Title:
5-FORMYLCYTOSINE SPECIFIC CHEMICAL LABELING METHOD AND RELATED APPLICATIONS
Document Type and Number:
WIPO Patent Application WO/2015/043493
Kind Code:
A1
Abstract:
The present invention relates to a 5-formylcytosine specific chemical labeling method and related applications in aspects such as sequencing, detection, imaging, and diagnosis. In the method, a condensation reaction occurs between an active methylene group in an active methylene compound containing a side-chain reactive group and an aldehyde group in 5-formylcytosine or a 1-substituted derivative of 5-formylcytosine, and at the same time an intramolecular reaction occurs between the side-chain reactive group of the active methylene compound and a 4-amino group of cytosine to implement ring closing. By means of the 5-formylcytosine specific chemical labeling method and related compounds of the present invention, detection of the content of 5-formylcytosine in nucleic acid molecules, and specific concentration of 5-formylcytosine-containing nucleic acid samples, and analysis of sequence distribution information of 5-formylcytosine and/or single-base resolution sequence information in nucleic acid molecules and the like may be implemented. The present invention provides various effective research methods in the research fields of epigenetics and nucleic acid biochemistry.
Inventors:
YI CHENGQI (CN)
XIA BO (CN)
ZHOU ANKUN (CN)
XIA BO (CN)
ZHOU ANKUN (CN)
Application Number:
PCT/CN2014/087479
Publication Date:
April 02, 2015
Filing Date:
September 26, 2014
Export Citation:
Assignee:
UNIV BEIJING (CN)
International Classes:
C07C247/10; C07H21/04; C07H1/00; C09K11/06; C12Q1/68; G01N21/64
Foreign References:
| JPH09263584A | 1997-10-07 |
Other References:
MAMTA TAHILIANI ET AL., SCIENCE, vol. 324, 2009, pages 931 - 935
SKIRMANTAS KRIAUCIONIS; NATHANIEL HEINTZ, SCIENCE, vol. 324, 2009, pages 929 - 930
TONI PFAFFENEDER ET AL., ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 123, 2011, pages 7146 - 7150
SHINSUKE ITO ET AL., SCIENCE, vol. 333, 2011, pages 1300 - 1303
YUFEI HE ET AL., SCIENCE, vol. 333, 2011, pages 1303 - 1307
MICHAEL J.BOOTH ET AL., SCIENCE, vol. 336, 2012, pages 934 - 937
CHUNXIAO SONG ET AL., CELL, vol. 153, 2011, pages 678 - 691
ADAM B. ROBERTSON ET AL., NUCLEIC ACIDS RESEARCH, vol. 39, 2011, pages E55
WILLIAM A. PASTOR ET AL., NATURE, vol. 473, 2011, pages 394 - 397
CHUNXIAO SONG ET AL., NATURE METHODS, vol. 9, 2012, pages 75 - 77
MICHAEL J. BOOTH ET AL., SCIENCE, vol. 336, 2012, pages 934 - 937
MIAO YU ET AL., CELL, vol. 149, 2012, pages 1368 - 1380
EUN-ANG RAIBER ET AL., GENOME BIOLOGY, vol. 13, 2012, pages R69
LI SHEN ET AL., CELL, vol. 153, 2013, pages 692 - 706
CHUNXIAO SONG ET AL., CELL, vol. 153, 2013, pages 678 - 691
MICHAEL J. BOOTH ET AL., NATURE CHEMISTRY, vol. 6, 2014, pages 435 - 440
JIANLIN HU ET AL., CHEMISTRY-A EUROPEAN JOURNAL, vol. 19, 2013, pages 2013 - 5840
MICHAEL J.BOOTH ET AL., NATURE CHEMISTRY, vol. 6, 2014, pages 2014 - 440
LIANG ZHANG ET AL., JOURNAL OF AMERICAN CHEMICAL SOCIETY, vol. 136, 2014, pages 4801 - 4804
See also references of EP 3061764A4
SKIRMANTAS KRIAUCIONIS; NATHANIEL HEINTZ, SCIENCE, vol. 324, 2009, pages 929 - 930
TONI PFAFFENEDER ET AL., ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 123, 2011, pages 7146 - 7150
SHINSUKE ITO ET AL., SCIENCE, vol. 333, 2011, pages 1300 - 1303
YUFEI HE ET AL., SCIENCE, vol. 333, 2011, pages 1303 - 1307
MICHAEL J.BOOTH ET AL., SCIENCE, vol. 336, 2012, pages 934 - 937
CHUNXIAO SONG ET AL., CELL, vol. 153, 2011, pages 678 - 691
ADAM B. ROBERTSON ET AL., NUCLEIC ACIDS RESEARCH, vol. 39, 2011, pages E55
WILLIAM A. PASTOR ET AL., NATURE, vol. 473, 2011, pages 394 - 397
CHUNXIAO SONG ET AL., NATURE METHODS, vol. 9, 2012, pages 75 - 77
MICHAEL J. BOOTH ET AL., SCIENCE, vol. 336, 2012, pages 934 - 937
MIAO YU ET AL., CELL, vol. 149, 2012, pages 1368 - 1380
EUN-ANG RAIBER ET AL., GENOME BIOLOGY, vol. 13, 2012, pages R69
LI SHEN ET AL., CELL, vol. 153, 2013, pages 692 - 706
CHUNXIAO SONG ET AL., CELL, vol. 153, 2013, pages 678 - 691
MICHAEL J. BOOTH ET AL., NATURE CHEMISTRY, vol. 6, 2014, pages 435 - 440
JIANLIN HU ET AL., CHEMISTRY-A EUROPEAN JOURNAL, vol. 19, 2013, pages 2013 - 5840
MICHAEL J.BOOTH ET AL., NATURE CHEMISTRY, vol. 6, 2014, pages 2014 - 440
LIANG ZHANG ET AL., JOURNAL OF AMERICAN CHEMICAL SOCIETY, vol. 136, 2014, pages 4801 - 4804
See also references of EP 3061764A4
Attorney, Agent or Firm:
BEIJING WANXIANGXINYUE INTELLECTUAL PROPERTY OFFICE (CN)
北京万象新悦知识产权代理事务所（普通合伙） (CN)
北京万象新悦知识产权代理事务所（普通合伙） (CN)
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