthyl)carbamoyl)phcnoxy)-3-((N-methylmethan-2-ylsu lfonamido)mcthyl)phenyl)acetate (0.0115 g).

100277] Step G: To a stirred solution of tert-butyl 2-(4-(4-((4- chlorophcnethyl)carbamoyl)phcnoxy)-3-((N-methylmcthan-2-ylsu lfonamido)methyl)phcnyl)acctate (1 1.5mg, 0.019mmol) in dichloromethane (ImI) was added TFA (50OuI). The reaction was stirred at ambient temperature for 4 hours and then concentrated.. The crude product was then purified by preparative TLC (20% methanol/dichloromethane/0.5% AcOH) to give 2-(4-(4-((4- chlorophencthyl)carbamoyl)phcnoxy)-3-((N-methylmethan-2-ylsu lfonamido)methyI)phenyl)acetic acid (0.0123 g). MS +531.0 [M + I].

Example 66 2-(3-cyano-4-(4-(phenethylcarbamoyl)phenoxy)phenyl)acetic acid

60

[00278] Step A: A mixture of /ert-butyl 2-(3-cyano-4-fluorophenyl)acetate (0.122 g), N-(4- chlorophenethyl)-4-hydroxybcnzamide (0.150 g), and potassium carbonate (0.086 g) was dissolved in 2 ml of DMSO and stirred at 90 ⁰ C. After 1 day, the reaction was cooled, loaded onto a silica gel samplct and product eluted with a solvent system gradient of 0.5% methanol/ dichloromethane to 5% methanol/ dichloromethane to give /er/-butyl 2-(3-cyano-4-(4-

(phcncthylcarbamoyl)phenoxy)phenyl)acctate (0.120 g).

(00279) Step B: To a solution of tert-butyl 2-(3-cyano-4-(4-(phcncthylcarbamoyl) phenoxy)phenyl)acctate 0. 120 g) in 2 ml of dichloromethane was added 2 ml of TFA and the reaction was stirred at ambient temperature. After 1 hour the reaction was concentrated and dried under vacuum to give 2-(3-cyaiio-4-(4-(phenethylcarbamoyl)phenoxy)phcnyl)acctic acid (0.100 g). MS -355.3 [M - CO2H].

Example 67 2-(4-(4-(4-chlorophenethyIcarbamoyl)-3-(trifluoromethyl)phen yl)acetic acid

[00280] Step A: To a stirred solution of the 4-fluoro-3-(trifluoromethyl)benzaldehyde (177 μL, 1.30mmol) and N-(4-chlorophenethyl)-4-hydroxybcnzamide (359 mg, 1.30 mmol) in DMSO (3 ml) was added K ₂ CO ₃ (270 mg, 1.95 tnmol). The reaction was stirred at 95°C for 2 hours and Chen directly purified by silica gel chromatography to give N-(4-chlorophenethyl)-4-(4-formyl-2- (trifluoromethyl)phenoxy)benzamide (0.541 g).

[00281] Step B: To a solution of N-(4-chlorophenethyl)-4-(4-formyl-2-

(trifluoromcthyl)phenoxy)benzamide (0.225 g, 0.502 mmol) in THF (5 ml) was added methylsulfinyl(methylthio)methane (0.125 g, 1.00 mmol) followed by benzyltrimethylammonium hydroxide (0.114 ml, 0.251 mmol) and the reaction was stirred at 70 ⁰ C forl hour. The reaction was cooled, loaded onto a silica gel samplet and the product was eluted using a gradient of 0.5% methanol/dichloromethane to 15% methanol/dichloromethane. Isolated (Z)-N-(4-chlorophenethyl)- 4-(4-(2-(meUiylsulfinyl)-2-(methylthio)vinyl)-2-(trifluorome thyl)phenoxy)benzamide (0.220 g, 79.0% yield) which by TLC appeared to be a 4: 1 mixture of olefin isomers. The crude material was used directly in the next step.

61

(002821 Step C: To (Z)-N-(4-chlorophenethyl)-4-(4-(2-(mεthylsulfinyl)-2-(melhy lthio)vinyl)-2- (trifluoromethyl)phenoxy)bcnzamide (0.220 g, 0.397 mmol) was added HCl (in ethanol) (0.993 ml, 1.99 mmol) and the reaction heated to 7O ⁰ C for 1 hour. The reaction was concentrated, loaded onto silica gel and the product eluted using a gradient of 5% ethyl acetate/hexanes to 75% ethyl acetate/hexanes. Isolated ethyl 2-(4-(4-((4-chlorophenethyl)carbamoyI)phenoxy)-3-

(trifluoromethyl)phenyl)acetate (0.165 g, 82.1% yield) as a white solid.

[00283] Step D: To a solution of ethyl 2-(4-(4-((4-chIorophcnethyl)carbamoyl)phenoxy)-3- (trifluoromethyl)phenyl)acetate (0. 165 g, 0.326 mmol) in methanol/THF (1: 1), 5 ml was added LiOH-H ₂ O (0.0684 g, 1.63 mmol) and 3 drops of water, and the reaction was stirred for 1 hour The reaction was diluted with ethyl acetate, washed with 2N HCl and brine, dried over magnesium sulfate and concentrated. The crude material was purified by silica gel chromatography, eluting with a gradient of 0.5% methanol/ dichloromethane containing 0.5% AcOH to 7% methanol/ dichloromethane containing 0.5% AcOH to provide 2-(4-(4-((4-chlorophenethyl) carbamoyl)pheno\y)-3-(trifluoromethyl)phenyl)acetic acid (0.112 g). MS +478.3 [M + I]. ¹ H NMR (400 MHx, CD ₃ OD) δ 8.49 (bt, NH), 7.79 (d, J = 8.7 Hz, 2H), 7.68 (s, I H), 7.53 (d, J - 4.3 Hz, IH), 7.28 (d, J = 8.5 Hz, 2H), 7.23 (d, J = 8.6 Hz, 2H), 7.02-7.06 (m, 3H), 3.71 (s, 2H), 3.56 (t, J = 6.8 Hz, 2H), 2.90 (t, J = 7.3 Hz, 2H).

Example 68 2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-cyanophenyl)p ropanoic acid

[00284] Step A: To a stirred solution of diisopropylamine in 2 ml of THF at -78 ⁰ C was added n- butyl lithium (0.61 ml). The reaction was stirred for 30 minutes after which time a solution of tert- butyl 2-(3-cyano-4-fluorophenyl)acetate (0.300 g) in THF (2 ml) was added dropwise over 20 minutes at -78 ⁰ C. The reaction was warmed to 0 ⁰ C and then transferred a flask containing 2 ml THF and MeI (0.080 ml). The reaction was acidified with 2M HCl and extracted with ethyl acetate. The combined organics were then washed with brine and dried over MgSO ₄ . The concentrated product was purified by silica gel chromatography using a solvent system of 5% to 50% ethyl acetate/hexanes to provide re/7-butyl 2-(3-cyano-4-fluorophenyl)propanoate (0.140g).

62

[00285| Step B: A flask was charged with ten-butyl 2-(3-cyano-4-fluorophenyl)acetate (0.070 g), N-(4-chlorophenethyl)-4-hydroxybcnzamide (0.105 g), and potassium carbonate (0.047 g) in 1 ml of DMSO and stirred at 95 ⁰ C for one day. The reaction was taken up in dichloromcthanc and washed with water. The organic layer was collected, dried over MgSCU filtered, and concentrated. The crude material was purified by silica gel chromatography, eluting with a solvent system of 5%- 80% ethyl acetate/hexanes gave /cw-butyl 2-(4-(4-((2,4-dichlorophenethyl)carbamoyl)phenoxy)-3- cyanophcnyOpropanoate (0.102).

(00286) Step C: A flask was charged with λ?r/-butyl 2-(4-(4-((4- chlorophenethyl)carbamoyl)phenoxy)-3-cyanophenyl)propanoate (0.098 g) in 1 ml of dichloromethane and 1 ml of TFA, and the reaction was stirred for 1 hour. The reaction was concentrated and dried under vacuum. Isolated 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3- cyanophenyOpropanoic acid (0.080 g). MS +449.1 [M +1J.

Example 69 2-(3-cyano-4-(2,4-dichlorophenethylcarbamoyl)phenoxy)phenyl) propanoic acid

[00287] Prepared by the method of Example 68 substituting N-(4-chlorophenethyl)-4- hydroxybenzamidc in step B with N-(2,4-dichlorophenelhyl)-4-hydroxybenzarnidc. MS +483.0 [M

+1J-

Example 70 2-(4-(4-(4-chlorophenethyIcarbamoyl)-3-methoxyphenyl)acetic acid

[00288) Step A: Methyl 2-(4-hydroxy-3-mctho\yphenyl)acetate (0.204 g, 1.04 mmol), N-(4- chlorophcncthyl)-4-iodobenzamidc (0.200 g, 0.519 mmol), 2,2,6,6-tetramethyl-3,5-heptanedionc

63

(0.00956 g, 0.0519 mmol), Cu(I)Cl (0.0257 g, 0.259 mmol) and Cs ₂ CO ₃ (0.338 g, 1.04 mmol) were stirred together in NMP (2 ml) for 1 hour. The reaction was loaded onto silica gel and the product elulcd using a gradient of 5% ethyl acetat^exancs to 100% ethyl acetate/hexancs. Isolated methyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3-methoxyphen yl)acetate (0.105 g, 44.6% yield) as a yellow solid.

[00289} Step B: To a solution of methyl 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3- methoxyphenyl)acetate (0.065 g, 0.143 mmol) in methanol/THF (1: 1, 3 ml) was added NaOH (0.100 ml, 0.500 mmol). After 5 minutes, reaction was quenched with 10 ml of 2N HCl and the product was extracted into ethyl acetate. The organic layer was washed with brine, dried over magnesium sulfate and concentrated. The crude material was purified by silica gel chromatography, cluting with a gradient of 0.5% methanol/dichloromcthane (containing 0.5% AcOH) to 7.5% methanol/dichloromethane containing (0.5% AcOH) to provide 2-(4-(4-((4- chlorophenethyI)carbamoyl)phenoxy)-3-mcthoxyphcnyl)acctic acid (0.041 g, 65.1% yield) as a white solid. ¹ H NMR (400 MHz, CD ₃ OD) δ 8.37-8.41 (bt, NH), 7.70 (d, J = 8.7 Hz, 2H), 7.28 (d, J = 8.6 Hz, 2H), 7.23 (d, J = 8.5 Hz ₅ 2H), 7.08 (s, IH), 7.02 (d, J - 8.6 Hz, IH), 6.91 (d, J = 8.6 Hz, 2H), 3.64 (s, 2H), 3.56 (t, , J = 6.8 Hz, 2H), 2.88 (t, J = 7.4 Hz, 2H).

Example 71 2-(3-cyano-4-(4-(2,4-dichloi ophenethylcarbamoyl)-3-fluoι ophenoxy)phenyl)acetic acid

(002901 Step A: To a stirred solution of 2-fluoro-4-hydroxybenzoic acid (200mg, 1.28mmol) in DMF (3 ml) at ambient temperature were added HATU (536 mg, 1.41 mmol) and DIEA (268 μL, 1.54 mmol). The reaction was stirred for 30 minutes and then 2-(2,4-dichlorophenyl)ethanamine (193 μL, 1.28 mmol) was added. The reaction was stirred overnight, then concentrated and diluted with water and dichloromethane. The aqueous layer was extracted with dichloromethane and the combined organic layers were washed with brine and dried over MgSO4. The crude product was purified by silica gel chromatography) to give N-(2,4-dichlorophenelhyl)-2-fluoro-4- hydroxybcnzamide (0.420 g).

[00291] Step B: To a stirred solution of N-(2,4-dichlorophenethyl)-2-fluoro-4- hydroxybenzamide (420 mg, 1.28 mmol) and rm-butyl 2-(3-cyano-4-fluorophenyl)acetate (361 mg,

64

1.54 mmol) in DMSO (6 ml) was added K ₂ CO ₃ (265 mg, 1.92 mmol). The reaction was heated stirred at 7O ⁰ C for one hour. The reaction was then stirred at 9O ⁰ C overnight. The reaction was diluted with water and ethyl acetate. The aqueous phase was extracted with ethyl acetate and the combined organic layers were washed with 10% Na^CO ₃ and brine, dried over MgSO ₄ , filtered and concentrated. The crude product was purified by silica gel chromatography to give /e/7-butyl 2-(4- (4-((2,4-diclilorophenethyl)carbamoyl)-3-fluorophenoxy )-3-cyanophcny Oacetate (0.0157 g). [00292] Step C: To a stirred solution of /e«-butyl 2-(4-(4-((2,4-dichlorophenethyl)carbamoyl)-3- fluorophenoxy)-3-cyanophenyl)acetatc (15 mg, 0.0276 mmol) in dichloromcthanc was added TFA. The reaction was stirred for 2 hours and then concentrated. The crude material was purified by preparative TLC ( 10%mcthanol/0.5%AcOH/dichloromcthanc). The appropriate section was collected and then purified a second time by preparative TLC to provide 2-(4-(4-((2,4- dichlorophcnethyl)carbamoyl)-3-fluorophenoxy)-3-cyanophenyl) acetic acid (0.0042 g). ¹ H NMR (400 MHz, CD ₃ OD) δ 7.70-7.74 (m, 2H), 7.61 (d, J = 7.8 Hz, IH), 7.43 (s, IH), 7.33 (d, J = 8.6 Hz, IH), 7.26 (d, J = 7.8 Hz, IH), 7.13 (d, J = 8.7 Hz, IH), 6.88-6.94 (m, 2H), 3.52-3.65 (m, 4H), 3.05 (t, J = 7.0 Hz, 2H).

Example 72 2-(3-cyano-4-(4-(2,6-dichlorophenethylcarbamoyl)phenoxy)phen yl)acetic acid

(00293) Step A: TFP Resin ( 1.33 mmol/g; 2.872 g, 3.820 mmol) was placed in a vessel and DMA (5 mL) was added to swell the resin. The vessel was placed on a shaker for 15 minutes, and 4-(4-(2-/e/v-butoxy-2-oxoethyl)-2-cyanophenoxy)benzoic acid (1.500 g, 4.245 mmol), DIC (0.7311 ml, 4.669 mmol), and DMAP (0.5704 g, 4.669 mmol) were added to the mixture and the vessel was placed on the shaker. The mixture was filtered, washed with DMA, THF, dichloromethane, and ether, and air-dried to give 3.1 1 g of the resin bound (4-(4-(2-fert-buloxy-2-oxoethyl)-2- cyanophcnoxy)ben/:oate.

[00294] Step B: TFP resin bound ester (4-(4-(2-tørt-butoxy-2-oxoethyl)-2- cyanophenoxy)benzoate) (0.1968 g, 0.09840 mmol) was added to a vial. THF (2 ml) was added and the resin was allowed to swell. 2-(2,6-Dichlorophenyl)ethanamine, (IM in THF; 0.08200 ml, 0.082 mmol) was added to the vial and the vial was place on a shaker. After 16 hours, the mixture was

65 decanted and the resin was rinsed with THF (3 X 2ml). The combined THF solution was concentrated under reduced pressure. The residue was dried under high vacuum to give fen-butyl 2- (4-(4-((2,6-dichlorophcncthyl)carbamoyl)phenoxy)-3-cyanophcn yl)acetatc (0.034 g), which was used in the nest step without further purification.

[00295) Step C: tert-Butyl 2-(4-(4-((2,6-dichlorophenethyl)carbamoyl)phenoxy)-3- cyanophcnyl)acetate (34 mg, 0.065 mmol) was dissolved in dichloromethane (ImI) and TFA (ImI) was added. After stirring for 45 minutes, the mixture was concentrated under reduced pressure to give 2-(3-cyano-4-(4-(2,6-dichlorophencthyIcarbamoyl)phenoxy)phen yl)acctic acid (0.034 g).

Example 73 2-(3-cyano-4-(4-(4-fluorophenethylcarbamoyl)phenoxy)phenyl)a cetic acid

[00296] Prepared by the method of Example 72 substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with 2-(4-fluorophenyl)ethanamine.

Example 74

2-(3-cyano-4-(4-(3-methoxyphenethylcarbamoyl)phenoxy)phen yl)acetic acid

[00297] Prepared by the method of Example 72 substituting 2-(2,6-dtchlorophenyl)ethanamine in Step B with 2-(3-melhoxyphenyl)cthanaminc.

Example 75 2-(3-cyano-4-(4-(4-terf-butyIphenethylcarbamoyl)phenoxy)phen yl)acetic acid

66

[00298J Prepared by the method of Example 72 substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with 2-(4-tørt-butylphenyl)cthanaminc.

Example 76 2-(3-cyano-4-(4-(4-trifluoromethylphenethylcarbamoyl)pheιι oxy)phenyl)acetic acid

(00299| Prepared by the method of Example 72 substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with 2-(4-trifluoromethylphcnyl)ethanamine.

Example 77 2-(3-cyano-4-(4-(3-chlorophenethylcarbamoyl)pheno ^χ y)phenyl)acetic acid

[00300] Prepared by the method of Example 72 substituting 2-(2,6-dich!orophenyl)ethanaminc in Step B with 2-(3-chiorophcnyl)ethanamine.

Example 78 2-(3-cyano-4-(4-(3-fluorophenethylcarbamoyl)phenoxy)phenyl)a cetic acid

67

[00301] Prepared by the mclhod of Example 72 substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with 2-(3-fluorophenyl)cthanamine.

Example 79 2-(3-cyano-4-(4-(4-methoxyphenethylcarbamoyl)phenoxy)phenyl) acetic acid

[00302] Prepared by the method of Example 72, substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with 2-(4-methoxyphenyl)ethanamine.

Example 80

2-(3-cyano-4-(4-(4-methylphenethylcarbamoyl)phenoxy)pheny l)acetic acid

[00303] Prepared by tlie method of Example 72, substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with 2-(4-methylphenyl)ethanamine

Example 81 2-(3-cyano-4-(4-(4-methylphenethylcarbamoyl)phenoxy)phenyl)a cetic acid

68

[00304J Prepared by the method of Example 72, substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with 2-(3,4-dichlorophenyi)ethanamine.

Example 82 2-(4-(4-(2-(4-chlorophenyl)-2-hydroxyethyIcarbamoyl)phenoxy) -3-cyanophenyl)acetic acid

[00305| Prepared by the method of Example 72 substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with 2-amino- l-(4-chlorophenyl)cthanol hydrochloride.

Example 83 2-(4-(4-(l-(4-chlorophenyl)cyclopropylcarbamoyl)phenoxy)-3-c yanophenyl)acetic acid

[00306] Prepared by the method of Example 72 substituting 2-(2,6-dichlorophenyl)cthanamine in Step B with l-(4-chlorophenyl)cycloproanamine hydrochloride.

Example 84 2-(3-cyano-4-(4-(2-phenylcyclopropylcarbamoyl)phenoxy)phenyl )acetic acid

69

[00307] Prepared by the method of Example 72 substituting 2-(2,6-dichlorophcnyl)ethanamine in Step B with 2-phenyIcyclopropylaminc.

Example 85 2-(4-(4-(4-chlorophenethylcarbamoyl)phenoxy)-3-cyanophenyl)- 2-fIuoroacetic acid

[00308] Step A: tert-Butyl 2-(4-(4-(4-chlorophenethylcarbamoyl)phcnoxy)-3-cyanophenyl) acetate (0.400 g) and 4-methylbenzencsulfonyl azide (0.193 g) were dissolved in 5 ml of acetonitrile and treated with DBU (0.152 ml) The reaction was stirred for 4 hours, then concentrated, diluted with ethyl acetate and washed with water. The organic layer was dried, filtered, concentrated onto silica gel and purified by silica gel chromatography to provide tert-butyl 2-(4-(4-(4- chlorophenethylcarbamoyI)phenoxy)-3-cyanophenyl)-2-diazoacel ate (0.48 g) as a bright yellow solid.

100309] Step B: A flask was charged with /er/-butyl 2-(4-(4-(4- chlorophenethylcarbamoyl)phenoxy)-3-cyanophenyl)-2-diazoacet ate (0.108 g), 5 ml of ether and HF pyridine (0.30 g). The reaction was stirred at 40 ⁰ C for 30 minutes. The reaction mixture was diluted with ethyl acetate and neutralized with saturated aqueous bicarbonate solution. The organic layer was separated and washed with brine, dried, filtered and concentrated onto silica gel. The crude material was purified by silica gel chromatography to provide ten-butyl 2-(4-(4-((4- chlorophencthyl)carbamoyl)phenoxy)-3-cyanophenyl)-2-fluoroac etate (0.026 g) as a colorless oil. [00310] Step C: A flask was charged with rm-butyl 2-(4-(4-((4- chlorophencthyl)carbamoyl)phenoxy)-3-cyanophenyl)-2-fluoroac etate (0.026 g), 0.5 ml of dichloromcthanc and 0.2 ml of TFA. The reaction was concentrated, and the residue was dissolved

70 in a minimal amount of dichloromethane and placed under high vacuum to give a solid. The procedure was repeated to give 2-(4-(4-((4-chlorophenethyl)carbamoyl)phenoxy)-3-cyanophenyl )-2- flυoroacetic acid (0.024 g) as a tan solid.

Example 86 2-(4-(4-((l-(4-chloιophenyl)cyclopropyl)methylcarbamoyl)phe noxy)-3-cyanophenyl)acetic acid

[00311 ] Prepared by the method of Example 72 substituting 2-(2,6-dichlorophenyl)ethanamine in Step B with (l-(4-chlorophneyl)cyclopropyl)mcthanaminc.

Example 87 2-(4-(4-(2-(4-chlorophenyl)cyclopropylcarbamoyl)phenoxy)-3-c yanophenyl)acetic acid

[00312] Prepared by the method of Example 72 substituting 2-(2,6-dichlorophcnyl)ethanamine in Step B with 2-(4-chlorophenyl)cyclopropanamine.

Example 88 2-(3-cyano-4-(4-(l,2,3,4-tetrahydronaphthalen-2-ylcarbamoyl) phenoxy)phenyl)acetic acid

[00313] Prepared by the method of Example 72 substituting 2-(2,6-dichlorophcnyl)ethanamine in Step B with l,2,3,4-tetrahydronaphthalen-2-amine hydrochloride.

71

Example 89

2-(3-cyano-4-(4-(6-rnethoxy-l,2,3,4-tetrahydronaphthalen- 2- ylcarbamoyl)phenoxy)phenyl)acetic acid

[00314] Prepared by the method of Example 72 substituting 2-(2,6-dichlorophcnyl)ethanamine in Step B with 6-methoxy-l,2,3,4-tetrahydronaphthalen-2-amine hydrochloride.