Title:
ACID-RESISTANT YEAST STRAIN FOR HIGH-YIELD PRODUCTION OF SUCCINIC ACID, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/093796
Kind Code:
A1
Abstract:
Provided are a genetically modified yeast strain for producing succinic acid, which strain has the activity or an enhanced activity of an NADPH-dependent malate dehydrogenase (EC1.1.1.82), and optionally also has the activity or an enhanced activity of at least one of the following: (i) a soluble fumarate reductase (EC 4.2.1.2), (ii) a pyruvate carboxylase (EC 6.4.1.1), (iii) a fumarase (EC 4.2.1.2), and (iv) a succinic acid transporter; and a preparation method therefor, a method for producing succinic acid by using same, and the use thereof.
Inventors:
ZHANG XUELI (CN)
FAN FEIYU (CN)
XI YONGYAN (CN)
XU HONGTAO (CN)
FAN FEIYU (CN)
XI YONGYAN (CN)
XU HONGTAO (CN)
Application Number:
PCT/CN2022/133973
Publication Date:
June 01, 2023
Filing Date:
November 24, 2022
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N1/21; C12P7/46; C12R1/85
Foreign References:
| CN111295446A | 2020-06-16 | |||
| CN104694449A | 2015-06-10 | |||
| CN104178443A | 2014-12-03 |
Other References:
ZHANG TING, GE CHENGYU, DENG LI, TAN TIANWEI, WANG FANG: "C4-dicarboxylic acid production by overexpressing the reductive TCA pathway", FEMS MICROBIOLOGY LETTERS, vol. 362, no. 9, 1 May 2015 (2015-05-01), XP093068739, DOI: 10.1093/femsle/fnv052
SANCHEZ, A. M. ET AL.: "Efficient succinic acid production from glucose through overexpression of pyruvate carboxylase in an Escherichia coli alcohol dehydrogenase and lactate dehydrogenase mutant", BIOTECHNOLOGY PROGRESS, vol. 21, no. 2, 2 March 2005 (2005-03-02), pages 358 - 365, XP008136355, DOI: 10.1021/bp049676e
ZHANG YU-XIU, WANG JIAO, WANG DAN, QI FENG: "Advancement in Genetic Engineering for Production of Succinic Acid by Escherichia coli", CHINA BIOTECHNOLOGY., vol. 29, no. 7, 15 July 2009 (2009-07-15), pages 108 - 117, XP093068746, DOI: 10.13523/j.cb.20090719
姜振楠 (JIANG, ZHENNAN): "羧酸转运蛋白工程改造解脂耶氏酵母高效生产丁二酸 (Transporter Engineering in Yarrowia Lipolytica for High-efficient Succinic Acid Production)", 中国优秀硕士学位论文全文数据库(工程科技I辑) (ENGINEERING SCIENCE & TECHNOLOGY I, CHINA MASTER’S THESES FULL-TEXT DATABASE), 15 September 2021 (2021-09-15)
CHAE T UAHN J HKO Y S ET AL.: "Metabolic engineering for the production of dicarboxylic acids and diamines [J].", METABOLIC ENGINEERING,, 2019
HYOHAK SONGSANG YUP LEE.: "Production of Succinic Acid by Bacterial Fermentation [J].", ENZYME AND MICROBIAL TECHNOLOGY, vol. 39, no. 3, 2006, pages 352 - 361
AHN J HJANG Y SLEE S Y, PRODUCTION OF SUCCINIC ACID BY METABOLICALLY ENGINEERED MICROORGANISMS [J]. CURRENT OPINION IN BIOTECHNOLOGY, vol. 42, 2016, pages 54 - 66
SINGLETON ET AL.: "DICTIONARY OF MICROBIOLOGY AND MOLECULAR BIOLOGY", 1994, J. WILEY & SONS
SAMBROOK ET AL.: "MOLECULAR CLONING, A LABORATORY MANUAL", 1989, COLD SPRINGS HARBOR PRESS
NEEDLEMAN ET AL., J. MOL. BIOL., vol. 48, 1970, pages 443
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
SANCHEZ, A. M. ET AL.: "Efficient succinic acid production from glucose through overexpression of pyruvate carboxylase in an Escherichia coli alcohol dehydrogenase and lactate dehydrogenase mutant", BIOTECHNOLOGY PROGRESS, vol. 21, no. 2, 2 March 2005 (2005-03-02), pages 358 - 365, XP008136355, DOI: 10.1021/bp049676e
ZHANG YU-XIU, WANG JIAO, WANG DAN, QI FENG: "Advancement in Genetic Engineering for Production of Succinic Acid by Escherichia coli", CHINA BIOTECHNOLOGY., vol. 29, no. 7, 15 July 2009 (2009-07-15), pages 108 - 117, XP093068746, DOI: 10.13523/j.cb.20090719
姜振楠 (JIANG, ZHENNAN): "羧酸转运蛋白工程改造解脂耶氏酵母高效生产丁二酸 (Transporter Engineering in Yarrowia Lipolytica for High-efficient Succinic Acid Production)", 中国优秀硕士学位论文全文数据库(工程科技I辑) (ENGINEERING SCIENCE & TECHNOLOGY I, CHINA MASTER’S THESES FULL-TEXT DATABASE), 15 September 2021 (2021-09-15)
CHAE T UAHN J HKO Y S ET AL.: "Metabolic engineering for the production of dicarboxylic acids and diamines [J].", METABOLIC ENGINEERING,, 2019
HYOHAK SONGSANG YUP LEE.: "Production of Succinic Acid by Bacterial Fermentation [J].", ENZYME AND MICROBIAL TECHNOLOGY, vol. 39, no. 3, 2006, pages 352 - 361
AHN J HJANG Y SLEE S Y, PRODUCTION OF SUCCINIC ACID BY METABOLICALLY ENGINEERED MICROORGANISMS [J]. CURRENT OPINION IN BIOTECHNOLOGY, vol. 42, 2016, pages 54 - 66
SINGLETON ET AL.: "DICTIONARY OF MICROBIOLOGY AND MOLECULAR BIOLOGY", 1994, J. WILEY & SONS
SAMBROOK ET AL.: "MOLECULAR CLONING, A LABORATORY MANUAL", 1989, COLD SPRINGS HARBOR PRESS
NEEDLEMAN ET AL., J. MOL. BIOL., vol. 48, 1970, pages 443
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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