Title:
ACYL COENZYME A: CHOLESTEROL ACYLTRANSFERASE (ACAT)
Document Type and Number:
WIPO Patent Application WO1994009126
Kind Code:
A3
Abstract:
This invention pertains to purified, biologically active acyl coenzyme A: cholesterol acyltransferase (ACAT) and to nucleic acid (DNA or RNA) encoding acyl coenzyme A: cholesterol acyltransferase. The nucleic acid, or a fragment thereof, may be ligated with an expression vector and transfected into cells to express acyl coenzyme A: cholesterol acyltransferase activity in intact cells and in cell-free extracts.
Inventors:
CHANG TA-YWAN
CHANG CATHERINE C Y
CHANG CATHERINE C Y
Application Number:
PCT/US1993/009704
Publication Date:
June 23, 1994
Filing Date:
October 12, 1993
Export Citation:
Assignee:
DARTMOUTH COLLEGE (US)
International Classes:
C12N9/10; C12N15/54; C12N15/85; A61K38/00; (IPC1-7): C12N15/00; A01K67/027; C12N9/10; C12N15/11; C12N15/54; C12Q1/68
Other References:
K.M. KADIGAN ET AL.: "Isolation of chinese hamster ovary cell lines expressing human acyl-coenzyme A/cholesterol acyltransferase activity", J. CELL BIOLOGY, vol. 108, no. 6, June 1989 (1989-06-01), ROCKEFELLER UNIV. PRESS, N.Y. , US;, pages 2201 - 2210
D.J. JOLLY ET AL.: "Isolation of a genomic clone partially encoding human hypoxanthine phosphoribosyltransferase", PROC. NATL. ACAD SCI., vol. 79, no. 16, August 1982 (1982-08-01), NATL. ACAD SCI., WASHINGTON, DC, US;, pages 5038 - 5041
K.M. CADIGAN ET AL.: "Isolation and characterization of chinese hamster ovary cell mutants deficient in acyl-coenzyme A: cholesterol acyltransferase activity", J. BIOL. CHEM., vol. 263, no. 1, 5 January 1988 (1988-01-05), AM. SOC. MOL. BIOL., INC.,BALTIMORE, US;, pages 274 - 282
P.M. KINNUNEN ET AL.: "Chemical modification of acyl-CoA: cholesterol O-acyltransferase. Identification of acyl-CoA: cholesterol O-acyltransferase subtypes by differential diethyl pyrocarbonate sensitivity", BIOCHEMISTRY, vol. 27, no. 19, 20 September 1988 (1988-09-20), AM. CHEM. SOC.,WASHINGTON,DC,US;, pages 7344 - 7350
C.C. CHANG AND T.Y. CHANG: "Molecular cloning and functional expression of human acyl coenzyme A: cholesterol acyltransferase (ACAT) cDNA", FASEB JOURNAL, vol. 7, no. 7, 10 April 1993 (1993-04-10), ROCKEVILLE PIKE,BETHESDA, MD, US;, pages A1147
C.C.Y. CHANG ET AL.: "Molecular cloning and functional expression of human acyl-coenzyme A: cholesterol acyltransferase cDNA in mutant chinese hamster ovary cells", J. BIOL. CHEM., vol. 268, no. 28, 5 October 1993 (1993-10-05), AM. SOC. MOL. BIOL., INC.,BALTIMORE, US;, pages 20747 - 20755
D.J. JOLLY ET AL.: "Isolation of a genomic clone partially encoding human hypoxanthine phosphoribosyltransferase", PROC. NATL. ACAD SCI., vol. 79, no. 16, August 1982 (1982-08-01), NATL. ACAD SCI., WASHINGTON, DC, US;, pages 5038 - 5041
K.M. CADIGAN ET AL.: "Isolation and characterization of chinese hamster ovary cell mutants deficient in acyl-coenzyme A: cholesterol acyltransferase activity", J. BIOL. CHEM., vol. 263, no. 1, 5 January 1988 (1988-01-05), AM. SOC. MOL. BIOL., INC.,BALTIMORE, US;, pages 274 - 282
P.M. KINNUNEN ET AL.: "Chemical modification of acyl-CoA: cholesterol O-acyltransferase. Identification of acyl-CoA: cholesterol O-acyltransferase subtypes by differential diethyl pyrocarbonate sensitivity", BIOCHEMISTRY, vol. 27, no. 19, 20 September 1988 (1988-09-20), AM. CHEM. SOC.,WASHINGTON,DC,US;, pages 7344 - 7350
C.C. CHANG AND T.Y. CHANG: "Molecular cloning and functional expression of human acyl coenzyme A: cholesterol acyltransferase (ACAT) cDNA", FASEB JOURNAL, vol. 7, no. 7, 10 April 1993 (1993-04-10), ROCKEVILLE PIKE,BETHESDA, MD, US;, pages A1147
C.C.Y. CHANG ET AL.: "Molecular cloning and functional expression of human acyl-coenzyme A: cholesterol acyltransferase cDNA in mutant chinese hamster ovary cells", J. BIOL. CHEM., vol. 268, no. 28, 5 October 1993 (1993-10-05), AM. SOC. MOL. BIOL., INC.,BALTIMORE, US;, pages 20747 - 20755
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